Comparative study of two reprocessing methods for formalin fixed paraffin embedded tissue

Lunetta, Matthew; Grivois, Megan; Hansen, Christopher J.; Seery, Peter; Felty, Cameron C; Rizzo, Elizabeth J.; Jackson, Christopher R.

doi:10.1080/01478885.2022.2062532

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…6,8 Fixation is carried out immediately tissues are removed to preserve the shape and structural integrity of the tissue in a chemical 9 and present the tissues in a physical state to withstand subsequent treatments with various reagents. 10,11 The most commonly used fixative is 10% neutral-buffered formalin 12 with the aim of preserving tissues in a life-like condition to prevent autolysis and putrefaction. 7,13 Dehydration removes water from the tissues 14 using increasing grades of alcohol such as 70%, 80%, 90%, and absolute alcohol.…”

Section: Introductionmentioning

confidence: 99%

Clearing Properties Between Coconut Oil and Xylene in Histological Tissue Processing

Bright,

Samuel,

Adisa

et al. 2024

J Histochem Cytochem.

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Xylene is the commonest clearing agent even though it is hazardous and costly. This study evaluated the clearing properties of coconut oil as an alternative cost-effective clearing agent for histological processes. Ten (10) prostate samples fixed in formalin were taken and each one was cut into 4 before randomly separating them into four groups (A, B, C and D). Tissues were subjected to ascending grades of alcohol for dehydration. Group A was cleared in xylene and Groups B, C, and D were cleared at varying times of 1hr 30mins, 3hrs, and 4hrs in coconut oil respectively before embedding, sectioning, and staining were carried out. Gross and histological features were compared. Results indicated a significant shrinkage in coconut oil-treated specimen compared with the xylene-treated specimen and only the tissues cleared in coconut oil for 4hrs were as rigid as the tissues cleared in xylene ( p > 0.05). No significant difference was found in either of the sections when checked for cellular details and staining quality ( p > 0.999). Coconut oil is an efficient substitute for xylene in prostate tissues with a minimum clearing time of 4hrs, as it is environmentally friendly and less expensive, but causes significant shrinkage to prostate tissue:

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