Comparative Study on Reagents Involved in Grape Bud Break and Their Effects on Different Metabolites and Related Gene Expression during Winter

Khalil-Ur-Rehman, Muhammad; Wu, Wei; Xu, Yanshuai; Haider, Muhammad Salman; Li, Chunxia; Tao, Jianmin

doi:10.3389/fpls.2017.01340

Cited by 24 publications

(12 citation statements)

References 52 publications

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“…In many cases, the determination of dormancy or sprouting mainly depends on the balance between ABA and GA levels. HC treatment leads to a decrease in the endogenous ABA level by promoting ABA degradation and inhibiting ABA synthesis, as shown in grape [11] and sweet cherry [6]. In this study, ABA concentration decreased gradually with bud breaking in the HC treatment group (Fig.…”

Section: Discussionsupporting

confidence: 58%

“…Many experiments have shown that H 2 O 2 content increases in buds with HC treatment [4, 6, 11], which is the major factor responsible for bud break. H 2 O 2 treatment inhibits CAT activity, which then generates respiratory stress, leading to cell hypoxia and anaerobic respiration [14].…”

Section: Discussionmentioning

confidence: 99%

“…Exogenous HC application increases the expression level of sucrose synthase, pyruvate decarboxylase, a sucrose non-fermenting (SNF)-like protein kinase, grape dormancy breaking-related protein kinase, and alcohol dehydrogenase [9, 10]. Abscisic acid (ABA) also inhibits dormancy release in grape buds and attenuates the advancing effect of HC [11]. HC can promote dormancy release by increasing the content of ethylene and cytokinin [10, 12].…”

Section: Introductionmentioning

confidence: 99%

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Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

et al. 2019

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BackgroundGrape buds exhibit non-uniform, or delayed, break in early spring in subtropical regions because the accumulation of chilling is insufficient. Hydrogen cyanamide (H2CN2, HC) can partially replace chilling to effectively promote bud sprouting and is used widely in warm winter areas. However, the exact underlying mechanism of grape bud release from endodormancy induced by HC remains elusive.ResultsIn this study, the transcriptome of grape winter buds under in vitro conditions following HC and water treatment (control) was analyzed using RNA-seq technology. A total of 6772 differentially expressed genes (DEGs) were identified. Furthermore, the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that starch and sucrose metabolism and plant hormone signaling transduction were most enriched out of the 50 total pathways. HC treatment induced the upregulated expression of sucrose synthase (SUS), sucrose phosphate synthase (SPS), α-amylase (AM), and β-amylase (BM) and downregulated expression of sucrose invertase (INV), hexokinase (HK), fructokinase (FK), soluble starch synthase (SS), and granule-bound starch synthase (GBSS). Hence, the starch concentration in the HC-treated group was significantly lower than that in control, whereas soluble sugar content in the HC-treated group increased quickly and was higher than that in control between 0 and 8 d. The concentration of indoleacetic acid (IAA) and zeatin (ZT) increased, whereas that of abscisic acid (ABA) and gibberellin (GA) decreased in HC treated group, which coincided with the expression level of genes involved in above hormone signals. The content of hydrogen peroxide (H2O2) and enzyme activity of superoxide dismutase (SOD) and peroxidase (POD) were increased in grape buds with HC treatment, whereas catalase (CAT) activity was decreased. HC treatment increased the expression of POD, SOD, primary amine oxidase (PAO), polyamine oxidase (PAOX), and glutathione peroxidase (GSH-Px).ConclusionBased on these results, it is possible to propose a mechanistic model that underlies the regulation of endodormancy release in grapevine buds by exogenous HC application.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

et al. 2019

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“…Bud dormancy mechanisms in perennial fruits has been previously explored at the molecular level using omics-based approaches [3,5,25,26]. However, the effect of HC in the release of grape dormancy by coupling transcriptomic and proteomic approaches has not been yet investigated.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic and Proteomic Analysis to Deeply Investigate the Role of Hydrogen Cyanamide in Grape Bud Dormancy

Khalil-Ur-Rehman

Dong

et al. 2019

IJMS

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Hydrogen cyanamide (HC) is an agrochemical compound that is frequently used to break bud dormancy in grapevines grown under mild winter conditions globally. The present study was carried out to provide an in-depth understanding of the molecular mechanism associated with HC releasing bud dormancy in grapevines. For this purpose, RNA-seq based transcriptomic and tandem mass tag (TMT)-based proteomic information was acquired and critically analyzed. The combined results of transcriptomic and proteomic analysis were utilized to demonstrate differential expression pattern of genes at the translational and transcriptional levels. The outcome of the proteomic analysis revealed that a total of 7135 proteins (p-value ≤ 0.05; fold change ≥ 1.5) between the treatments (HC treated versus control) were identified, out of which 6224 were quantified. Among these differentially expressed proteins (DEPs), the majority of these proteins were related to heat shock, oxidoreductase activity, and energy metabolism. Metabolic, ribosomal, and hormonal signaling pathways were found to be significantly enriched at both the transcriptional and translational levels. It was illustrated that genes associated with metabolic and oxidoreductase activity were mainly involved in the regulation of bud dormancy at the transcriptomic and proteomic levels. The current work furnishes a new track to decipher the molecular mechanism of bud dormancy after HC treatment in grapes. Functional characterization of key genes and proteins will be informative in exactly pinpointing the crosstalk between transcription and translation in the release of bud dormancy after HC application.

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“…Against a background of a growing number of extensive molecular and biochemical studies of the later stages of bud development in grapevine (Halaly et al, 2008; Ophir et al, 2009; Meitha et al, 2015; Zheng et al, 2015; Sudawan et al, 2016; Khalil-Ur-Rehman et al, 2017; Meitha et al, 2017; Signorelli et al, 2018; Zheng et al, 2018a), few have followed development for an extended period of time, from the onset of dormancy. The most extensive catalogue to date spanned the transcriptional state of the bud from shoot development, prior to the summer solstice and periderm formation, through to bud burst following winter (cv.…”

Section: Introductionmentioning

confidence: 99%

The seasonal dynamics of bud dormancy in grapevine suggest a regulated checkpoint prior to acclimation

Velappan

Considine

et al. 2021

Preprint

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Grapevine (Vitis vinifera L.) displays wide plasticity to climate and seasonality, ranging from strongly deciduous to evergreen. Understanding the physiology of decisions to grow or quiesce is critical for improved crop management, prediction, and the adaptability of production to alternative climate scenarios. The perenniating bud (N+2) is a major economic unit and focus of study. Here we investigated the physiology and transcriptome of cv. Merlot buds grown in a temperate maritime climate from summer to spring in two consecutive years. The changes in bud respiration, hydration and internal tissue oxygen data were consistent with the transcriptome data. ABA-responsive gene processes prevailed upon the transition to a deep metabolic and cellular quiescence in the bud during autumn. Light, together with hypoxia and redox signalling presided over the resumption of nuclear and cellular growth in the transition to spring. Comparisons with transcriptome data from bud burst studies revealed a number of regulatory candidates for the orderly resumption of growth in spring, including components that may integrate light and temperature signalling. Importantly however, the bud burst forcing data, which is widely used as a measure of bud dormancy, were not consistent with the physiological and transcription data. We hypothesise the existence of a physiological checkpoint following bud set in summer, which if not met results in extreme quiescence. Collectively this is the most integrated developmental dataset of the latent bud of cultivated grapevine, and establishes a platform for systems approaches to study seasonal plasticity.

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Comparative Study on Reagents Involved in Grape Bud Break and Their Effects on Different Metabolites and Related Gene Expression during Winter

Cited by 24 publications

References 52 publications

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

Hydrogen cyanamide induces grape bud endodormancy release through carbohydrate metabolism and plant hormone signaling

Comparative Transcriptomic and Proteomic Analysis to Deeply Investigate the Role of Hydrogen Cyanamide in Grape Bud Dormancy

The seasonal dynamics of bud dormancy in grapevine suggest a regulated checkpoint prior to acclimation

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