Comparative Study of the Morphological Changes in Lymphocytes of Elderly Individuals and Centenarians

Beregi, E; Regius, O; Rajczy, Katalin

doi:10.1093/ageing/20.1.55

Cited by 22 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results are in agreement with others previously reported in the literature. For instance, Beregi et al (1991) [52] examined “in vivo” some immunological variables from peripheral blood lymphocytes in centenarians and healthy subjects (60–89 years-old). The results showed the presence of lipofuscin in the cytoplasm of lymphocytes that significantly increased with age.…”

Section: Discussionmentioning

confidence: 99%

Role of macrophages in age-related oxidative stress and lipofuscin accumulation in mice

et al. 2017

View full text Add to dashboard Cite

The age-related changes in the immune functions (immunosenescence) may be mediated by an increase of oxidative stress and damage affecting leukocytes. Although the “oxidation-inflammation” theory of aging proposes that phagocytes are the main immune cells contributing to “oxi-inflamm-aging”, this idea has not been corroborated. The aim of this work was to characterize the age-related changes in several parameters of oxidative stress and immune function, as well as in lipofuscin accumulation (“a hallmark of aging”), in both total peritoneal leukocyte population and isolated peritoneal macrophages. Adult, mature, old and long-lived mice (7, 13, 18 and 30 months of age, respectively) were used. The xanthine oxidase (XO) activity-expression, basal levels of superoxide anion and ROS, catalase activity, oxidized (GSSG) and reduced (GSH) glutathione content and lipofuscin levels, as well as both phagocytosis and digestion capacity were evaluated. The results showed an age-related increase of oxidative stress and lipofuscin accumulation in murine peritoneal leukocytes, but especially in macrophages. Macrophages from old mice showed lower antioxidant defenses (catalase activity and GSH levels), higher oxidizing compounds (XO activity/expression and superoxide, ROS and GSSG levels) and lipofuscin levels, together with an impaired macrophage functions, in comparison to adults. In contrast, long-lived mice showed in their peritoneal leukocytes, and especially in macrophages, a well-preserved redox state and maintenance of their immune functions, all which could account for their high longevity. Interestingly, macrophages showed higher XO activity and lipofuscin accumulation than lymphocytes in all the ages analyzed. Our results support that macrophages play a central role in the chronic oxidative stress associated with aging, and the fact that phagocytes are key cells contributing to immunosenescence and “oxi-inflamm-aging”. Moreover, the determination of oxidative stress and immune function parameters, together with the lipofuscin quantification, in macrophages, can be used as useful markers of the rate of aging and longevity.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of macrophages in age-related oxidative stress and lipofuscin accumulation in mice

et al. 2017

View full text Add to dashboard Cite

show abstract

“…120 In long-term culture of lymphocytes, a significant increase with aging in the percentage of cells presenting at least one autophagic inclusion was observed. Furthermore, a progressive increase of lipofuscin specific autofluorescence and more autophagic vacuoles were found as cells acquired replicative senescence characteristics.…”

Section: Immunosenescence: a Particular Example Of Consequences Of Aumentioning

confidence: 97%

Autophagy and Aging: The Importance of Maintaining "Clean" Cells

et al. 2005

View full text Add to dashboard Cite

Previously published online as an Autophagy E-publication: http://www.landesbioscience.com/journals/autophagy/abstract.php?id=2017 KEY WORDS Review Autophagy and AgingThe Importance of Maintaining "Clean" Cells ABSTRACTA decrease in the turnover of cellular components and the intracellular accumulation of altered macromolecules and organelles are features common to all aged cells. Diminished autophagic activity plays a major role in these age-related manifestations. In this work we review the molecular defects responsible for the malfunctioning of two forms of autophagy, macroautophagy and chaperone-mediated autophagy, in old mammals, and highlight general and cell-type specific consequences of dysfunction of the autophagic system with age. Dietary caloric restriction and antilipolytic agents have been proven to efficiently stimulate autophagy in old rodents. These and other possible experimental restorative efforts are discussed.

show abstract

“…The typical fluorescence emission spectrum of lipofuscin extends from 570–605 nm when excited at 366 nm 29–32 . Such a spectrum is probably caused by reactions between carbonyls and amino groups that result in Schiff bases, such as 1,4‐dihydropyridines or 2‐hydroxy‐1,2‐dihydropyrrol‐3‐ones, showing an autofluorescence that is similar to that of lipofuscin 21 . In retinal pigment epithelial cells, a lipofuscin fluorophore (A2‐E) was found showing a Schiff base structure (N‐retinyl‐N‐retinylidene ethanolamine).…”

Section: Lipofuscin—an Introductionmentioning

confidence: 99%

Lipofuscin

Jung

Bader²,

Grune³

2007

Annals of the New York Academy of Sciences

372

245

View full text Add to dashboard Cite

One of the highlights of postmitotic aging is the intracellular accumulation of highly oxidized and cross-linked proteins, known as lipofuscin. Lipofuscin is insoluble and not degradable by lysosomal enzymes or the proteasomal system, which is responsible for the recognition and degradation of misfolded and oxidatively damaged proteins. These aggregates have been found in various cell types, including heart, liver, kidney, neuronal tissue, and dermal tissue, and are associated with the life span of a single postmitotic cell and, consequently, of the whole organism. Lipofuscin formation appears to depend on the rate of oxidative damage to proteins, the functionality of mitochondrial repair systems, the proteasomal system, and the functionality and effectiveness of the lysosomes. This review highlights the current knowledge of the formation, distribution, and effects of lipofuscin in mammalian cells.

show abstract

Comparative Study of the Morphological Changes in Lymphocytes of Elderly Individuals and Centenarians

Cited by 22 publications

References 0 publications

Role of macrophages in age-related oxidative stress and lipofuscin accumulation in mice

Role of macrophages in age-related oxidative stress and lipofuscin accumulation in mice

Autophagy and Aging: The Importance of Maintaining "Clean" Cells

Lipofuscin

Contact Info

Product

Resources

About