Comparative Study of Two Transport Systems for <i>Vibrio Cholerae</i>

Estrada, Santiago; Jaramillo, Edilma; Gaviria, Marcela; Ospina, Sigifredo

doi:10.1177/004947559402400120

Trop Doct

1994

DOI: 10.1177/004947559402400120

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Comparative Study of Two Transport Systems for Vibrio Cholerae

Santiago Estrada¹,

Edilma Jaramillo²,

Marcela Gaviria³

et al.

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Cited by 3 publications

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“…The proportion of positive samples was still high even when culture was performed more than 2 weeks after collection, with no statistically significant differences in proportions of positive samples between transport media (Table 2). These results show that the filter paper method is as effective as use of the recommended Cary-Blair medium for specimen transport for culture in field conditions, confirming the results obtained during earlier studies (6,8).…”

supporting

confidence: 89%

Use of Filter Paper as a Transport Medium for Laboratory Diagnosis of Cholera under Field Conditions

Page¹,

Alberti²,

Guénolé³

et al. 2011

J Clin Microbiol

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confidence: 89%

Use of Filter Paper as a Transport Medium for Laboratory Diagnosis of Cholera under Field Conditions

Page¹,

Alberti²,

Guénolé³

et al. 2011

J Clin Microbiol

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Vibrio cholerae detection: Traditional assays, novel diagnostic techniques and biosensors

Cecchini

Fajs

Cosnier

et al. 2016

TrAC Trends in Analytical Chemistry

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Diagnosis of Vibrio cholerae O1 Infection in Africa

Keddy

Sooka

Parsons

et al. 2013

Journal of Infectious Diseases

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Isolation of Vibrio cholerae O1 is necessary for cholera outbreak confirmation. Rapid diagnostic testing of fecal specimens, based on lipopolysaccharide detection of V. cholerae O1 or O139, may assist in early outbreak detection and surveillance. Cary-Blair transport medium is recommended for specimen transport. Filter paper, although used in epidemics, needs evaluation against rectal swab specimens. Fecal specimens are subcultured onto selective and nonselective media, including 5% blood agar and TCBS agar, for detection of V. cholerae O1 or O139. Suspicious, oxidase-positive isolates are serotyped in monovalent antisera. Antimicrobial-susceptibility testing is performed to detect resistance. Molecular characterization supports phenotypic identification and outbreak investigations. The presence of genes encoding cholera toxin, lipopolysaccharide, and El Tor biotype traits can be confirmed. Standardized pulsed-field gel electrophoresis analysis facilitates strain comparison. Quality management ensures reliability of results through validation and verification of functional laboratory equipment; quality control of testing procedures, laboratory reagents, and consumables; and participation in proficiency-testing schemes.

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Comparative Study of Two Transport Systems for Vibrio Cholerae

Cited by 3 publications

References 0 publications

Use of Filter Paper as a Transport Medium for Laboratory Diagnosis of Cholera under Field Conditions

Use of Filter Paper as a Transport Medium for Laboratory Diagnosis of Cholera under Field Conditions

Vibrio cholerae detection: Traditional assays, novel diagnostic techniques and biosensors

Diagnosis of Vibrio cholerae O1 Infection in Africa

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