Comparative study of transgenic and non-transgenic maize ( Zea mays ) flours commercialized in Brazil, focussing on proteomic analyses

Vidal, Nádia de Carvalho; Barbosa, Herbert S.; Jacob, Silvana do Couto; Arruda, Marco Aurélio Zezzi

doi:10.1016/j.foodchem.2015.02.051

Cited by 32 publications

(25 citation statements)

References 33 publications

(32 reference statements)

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“…Moreover, the DEPs identified in the transgenic plants were not identified as new proteins but as proteins showing changes in abundance. These results agreed with our findings in maize leaves 22 and the findings of many other studies 39 , 40 , indicating that DEPs were detectable but showed no substantial differences in the seeds of PT versus NT maize after overexpression of target genes 8 , 22 , 41 .…”

Section: Discussionsupporting

confidence: 93%

Proteomic analysis of phytase transgenic and non-transgenic maize seeds

Tan¹,

Zheng²,

Yang³

et al. 2017

Sci Rep

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Proteomics has become a powerful technique for investigating unintended effects in genetically modified crops. In this study, we performed a comparative proteomics of the seeds of phytase-transgenic (PT) and non-transgenic (NT) maize using 2-DE and iTRAQ techniques. A total of 148 differentially expressed proteins (DEPs), including 106 down-regulated and 42 up-regulated proteins in PT, were identified. Of these proteins, 32 were identified through 2-DE and 116 were generated by iTRAQ. It is noteworthy that only three proteins could be detected via both iTRAQ and 2-DE, and most of the identified DEPs were not newly produced proteins but proteins with altered abundance. These results indicated that many DEPs could be detected in the proteome of PT maize seeds and the corresponding wild type after overexpression of the target gene, but the changes in these proteins were not substantial. Functional classification revealed many DEPs involved in posttranscriptional modifications and some ribosomal proteins and heat-shock proteins that may generate adaptive effects in response to the insertion of exogenous genes. Protein-protein interaction analysis demonstrated that the detected interacting proteins were mainly ribosomal proteins and heat-shock proteins. Our data provided new information on such unintended effects through a proteomic analysis of maize seeds.

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Section: Discussionsupporting

confidence: 93%

Proteomic analysis of phytase transgenic and non-transgenic maize seeds

Tan¹,

Zheng²,

Yang³

et al. 2017

Sci Rep

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“…The increasing literature reporting application of omics methods to assess proteome, metabolome and transcriptome profiles in GMO crops shows strong evidence of distinct grain proteomes in other GM maize events, such as MON810 Bt insecticide producing maize111246. Although the majority of studies have focused on insect-resistant maize (e.g., MON810 event) and most likely because this was the first GM maize to enter the food and feed market, there has also been one previous metabolomics study investigating NK603.…”

Section: Discussionmentioning

confidence: 99%

An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process

Mesnage

Agapito-Tenfen

Vilperte

et al. 2016

Sci Rep

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Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as ‘substantially equivalent’ to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.

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“…9,10 In this context, the proteins stand out for its participation in several events in the biological system, where the analysis of possible changes in their expressions has been the main focus of the studies involving proteomics of plants, since they provide valuable information about the status of the biological system as can be observed in the literature. [11][12][13] Therefore, the choice of the sample preparation procedure becomes a fundamental stage of the analytical process and the proper optimization of this step is crucial to the results obtained, since they demonstrate the biological variation and not the presence of systematic errors in the analysis.…”

Section: Introductionmentioning

confidence: 99%

Sample Preparation Applied to Analysis of Proteins in Cowpea Seeds (Vigna unguiculata (L.) Walp.)

Nascimento

Coelho

Júnior

et al. 2018

J. Braz. Chem. Soc.

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In this study, 20 different protocols for protein extraction of cowpea seeds in natura were evaluated taking into account four parameters: cell disruption method, time, temperature and solvent. Factorial design experiments were executed to determine the effects on the total protein content of extracts. The total protein content was determined and the results were in the range of 8.75 ± 1.3 and 86.6 ± 6.9 mg g-1. The richest protein extracts were obtained using Tris-HCl buffer at 50 °C for 20 min for each assessed cell disruption method. However, the results using water were similar to buffer according to t-test at the 95% confidence level. Six protocols demonstrated higher extraction efficiency. Comparative proteomic study using size exclusion chromatography coupled to diode array detector (SEC-DAD) revealed that the one protocol extracted simultaneously high and low molecular mass biomolecules. In fact, the effect of microwave radiation promoted an increased solubility of biomolecules in water. The analysis of chromatographic profiles for principal component analysis (PCA) highlights the greater efficiency of extraction with the application of this protocol. Therefore, our study showed that the optimum condition for extraction of cowpea seed protein consists of water assisted by microwave radiation at 50 °C for 20 min.

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Comparative study of transgenic and non-transgenic maize ( Zea mays ) flours commercialized in Brazil, focussing on proteomic analyses

Cited by 32 publications

References 33 publications

Proteomic analysis of phytase transgenic and non-transgenic maize seeds

Proteomic analysis of phytase transgenic and non-transgenic maize seeds

An integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize reveals metabolism disturbances caused by the transformation process

Sample Preparation Applied to Analysis of Proteins in Cowpea Seeds (Vigna unguiculata (L.) Walp.)

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