Comparative Study on the Characteristics of Weissella cibaria CMU and Probiotic Strains for Oral Care

Abstract

Abstract:Probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. The present work was conducted to compare the characteristics of oral care probiotics: Weissella cibaria CMU (Chonnam Medical University) and four commercial probiotic strains. Survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and inhibition of volatile sulfur compounds were evaluated. The viability of W. cibaria CMU was not affected by treatment of 100 mg/L lysozyme for 90 min and 1 mM hydrogen peroxide for 6 h. Interestingly, W. cibaria produced less acid and more hydrogen peroxide than the other four probiotics. W. cibaria inhibited biofilm formation by Streptococcus mutans at lower concentrations (S. mutans/CMU = 8) and efficiently coaggregated with Fusobacterium nucleatum. W. cibaria CMU and two commercial probiotics, including Lactobacillus salivarius and Lactobacillus reuteri, showed high antibacterial activities (>97%) against cariogens (S. mutans and Streptococcus sobrinus), and against periodontopathogens (F. nucleatum and Porphyromonas gingivalis). All of the lactic acid bacterial strains in this study significantly reduced levels of hydrogen sulfide and methyl mercaptan produced by F. nucleatum and P. gingivalis (p < 0.05). These results suggest that W. cibaria CMU is applicable as an oral care probiotic.

Ecological Approaches to Dental Caries Prevention: Paradigm Shift or Shibboleth?

“…Thus, while displacing S. mutans from plaque biofilms may in principle be desirable, substituting them for even more cariogenic bacteria will not be useful [Schwendicke et al, 2014a]. Some of the non-acidogenic alternatives to Lactobacillus and Bifidobacterium that have shown promising early results include S. salivarius M18 [Burton et al, 2013], heat-inactivated BB12 [Schwendicke et al, 2014b], and Weissella cibaria CMU [Jang et al, 2016].…”

Potential Use of Biotherapeutic Bacteria to Target Colorectal Cancer-Associated Taxa

“…There is little doubt that targeting this organism in the GI tract and that the potential development of microbiota-targeted therapies against F. nucleatum are promising research avenues. As F. nucleatum was first identified as a pathogen associated with periodontal disease, numerous studies demonstrate the potential use of oral strains to inhibit many dental pathogens in vitro, including strains of F. nucleatum [88][89][90][91]. For example, bio-yogurt was shown to have antimicrobial activity against a range of periodontal pathogens, including the F. nucleatum strains tested.…”

“…Weissella cibaria CMU and two probiotic species isolated from commercial probiotic products, Lactobacillus salivarius and Lactobacillus reuteri, showed antimicrobial activity against F. nucleatum with more than 95% growth inhibition compared with other probiotic strains. Interestingly, W. cibaria CMU demonstrated the highest co-aggregation rate with F. nucleatum with 81.2% as compared with other cariogenic and periodontopathic bacteria [91]. Co-aggregation, a beneficial property of probiotics, is a process of attachment which influences multi-species biofilm formation and is considered a beneficial property of probiotic and other biotherapeutic strains, which may result in barrier formation that prevents colonisation of pathogenic bacteria [92].…”

Enhancement of Viability of Weissella cibaria CMU by Low-Temperature Encapsulation

“…하지만 치아우식증 유발 세균을 억제하기 위해 가장 많이 사용되는 불소화합물은 과도한 섭취 시 복통과 반점치 및 암을 유발할 수 있으며 (Spencer and Do, 2008), 껌이나 캔디 제형으로 제조 가능 한 자일리톨의 경우, 일시적인 구취억제 효과는 충분히 기대할 수 있으나(Aluckan and Ankola, 2018), 제품이 닿지 않는 사각지대까지 제어할 수 없을 뿐더러 자일리톨은 하루에 상당한 양을 섭취 해야 충치유발세균(Streptococcus mutans)의 생육이 억제 가능하다는 단점이 있다 (Li and Tanner, 2015). 본 연구에서는 기존 구강 관리법의 문제점을 보완, 대체하기 위한 방법으로 구강 내에 정착하여 서식 하면서 구취와 충치를 억제하고, 치주질환을 일으키는 다양한 유해세균를 선택적으로 제어할 수 있는 구강 프로바이오틱스(probiotics) 균주인 Weissella cibaria CMU 균주를 사용하였다 (Jang et al, 2016;Kang et al, 2017;Do et al, 2018 (Iravani et al, 2015;Ansari et al, 2017). 현재 분무건조 및 고압균질기 등을 이용한 다양한 캡슐화 방법이 고안되어 왔으나, 일부 고온 처리 방식의 경우 유산균을 사멸시키 는 부작용으로 인해 한계점을 갖게 되었다 (Bae and Kim, 2008;Kim et al, 2013;Kim and You, 2017 (Adhikari et al, 2003 ;Muthukumarasamy et al, 2006 (Fig.…”