Comparative study of X-ray treatment and photodynamic therapy by using 5-aminolevulinic acid conjugated gold nanoparticles in a melanoma cell line

Mohammadi, Zahra; Sazgarnia, Ameneh; Rajabi, Omid; Toosi, Mehdi Seilanian

doi:10.3109/21691401.2016.1167697

Cited by 18 publications

(11 citation statements)

References 22 publications

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“…In order to overcome the main limitations of the PDT including low penetration of light into tissues, poor accumulation of the PSs into tumor [33] and improving the efficacy of the photodynamic treatment for cancer management, targeted delivery of PSs to tumor cells is considered as a developing approach in precision medicine [7]. Recent studies have focused on the receptors which are overexpressed on the surface of cancerous cells through which targeted delivery of the drugs to the tumor would be expected [21].…”

Section: Discussionmentioning

confidence: 99%

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Akbarzadeh

Khoshgard

Arkan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Selective accumulation of photosensitizers (PSs) into cancerous cells is one of the most important factors affecting photodynamic therapy (PDT) efficacy. 5-Aminolevulinic acid (5-ALA) is precursor of a strong PS, protoporphyrin-IX (Pp-IX); but it has poor permeability in lipophilic membrane of the cells due to its hydrophilic property. Therefore, establishment of an improved delivery strategy could highly affect on treatment outcome. Moreover, folate receptors (FRs) are overexpressed on the surface of many tumor cells. In the present work, targeting ligand folic acid (FA) and 5-ALA conjugated bismuth oxide nanoparticles (FA-5ALA-Bi 2 O 3 NPs) were synthesized; and used in PDT against human nasopharyngeal carcinoma cells (KB cell line). The KB cells incubated with the synthesized NPs for 2 h; then illuminated using a custom-made red light LED lamp at the light dose of 26 J/cm 2. MTT and caspase-3 activity assays were performed to evaluate the efficacy of treatment. Results showed that FR targeting ligand enables selective endocytosis of FA-5-ALA-conjugated NPs into KB cells. Improved internalization of 5-ALA into cells decreased the cell viability to about 50%, 65%, and 85% in the groups receiving FA-5ALA-Bi 2 O 3 NPs, 5ALA-Bi 2 O 3 NPs, free 5-ALA and subsequent PDT, respectively. Therefore, FA-5ALA-Bi 2 O 3 NPs can significantly increase the cell killing effect of PDT.

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Section: Discussionmentioning

confidence: 99%

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Akbarzadeh

Khoshgard

Arkan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…An advantage of conjugated AuNPs is improved effectiveness of the active ligands bound to the nanoparticle. Several studies have reported a synergistic effect of the active compound when delivered via AuNP compared to the same compound alone [28][29][30].…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

et al. 2020

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Development of nanotechnology has become prominent in many fields, such as medicine, electronics, production of materials, and modern drugs. Nanomaterials and nanoparticles have gained recognition owing to the unique biochemical and physical properties. Considering cellular application, it is speculated that nanoparticles can transfer through cell membranes following different routes exclusively owing to their size (up to 100 nm) and surface functionalities. Nanoparticles have capacity to enter cells by themselves but also to carry other molecules through the lipid bilayer. This quality has been utilized in cellular delivery of substances like small chemical drugs or nucleic acids. Different nanoparticles including lipids, silica, and metal nanoparticles have been exploited in conjugation with nucleic acids. However, the noble metal nanoparticles create an alternative, out of which gold nanoparticles (AuNP) are the most common. The hybrids of DNA or RNA and metal nanoparticles can be employed for functional assemblies for variety of applications in medicine, diagnostics or nano-electronics by means of biomarkers, specific imaging probes, or gene expression regulatory function. In this review, we focus on the conjugates of gold nanoparticles and nucleic acids in the view of their potential application for cellular delivery and biomedicine. This review covers the current advances in the nanotechnology of DNA and RNA-AuNP conjugates and their potential applications. We emphasize the crucial role of metal nanoparticles in the nanotechnology of nucleic acids and explore the role of such conjugates in the biological systems. Finally, mechanisms guiding the process of cellular intake, essential for delivery of modern therapeutics, will be discussed.

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“…In a recent study by Mohammadi Z. et al (2017), 5-aminolevulinic acid (5ALA), a photosensitizer limited by its low uptake by tumor cells, was conjugated with AuNPs and proved to be twice as efficient as 5ALA alone in promoting melanoma cell death [79].…”

Section: Photodynamic Therapymentioning

confidence: 99%

Nanosystems for Improved Targeted Therapies in Melanoma

Beiu

Giurcăneanu

Grumezescu

et al. 2020

JCM

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Melanoma is one of the most aggressive forms of skin cancer, with limited therapeutic options. Since its incidence has been rapidly rising in recent years, the study of new targeted therapeutic strategies has increased. The implication of nanoscience in the development of alternative targeted therapies for melanoma has multiple benefits and could significantly improve the outcome of melanoma patients. In this paper, we review the most recent progress in the field of targeted therapies, emphasizing the impact of nanoscale materials on the targeting and controlled release of anti-tumor drugs. The applications of nanomedicine in the management of melanoma are extensive and refer to sentinel lymph node mapping, chemotherapy, and RNA interference; each of these applications harboring the potential to develop efficient and personalized diagnostic techniques and therapies. Further research, especially in clinical trials, is needed to establish whether fighting melanoma on the nanoscale level represents the key to reaching a critical inflection point in mankind’s battle with metastatic melanoma.

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Comparative study of X-ray treatment and photodynamic therapy by using 5-aminolevulinic acid conjugated gold nanoparticles in a melanoma cell line

Cited by 18 publications

References 22 publications

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Evaluating the photodynamic therapy efficacy using 5-aminolevulinic acid and folic acid-conjugated bismuth oxide nanoparticles on human nasopharyngeal carcinoma cell line

Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery

Nanosystems for Improved Targeted Therapies in Melanoma

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