Comparative Study of The Yield and Physicochemical Properties of Collagen from Sea Cucumber (Holothuria scabra), Obtained through Dialysis and the Ultrafiltration Membrane

Abstract

Collagen was extracted from the body wall of sea cucumber (Holothuria scabra) using the pepsin-solubilized collagen method followed by isolation using dialysis and the ultrafiltration membrane. The yield and physicochemical properties of the collagen obtained from both isolation methods, denoted as D-PSC and UF-PSC, were compared. The ultrafiltration method affords a higher yield of collagen (11.39%) than that of the dialysis (5.15%). The isolated collagens have almost the same amino acid composition, while their functional groups, referred to as amide A, B, I, II, and III bands, were in accordance with commercial collagen, as verified by Fourier Transform Infrared (FT-IR) spectroscopy. The UV-Vis absorption peaks at 240 nm and 220 nm, respectively, indicated that the collagens produced are type-I collagen. The D-PSC showed interconnecting sheet-like fibrils, while the UF-PSC exhibited a flaky structure with flat-sheets arranged very close to each other. The higher yield and comparable physicochemical properties of the collagen obtained by ultrafiltration as compared with dialysis indicate that the membrane process has high potential to be used in large-scale collagen production for food and pharmaceutical applications.

Improving collagen processing towards a greener approach: current progress

“…The use of UF with regenerated cellulose (RC) membrane (MWCO of 10 kDa) doubled the yield of PSC extraction more than that of salt precipitation. Moreover, when UF was used, the recovery time was shortened by sixfold, and reasonably high purity collagen was attained 32 . Similarly, another study conducted the rapid isolation of large‐scale collagen production from the scale of red drum fish ( Sciaenops ocellatus ) using a hydrophilic UF membrane and extracted collagen with high purity 33 .…”

“…73 Additionally, the study claimed that collagen purification was a required step, resulting in a clear, white agglomerated powder. A longer (120 h) salting-out procedure with 2.5 mol L −1 NaCl in the presence of 0.05 mol L −1 tris(hydroxymethyl) aminomethane (pH 7.0) increased the yield of PSC extraction for collagen from 32 sea bass (Lates calcarifer) scale by 179% compared to ASC extraction. 18 Collagen from different tissue types was precipitated by adding different amounts of salt to the supernatant.…”

“…97 The membrane size for collagen purification ranges from 10 to 30 kDa. 32 Large quantities of solutions or suspensions can be concentrated and filtered with minimum product degradation using UF within a few hours. 98 Figure 3(b) shows a schematic diagram of an ultrafiltration set-up and the separation mechanism of ultrafiltration for collagen recovery.…”

“…Moreover, when UF was used, the recovery time was shortened by sixfold, and reasonably high purity collagen was attained. 32 Similarly, another study conducted the rapid isolation of large-scale collagen production from the scale of red drum fish (Sciaenops ocellatus) using a hydrophilic UF membrane and extracted collagen with high purity. 33 PSC collected and concentrated by hydrophilic UF displayed high electrophoretic purity, comparable to a type I rat-tail collagen standard, and was significantly purer than collagen before the use of hydrophilic UF.…”

Succinylation of Polyallylamine: Influence on Biological Efficacy and the Formation of Electrospun Fibers

“…The amount of SA allowed a control over the charge ratios in ampholytic polyelectrolytes. PAA recovery rates were from 56 wt.% for PAA-0.5SA, 45 wt.% for PAA-1SA, 46 wt.% for PAA-2.5SA, 37 wt.% for PAA-5SA, and 53 wt.% for PAA-10SA and could potentially be improved by alternative purification procedures such as ultrafiltration [27]. The amount of primary amino groups in PAA and all PAA-xSA samples was also determined by polyelectrolyte titration.…”