Comparative Study of the Phosphatase Activity in the Semen of Bovines (Bos bubalis and Bos taurus) in Egypt

Abstract

Summary The paper records the average values of acid and alkaline phosphatases in 102 (first, second and third) ejaculates from buffalo, Friesian, and native bulls maintained at 3 different localities. The levels of these enzymes were studied in both whole semen and seminal plasma. The results were examined to reveal the difference between buffalo and cattle, and between consecutive ejaculates, and also the variation in phosphomonoesterase activity of semen in relation to the locality. Zusammenfassung Vergleichende Untersuchungen über die Phosphatase‐Aktivität im Sperma von Boviden (Bos bubalis und Bos taurus) in Ägypten Die Aktivität der sauren und alkalischen Phosphatase in drei aufeinanderfolgenden Ejakulaten von 15 Büffeln, 6 Friesen‐Bullen und 2 im Lande gezüchteten Baladi‐Bullen wurde untersucht. Die Enzymwerte wurden sowohl im Vollsperma als auch im Spermaplasma bestimmt. Die Ergebnisse wurden einerseits hinsichtlich der Differenzen zwischen den Büffeln und Rindern und anderseits zwischen den aufeinanderfolgenden Ejakulaten ausgewertet. Im weiteren wurden die Unterschiede der Phosphomonoesterase‐Aktivität des Samens hinsichtlich des verschiedenen Standortes der Probanden geprüft. Résumé Etude comparative de l'activité des phosphatases dans le sperme de bovins (Bos bubalis et Bos taurus) en Egypte On a examiné l'activité des phosphatases acides et alcalines dans trois éjaculats successifs de 15 buffles, 6 taureaux frisons et 2 taureaux Baladi élevés dans le pays. On a déterminé les valeurs enzymatiques aussi bien dans le sperme complet que dans le plasma du sperme. On a donné les résultats d'une part en fonction des différences entre les éjaculats successifs. On a testé de plus les différences de l'activité de la phosphomonoesterase du sperme en rapport avec les différents lieux de prélèvement. Resumen Estudio comparativo de la actividad fosfatásica en el semen de bovinos (Bos bubalis y Bos taurus) en Egipto Se analizó la actividad de las fosfatasas ácida y alcalina en tres eyaculados consecutivos de 15 búfalos, 6 toros de la raza frisia y 2 toros baladi autóctonos en 3 localidades. Los valores enzimáticos se valoraron en el esperma íntegro y en el plasma seminal. Los resultados se examinaron para revelar por un lado las diferencias entre los búfalos y los toros, y por otro entre los eyaculados consecutivos. Además se examinaron las variaciones en la actividad de la fosfomonoestearasa del semen en relación con los lugares diferentes en que se hallaban los animales en cuestión.

Enzymic Profile of the Semen of Bovines (Bubalus bubalis and Bos taurus)

“…In agreement with ABDOU et al (1974), buffalo semen was found to display almost 250 O / o and 200 O / o higher acid and alkaline phosphatases, respectively, than Friesian bulls. Although the phosphatases activities, like many other biochemical attributes of semen, may be governed by hereditary factors controlling the functional status of the reproductive organs in which they are synthesized, it seems likely that the semen content of these enzymes may vary greatly depending on the environmetnal conditions available at the time of ejaculation.…”

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Detailed reviews (MANN, 1949;1964) on semen metabolism have emphasized the important role of dephosphorylating enzymes, particularly that of the phosphomonoesterases-acid and alkaline phosphatases, in sperm glycolytic reactions. The levels of seminal phosphatases as well as the relative proportions of the acid and alkaline enzymes are known to vary greatly from one species to another (BELL andLAKE, 1962, andMURDOCH andWHITE, 1968).Whenever comparisons between buffaloes and bulls were set up, it was obvious that the semen of buffaloes displayed significantly much higher activity of both enzymes (ROY et al, 1960;ABDULLA et al, 1973, andABDOU et al, 1974). The high content of phosphatases in buffalo semen together with the high concentration of phosphate esters was thought to favour the accumulation of inorganic phosphorus ions at a level which is likely to inhibit the respiration and viability of spermatozoa (ROY et al, 1960).

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“…Whenever comparisons between buffaloes and bulls were set up, it was obvious that the semen of buffaloes displayed significantly much higher activity of both enzymes (ROY et al, 1960;ABDULLA et al, 1973, andABDOU et al, 1974). The high content of phosphatases in buffalo semen together with the high concentration of phosphate esters was thought to favour the accumulation of inorganic phosphorus ions at a level which is likely to inhibit the respiration and viability of spermatozoa (ROY et al, 1960).…”

Leakage of Phosphatases and Fertility of Buck Semen Cryopreserved under Different Freezing Modes

“…Phosphatases are found in seminal plasma, acrosomes, sub-acrosomal space, post nuclear cap, cytoplasmic droplets and tail specially the mid piece of spermatozoa (Guraya, 1987). Seminal plasma phosphatases are derived from the secretions of accessory sex glands (Abdou et al, 1974) and are involved in transport of calcium ions from external fluids of female genital tract (Restall and Wales, 1968).…”

Enzymic Profile of the Semen of Bovines (Bubalus bubalis and Bos taurus) I. Acetylcholinesterase Activity

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Reports on the enzymic composition of buffalo semen are few. Studies on the levels of some enzymes, particularly acid and alkaline phosphatases (ROY et al, 1960;ABDULLA et al, 1973 andABDOU et al, 1974), transaminases, lactic dehydrogenase and aldolase (CHAUHAN and SRIVASTAVA, 1973) revealed a picture quite different from that for bull semen. The difference in the activities of these and probably other enzymes may be responsible for the different biological behaviour and preservability of the semen of the two species.

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