comparative Study on Surface Geometric Properties of Decellularized and Glutaraldehyde fixed Bovine Pericardium and Possible Effects on Cell Seeding

Ahmadpour, Shahriar; Golshan, Alireza; Memarian, Toktam

doi:10.21608/jmh.2018.4541.1035

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…A decrease in the Young’s modulus when measured by AFM correlated with the increase in the porosity in the aortic walls revealed in histological study. It is known that an increase in transplant porosity contributes to recellularization [ 48 ] and angiogenesis [ 49 , 50 ]. Yet, according to the scanning electron micrographs, the histoarchitecture of the valve leaflets was preserved.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

et al. 2020

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from a donor tissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assisted processing has been proposed to improve the outcome of decellularization, reduce contamination and time costs. The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, the decellularization of heterogeneous 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the effectiveness of decellularization approaches based on various combinations of these parameters. The protocols were based on the combinations of treatments in alkaline, ethanol or detergent solutions with scCO2-assisted processing at different modes. Histological analysis demonstrated favorable effects of the preconditioning in a detergent solution. Following processing in scCO2 medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimate tensile strength and Young’s modulus of the aortic valve leaflets, while the integrity of the extracellular matrix was preserved.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

et al. 2020

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“…A decrease in the Young's modulus when measured by atomic force microscopy correlated with the increase in the porosity in the aortic wall revealed in histological study. It is known that an increase in transplant porosity contributes to cellularization [39] and angiogenesis [40]. Yet, the histoarchitecture of valve leaflets was preserved.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

Gafarova¹,

Grebenik²,

Lazhko³

et al. 2020

Preprint

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One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from donor tissue reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2) extraction can significantly improve the outcome of decellularization, reduce contamination and time costs.The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, decellularization of complex 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, type of co-solvent, values of pressure and temperature insight the scCO2 reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the efficiency of decellularization using approaches based on various combinations of these parameters. The protocols were based on combinations of treatment in alkaline, ethanol or detergent solutions with scCO2 extraction at different modes. Based on a histological analysis, we have selected an optimal protocol for the decellularization of ovine aortic root employing preconditioning in a detergent solution. The positive effects of scCO2 on the decellularization extent, cytotoxicity and histoarchitecture of the tissue were demonstrated.

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“…In the ABP-treated wounds, the ABP fragment was sliced roughly 0.5 cm larger than the wound and washed for 15 min in a saline-containing penicillin antibiotic solution (Ahmadpour et al 2018 ). The wound was subsequently dried between two sterile gauze wipes before suturing ABP (Fig.…”

Section: Experimental Studymentioning

confidence: 99%

Acellular bovine pericardium as a biological dressing for treatment of cutaneous wounds of the distal limb in donkeys (Equus Asinus)

et al. 2022

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This research was performed to determine the impact of repeated topical dressing with acellular bovine pericardium (ABP) on healing distal limb wounds in donkeys. Twelve male clinically healthy donkeys were subjected to general anesthesia, and full-thickness wounds of six cm2 (2 × 3 cm) were created on the middle dorsolateral surface of the metacarpi. Two defects were made on each donkey’s forelimbs; the right limb was considered a control wound, and the left one was considered a treated wound. Moreover, the control wounds were irrigated with saline every three days postoperatively and bandaged with a standard dressing. The treated wounds were covered with ABP dressings. The ABP dressing was reapplied thrice at 7-, 14- and 21-days post-wound induction. In addition, the wound healing process was monitored clinically, histopathologically, and immunohistochemically of tissue as growth factor-β1, epidermal growth factor receptor, and vascular endothelial growth factor. Besides, the gene expression profile of angiogenic and myofibroblastic genes was applied as vascular endothelial growth factor-A, collagen type 3α1, fibroblast growth factor 7, and the transforming growth factor-β1.The results revealed that the wounds treated with ABP healed more quickly than the control wounds. Additionally, the mean days required for healing were significantly shorter in the ABP-treated wounds (p < 0.05; 69.5 ± 1.6) compared to control wounds (86.3 ± 3). Furthermore, immunohistochemical and gene expression analyses were significantly improved in ABP wounds than in control wounds. In conclusion, ABP is considered a natural biomaterial and promotes the healing of distal limb wounds in donkeys if applied weekly during the first three-week post-wound induction.

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comparative Study on Surface Geometric Properties of Decellularized and Glutaraldehyde fixed Bovine Pericardium and Possible Effects on Cell Seeding

Cited by 5 publications

References 32 publications

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Evaluation of supercritical CO2 extraction protocols in a model of ovine aortic root decellularization

Acellular bovine pericardium as a biological dressing for treatment of cutaneous wounds of the distal limb in donkeys (Equus Asinus)

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