Comparative study on the nuclear DNA content of leiomyoma, leiomyoblastoma and leiomyosarcoma of the stomach and small intestine

Kimura, Osamu; Kaibara, Nobuaki; Tamura, Hideaki; Nishidoi, Hideaki; Okamoto, Tsuneyuki; Koga, Shigemasa

doi:10.1007/bf02469026

Cited by 17 publications

(7 citation statements)

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“…The increase was statistically signant tumours (2, 15,18). Flow cytometric studies of bone nificant (p < 0.01).…”

Section: Follow-upmentioning

confidence: 93%

“…All histologically Cell cycle analysis revealed an increased proportion of in the modal DNA values of chondrosarcomas (11,ZO-proliferative cells (i.e., cells in Sand GZM-phases) in the 22,241, osteosarcomas (20-221, as well as other malig-malignant tumours. The increase was statistically signant tumours (2, 15,18). Flow cytometric studies of bone nificant (p < 0.01).…”

Section: Follow-upmentioning

confidence: 93%

“…For some years it has been known that malignant tumours contain cells with an abnormal nuclear DNA content (2, 11,15,18,[20][21][22]24), and this has been confirmed by flow cytometry (FCM) (1,3-6,8-10), 12- Kreicbergs has also studied cells from some other bone tumours (23). In addition the DNA content of myeloma cells has been measured (4, 6,9).…”

mentioning

confidence: 99%

“…For some years it has been known that malignant tumours contain cells with an abnormal nuclear DNA content (2, 11,15,18,[20][21][22]24), and this has been confirmed by flow cytometry (FCM) (1,3-6,8-10), 12- 14,16,17,19-23,25,29,31,34-36). There has been limited investigation of the DNA content of bone tumour cells.…”

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confidence: 99%

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Flow cytometric determination of DNA content in malignant and benign bone tumours

1985

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The nuclear DNA content of 38 malignant and 25 benign bone tumours was measured by flow cytometry. The specimens were taken either from biopsies or from surgical specimens. Seventeen of 26 primary malignant bone tumours were aneuploid, 15 had a single aneuploid DNA content, and 2 had a biclonal abnormality. Thirteen of 15 osteosarcomas were aneuploid, but only 2 of 6 chondrosarcomas showed an aneuploid DNA content. Six of 12 metastatic malignant bone tumours were also aneuploid. All 25 benign tumours had a diploid DNA content. Cell cycle analysis showed that the proportion of cells in S-and G2M-phases was higher in the malignant compared to benign tumours, indicating a higher proliferative activity. The increase was statistically significant (p < 0.05) both in diploid and in aneuploid tumours. Among five tumours studied after chemotherapy, four displayed a marked hyperdiploid abnormality. Aneuploidy and high proliferative activity both were highly associated with malignant bone tumours, suggesting that DNA flow cytometry may be an adjunct in the assessment of malignancy of bone tumours.Key terms: DNA neoplasm, flow cytometry, bone neoplasms, aneuploidyThe benign or malignant nature of bone tumours is usually assessed by the clinical presentation, radiological and laboratory findings, and above all by the histologic examination of biopsies. This provides an estimate of the growth pattern, cellularity, number of mitoses, nuclear pleomorphy, etc. The evaluation requires experience, and because bone tumours are relatively rare, expert consultation may be required.For some years it has been known that malignant tumours contain cells with an abnormal nuclear DNA content (2,11,15,18,(20)(21)(22)24), and this has been confirmed by flow cytometry (FCM) (1,3-6,8-10), 12-14,16,17,19-23,25,29,31,34-36). There has been limited investigation of the DNA content of bone tumour cells. Most studies are on cartilage tumours (1,20-22,241. Kreicbergs has also studied cells from some other bone tumours (23). In addition the DNA content of myeloma cells has been measured (4,6,9). In all these studies aneuploidy has been attributed to tumour malignancy.The aim of the present investigation was to determine whether measurements of nuclear DNA content by FCM could be of value for the assessment of the malignancy of bone tumours.sinki University Central Hospital. All patients were suspected of having bone tumours. More than one specimen on different occasions was obtained from 11 patients. Thus a total of 75 specimens were obtained, either by routine biopsy or at the time of the sur 'cal procedure. The specimens measured about 1 cm? and they were immediately immersed in 0.9% saline.Tissue specimens were minced within 1 h and teased apart in phosphate buffered saline. The cell suspension was filtered through cotton wool and centrifuged for 7 min at lOOg, then resuspended in a buffer solution containing 50 pg/ml ethidium bromide (Sigma Chemical Co, St. Louis, MO) in 0.3% Nonidet P-40 (BDH-chemicals, Poole, England), lOmM Tris, and 2 mM EDTA ...

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“…The increase was statistically signant tumours (2, 15,18). Flow cytometric studies of bone nificant (p < 0.01).…”

Section: Follow-upmentioning

confidence: 93%

Section: Follow-upmentioning

confidence: 93%

mentioning

confidence: 99%

“…For some years it has been known that malignant tumours contain cells with an abnormal nuclear DNA content (2, 11,15,18,[20][21][22]24), and this has been confirmed by flow cytometry (FCM) (1,3-6,8-10), 12- 14,16,17,19-23,25,29,31,34-36). There has been limited investigation of the DNA content of bone tumour cells.…”

mentioning

confidence: 99%

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Flow cytometric determination of DNA content in malignant and benign bone tumours

1985

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“…18 Recent attempts have been made to evaluate the degree of malignancy of this tumor using immunohistologic stains or DNA flow cytometry. [24][25][26] An early establishment of indices for the prediction of prognosis and the degree of malignancy is desirable.…”

Section: Discussionmentioning

confidence: 99%

Successful resection of a liver metastasis from gastric leiomyoblastoma: Report of a case

et al. 2000

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A 20-year-old woman was referred to our hospital for detailed investigation of a gastric submucosal tumor. A leiomyoma was preoperatively diagnosed and laparoscopic-assisted enucleation was performed. The resected tumor was 4 x 3 x 1.5 cm in size and postoperative histological examination identified it as a gastric leiomyoblastoma. Therefore, a secondary resection in the form of a distal gastrectomy was carried out. No tumor cells were found in the gastric specimen or in the lymph nodes; however, 5 months after the operation, an abdominal computed tomography scan revealed a recurrence in the liver, and she was readmitted for further examinations. The lesion was diagnosed as a single liver metastasis from the gastric leiomyoblastoma and successfully resected. The histopathological findings of the liver tumor resembled those of the primary gastric tumor. Her postoperative course was uneventful and she has been well, without any evidence of recurrence, to date. Only 12 other cases of leiomyoblastoma of the stomach with liver metastasis have been reported in Japan, all of which were associated with a very poor prognosis. Therefore, patients with this unusual disease entity should be carefully followed up after resection of the primary tumor.

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Nuclear DNA content in dimethylhydrazine-induced colonic carcinoma and mucosal dysplasia in rats

Kimura¹,

Kaibara²,

Miyano³

et al. 1984

Cancer

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Mucosal dysplasia and carcinoma infiltrating the submucosal layer were induced in rats with dimethylhydrazine. The nuclear DNA content of the cells was measured, and the correlation between the DNA distribution pattern and histopathologic findings was evaluated. In mild and moderate dysplasias, histograms of the nuclear DNA content showed a narrow range of distribution with a sharp peak in the diploid area. In severe dysplasia, however, there was wide distribution and no peak value; this was reminiscent of carcinoma. Based on the findings of this report, the authors suggest that severe dysplasia be regarded as intramucosal carcinoma.

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Comparative study on the nuclear DNA content of leiomyoma, leiomyoblastoma and leiomyosarcoma of the stomach and small intestine

Cited by 17 publications

References 3 publications

Flow cytometric determination of DNA content in malignant and benign bone tumours

Flow cytometric determination of DNA content in malignant and benign bone tumours

Successful resection of a liver metastasis from gastric leiomyoblastoma: Report of a case

Nuclear DNA content in dimethylhydrazine-induced colonic carcinoma and mucosal dysplasia in rats

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