Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet

Khoa, Trần Nguyễn Duy; Waqalevu, Viliame; Honda, Akinobu; Shiozaki, Kazuhiro; Kotani, Tomonari

doi:10.1016/j.aquaculture.2019.734721

Cited by 13 publications

(8 citation statements)

References 75 publications

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“…This continuous increasing trend was also observed in other herbivorous/omnivorous fish species [7,52]. However, it contrasts to the results obtained in a wide range of marine species with carnivorous habits, where amy2a expression is high during early larval stages and is down-regulated afterward [27,28,42,53,54].…”

Section: Carbohydrate Digestioncontrasting

confidence: 65%

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Gilannejad

Heras

Martos-Sitcha

et al. 2020

Animals

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Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of key digestive enzymes was measured from three until 90 days post hatch (dph). Expression of genes involved in digestion of proteins (try2, ctr, pga2, and atp4a), carbohydrates (amy2a), and lipids (cel and pla2g1b), together with two somatotropic factors (gh and igf1) were also quantified. No chymotrypsin or pepsin activities were detected. While specific activity of trypsin and lipase were high during the first 30 dph and declined afterward, amylase activity was low until 57 dph and increased significantly beyond that point. Expression of try2, ctr, amy2a, and cel increased continuously along development, and showed a peak at the end of metamorphosis. Expression of pla2g1b, pga2 and atp4a increased until the middle of metamorphosis and decreased afterwars. Most of these trends contrast the usual patterns in carnivorous species and highlight the transition from larvae, with high protein requirements, to post-larvae/juvenile stages, with omnivorous/detritivorous feeding preferences. Somatotropic genes, gh and igf1, showed approximately inverse expression patterns, suggesting the establishment of the Gh/Igf1 axis from 50 dph.

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Section: Carbohydrate Digestioncontrasting

confidence: 65%

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Gilannejad

Heras

Martos-Sitcha

et al. 2020

Animals

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“…However, basing on the previous studies, the use of live foods has been proven to be generally expensive and unreliable, such as Tubifex sp. often resulted in pathogen infection [ 12 , 13 ]. Furthermore, previous studies have highlighted that larval growth rates cannot be maintained by using live foods due to their poor nutritive composition [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of weaning American glass eels (Anguilla rostrata) with the formula diet on intestinal microbiota and inflammatory cytokines genes expression

Liang

Liu

Zhai

et al. 2023

Heliyon

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“…To meet the needs of cultured animals, live prey is available in many sizes and nutrient forms [7][8][9]. It was previously reported that when fish larvae ingested compound feed, they died when their intestines were full of food [5,10], which indicated that the larvae could not digest compound feed well and they had insufficient intestinal digestive enzymes [11].…”

mentioning

confidence: 99%

Proteomics-Based Investigation of Different Live Prey Administered to Freshwater Dark Sleeper (Odontobutis potamophila): Examining the Effects on Glycolipids and Energy Metabolism

Zhou,

Jiang,

Zheng

et al. 2024

Metabolites

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Live prey is characterized by balanced rich nutrients and high palatability and is widely used for the seedling cultivation of freshwater dark sleeper (Odontobutis potamophila) larvae. In this study, we evaluated the effects of four groups of paired feeding regimens (group C (Daphnia magna), group L (Limnodrilus hoffmeisteri), group H (Hypophthalmichthys molitrix fry), and group M (mixed groups C, L, and H)) on glycolipid and energy metabolism in O. potamophila larvae. We observed that fatty acid synthase (FAS) and sterol-regulatory-element-binding protein-1 (SREBP-1) mRNA levels were significantly lower in group H when compared to mRNA levels in the other three groups (p < 0.05) and that carnitine palmitoyltransferase 1α (CPT1-α) mRNA levels were significantly lower in group L when compared to group M (p < 0.05). Relative glucokinase (GK) expression levels were significantly lower in group M when compared to the other three groups (p < 0.05). Using proteomics, we analyzed and compared groups H and L and identified 457 differentially expressed proteins (DEPs), of which 151 were significantly up-regulated and 306 were significantly down-regulated. In the comparison of group M with groups C, L, and H, we found significant enrichment in glycolytic processes, the endoplasmic reticulum lumen, NAD binding, intermediate filaments, and nutrient reservoir activity. Our results provide a theoretical guidance for bait selection during larvae cultivation stages in carnivorous fish.

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Comparative study on early digestive enzyme activity and expression in red sea bream (Pagrus major) fed on live feed and micro-diet

Cited by 13 publications

References 75 publications

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Ontogeny of Expression and Activity of Digestive Enzymes and Establishment of gh/igf1 Axis in the Omnivorous Fish Chelon labrosus

Effects of weaning American glass eels (Anguilla rostrata) with the formula diet on intestinal microbiota and inflammatory cytokines genes expression

Proteomics-Based Investigation of Different Live Prey Administered to Freshwater Dark Sleeper (Odontobutis potamophila): Examining the Effects on Glycolipids and Energy Metabolism

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