Comparative study on three locally developed live orf virus vaccines for sheep in Saudi Arabia

Housawi, F. M. T.; Abuelzein, Eltayb; Gamee, Ahmed A.; Al-Afaleq, A. I.

doi:10.4102/ojvr.v79i1.397

Cited by 5 publications

(6 citation statements)

References 6 publications

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“…Meanwhile, many researchers have studied the effects of ORFV on various cell cultures. Housawi et al ( 2012 ) compared the immunogenicity and protective efficacy of three locally developed live ORFV vaccines in Vero cell culture. For production of a live attenuated vaccine against ORFV, a wild virus strain has been attenuated through serial passages on the primary CEF tissue cultures (Mercante et al 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and phylogenetic analysis of contagious ecthyma virus from camels (<i>Camelus dromedarius</i>) in Iran

Oryan

Mosadeghhesari

Zibaee

et al. 2017

Onderstepoort j. vet. res.

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Contagious ecthyma is a highly contagious disease affecting domestic and wild ruminants such as sheep, goats and camels. The identification and characterisation of a parapoxvirus (PPV) infecting camels is described here. The virus was detected in dromedary camels (Camelus dromedarius) from Kerman and Shiraz in Iran. PPV-specific amplification by polymerase chain reaction (PCR) further confirmed that the disease was associated with PPV infection. Phylogenetic analysis of ORF011 (B2L) gene sequences showed 99.79% and 82.13% similarity of the PPV identified in this study with the Jodhpur isolate and the bovine papular stomatitis virus (BPSV) isolates (CE41), respectively. Moreover, phylogenetic analysis of the ORF045 gene indicated that the Shiraz sample was in all probability closely related to VR634 and to F00.120R and PCPV776. In conclusion, the results suggest that camel PPV (CPPV) is a likely cause of contagious ecthyma in dromedary camels in Iran.

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Section: Discussionmentioning

confidence: 99%

Identification and phylogenetic analysis of contagious ecthyma virus from camels (<i>Camelus dromedarius</i>) in Iran

Oryan

Mosadeghhesari

Zibaee

et al. 2017

Onderstepoort j. vet. res.

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“…In malignant form of disease, tissue culture derived vaccine with an interferon inducer gave promising results and was superior to traditional vaccine (Valder et al, 1979). Immunogenicity and protective efficacy of three locally developed live orf virus vaccines with field strain of orf virus was assessed in Saudi Arabia over Noemi sheep for direct comparison of vaccine efficacy (Housawi et al, 2012).…”

Section: Prevention and Controlmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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| Contagious pustular dermatitis (CPD), also known as Orf or contagious ecthyma is an important viral disease of sheep and goats. It is mainly seen as a benign disease but malignant form has also been reported from few parts of the world. The rates of morbidity and mortality are higher, particularly in lambs and kids experiencing the disease for the first time. The causative agent of disease is Orf virus, type species of the genus Parapoxvirus belonging to family Poxviridae. The virus produces localized persistent proliferative skin lesions and affected hosts are infected repeatedly owing to its host immune evasive strategy. These viruses have been found uniformly labile to chloroform but resistant to ether and also exhibit antigenic variations among them. Clinically, the disease is enzootic and occurs in three forms viz. labial, genital / mammary and generalized forms. The incubation period in natural cases is 2-3 weeks. The disease outbreaks mostly occur between autumn and spring but its severity is more in autumn and winter than spring. The virus grows well in cell cultures of caprine, ovine and bovine origin. Confounding symptoms impose laboratory affirmation by serological assays or molecular techniques. The disease can be diagnosed by electron microscopy, serological tests and PCR/ quantitative real-time PCR. Virus specific antibody response to structural proteins of capripox and orf viruses in western-blot analysis readily differentiates these two infections. The antibody response to the 32 kDa and 26 kDa proteins of capripox viruses provides a firm basis for differentiation. Although, the role of humoral immunity is well established but probably cell mediated immunity plays a major role in recovery from natural infections. A number of inactivated and live or live modified vaccines have been tried with variable success. The duration of immunity after vaccination is controversial; outbreaks have occurred in vaccinated animals. The disease is also of public health significance as it causes infection in human beings. Keywords

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“…There was multifocal intracellular edema of keratinocytes of the stratum spinosum. The (Ishii, 1953;Merwin and Brundige, 1982;Housawi et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Orf Virus Infection in Wild Goats (Capra Aegagrus Erxleben 1777) of Saricicek Mountain

Gündoğdu¹,

Arpacık²,

Sari³

et al. 2018

Appl. Ecol. Env. Res.

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Orf virus occurs where sheep and goats are raised, and it also infects a broad range of wild artiodactyls. The rates of morbidity and mortality are higher, particularly in lambs and kids experiencing the disease for the first time. Orf virus has been reported in domestic animals and humans in Turkey, so far. In this study, findings on the presence of Orf virus on wild goats were researched for the first time. Field studies were carried out between 2016 and 2017 years. Direct observation methods were used in field studies. In this study, a dead six months of age male wild goat (Capra aegagrus) and 3 diseased individuals in poor condition with bloody lesions covering the muzzle area were examined in Sarıçiçek Mountain in Eastern Black Sea Region, Turkey. The gross and microscopic lesions confirmed the diagnosis of contagious ecthyma (CE). This is the first documented report of CE in wild goat from Turkey.

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Comparative study on three locally developed live orf virus vaccines for sheep in Saudi Arabia

Cited by 5 publications

References 6 publications

Identification and phylogenetic analysis of contagious ecthyma virus from camels (<i>Camelus dromedarius</i>) in Iran

Identification and phylogenetic analysis of contagious ecthyma virus from camels (<i>Camelus dromedarius</i>) in Iran

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Orf Virus Infection in Wild Goats (Capra Aegagrus Erxleben 1777) of Saricicek Mountain

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