Comparative study of visible light polymerized gelatin hydrogels for 3D culture of hepatic progenitor cells

Greene, Tanja; Lin, Ting-Yun; Andrisani, Ourania M.; Lin, Chu‐Chieh

doi:10.1002/app.44585

Cited by 49 publications

(79 citation statements)

References 47 publications

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“…We believe that this result might be due to the negative effect of UV irradiation to the cells, which has been shown to cause genomic instability of cells . Previous work from Greene et al describes that hepatocytes photo‐encapsulated in gelatin–norbornene gels using visible light + eosin‐Y had significantly higher metabolic activity compared to their UV counterparts . Caliari et al also showed that UV irradiation significantly reduced the cell viability of hepatic stellate cells when compared to visible light for encapsulation in methacrylated hyaluronic acid hydrogels .…”

Section: Discussionmentioning

confidence: 99%

“…However, one major drawback of using I2959 is that it requires ultraviolet (UV) light for photo‐excitation, which can potentially cause cellular DNA and tissue damage . For example, previous studies conducted by Dahle et al demonstrated that both UVA (320–400 nm) and UVB (290–320 nm) radiation can induce chromosomal, as well as genetic instability in mammalian cells .…”

Section: Introductionmentioning

confidence: 99%

“…In particular, LAP behaves very similarly to I2959, both being type 1 photo‐initiators that undergo unimolecular bond cleavage to generate free radicals to facilitate polymerization (Figure B) . However, LAP has limited molar absorptivity in a narrow visible light range (ε ≈ 30 m −1 cm −1 at 405 nm), resulting in the need of using high concentrations to fabricate hydrogels . On the other hand, although eosin‐Y has a much higher molar absorptivity (ε ≈ 100 000 m −1 cm −1 at 525 nm), it often requires the presence of both a co‐initiator (triethanolamine) and a co‐monomer ( N ‐vinylpyrrolidone or N ‐vinylcaprolactam) to facilitate methacryloyl‐based photo‐polymerization .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Visible Light Cross‐Linking of Gelatin Hydrogels Offers an Enhanced Cell Microenvironment with Improved Light Penetration Depth

Lim

Klotz

Lindberg

et al. 2019

Macromolecular Bioscience

157

172

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In this study, the cyto‐compatibility and cellular functionality of cell‐laden gelatin‐methacryloyl (Gel‐MA) hydrogels fabricated using a set of photo‐initiators which absorb in 400–450 nm of the visible light range are investigated. Gel‐MA hydrogels cross‐linked using ruthenium (Ru) and sodium persulfate (SPS), are characterized to have comparable physico‐mechanical properties as Gel‐MA gels photo‐polymerized using more conventionally adopted photo‐initiators, such as 1‐[4‐(2‐hydroxyethoxy)‐phenyl]‐2‐hydroxy‐2‐methyl‐1‐propan‐1‐one (Irgacure 2959) and lithium phenyl(2,4,6‐trimethylbenzoyl) phosphinate (LAP). It is demonstrated that the Ru/SPS system has a less adverse effect on the viability and metabolic activity of human articular chondrocytes encapsulated in Gel‐MA hydrogels for up to 35 days. Furthermore, cell‐laden constructs cross‐linked using the Ru/SPS system have significantly higher glycosaminoglycan content and re‐differentiation capacity as compared to cells encapsulated using I2959 and LAP. Moreover, the Ru/SPS system offers significantly greater light penetration depth as compared to the I2959 system, allowing thick (10 mm) Gel‐MA hydrogels to be fabricated with homogenous cross‐linking density throughout the construct. These results demonstrate the considerable advantages of the Ru/SPS system over traditional UV polymerizing systems in terms of clinical relevance and practicability for applications such as cell encapsulation, biofabrication, and in situ cross‐linking of injectable hydrogels.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Visible Light Cross‐Linking of Gelatin Hydrogels Offers an Enhanced Cell Microenvironment with Improved Light Penetration Depth

Lim

Klotz

Lindberg

et al. 2019

Macromolecular Bioscience

157

172

View full text Add to dashboard Cite

show abstract

“…While the use of I‐2959 or LAP enables gel crosslinking and stiffening, these cleavage‐type photoinitiators generate highly reactive radicals upon absorbing light at the UV spectrum (e.g., 365 nm) . LAP does absorb light at the visible light wavelength (405 nm); however, high concentration (e.g., ≈6 × 10 −3 m ) is generally required to achieve significant degree of additional crosslinking owing to its low molar absorbability at the visible light wavelengths …”

Section: Introductionmentioning

confidence: 99%

Dynamic PEG–Peptide Hydrogels via Visible Light and FMN‐Induced Tyrosine Dimerization

Liu

Nguyen

Lin

2018

Adv Healthcare Materials

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Photoresponsive hydrogels have become invaluable 3D culture matrices for mimicking aspects of the extracellular matrix. Recent efforts have focused on using ultraviolet (UV) light exposure and multifunctional macromers to induce secondary hydrogel crosslinking and dynamic matrix stiffening in the presence of cells. This contribution reports the design of a novel yet simple dynamic poly(ethylene glycol)–peptide hydrogel system through flavin mononucleotide (FMN) induced di‐tyrosine crosslinking. These di‐tyrosine linkages effectively increase hydrogel crosslinking density and elastic modulus. In addition, the degree of stiffening in hydrogels at a fixed PEG macromer content can be readily tuned by controlling FMN concentration or the number of tyrosine residues built‐in to the peptide linker. Furthermore, tyrosine‐bearing pendant biochemical motifs can be spatial‐temporally patterned in the hydrogel network via controlling light exposure through a photomask. The visible light and FMN‐induced tyrosine dimerization process produces a cytocompatible and physiologically relevant degree of stiffening, as shown by changes of cell morphology and gene expression in pancreatic cancer and stromal cells. This new dynamic hydrogel scheme should be highly desirable for researchers seeking a photoresponsive hydrogel system without complicated chemical synthesis and secondary UV light irradiation.

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“…This mixture can be delivered in a minimally invasive manner and then rapidly crosslinked in situ when exposure to irradiation . Visible light is a more cytocompatible light source than UV light for initiating the crosslinking of cell‐laden hydrogels . So, visible induced free radical photopolymerization is a promising method for preparing this hydrogel in biomaterial applications such as cell therapeutics, wound healing, cartilage/bone regeneration, and drug delivery .…”

Section: Introductionmentioning

confidence: 99%

Co‐initiating function of silk peptide in free radical photopolymerization

Nan

Huang

Shao

et al. 2018

Polymer Engineering & Sci

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Silk peptide used as a co‐initiator in type 2 photo‐initiator has not been known. In this study, the photoinitiation system is composed of the sensitizer, the synergist and silk peptide with low molecular weight (<3,500), which is used to initiate photopolymerization of common monomer. The same photopolymerization was performed to evaluate the co‐initiating ability of four essential amino acids of silk peptide, and of four pentapeptides synthesized using them as substrates, respectively. It was suggested that the glycine residue and alanine residue on silk peptide might be mainly responsible for the co‐initiating role. And the co‐initiating function might be ascribed to the electron/hydrogen donation of silk peptide. Silk peptide combined with the sensitizer and iodonium salt could also efficiently initiate photo‐curing of hydrogel precursors. And due to excellent water solubility of silk peptide, the water absorption of the formed hydrogel could be further improved. These studies will open an opportunity for the application of silk peptide as a biocompatible co‐initiator. POLYM. ENG. SCI., 58:2185–2191, 2018. © 2018 Society of Plastics Engineers

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Comparative study of visible light polymerized gelatin hydrogels for 3D culture of hepatic progenitor cells

Cited by 49 publications

References 47 publications

Visible Light Cross‐Linking of Gelatin Hydrogels Offers an Enhanced Cell Microenvironment with Improved Light Penetration Depth

Visible Light Cross‐Linking of Gelatin Hydrogels Offers an Enhanced Cell Microenvironment with Improved Light Penetration Depth

Dynamic PEG–Peptide Hydrogels via Visible Light and FMN‐Induced Tyrosine Dimerization

Co‐initiating function of silk peptide in free radical photopolymerization

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