Comparative study of the morphological, physiological and molecular characteristics between diploid and tetraploid radish (Raphunas sativus L.)

Pei, Yun; Yao, Nan; He, Lei; Deng, Daixin; Li, Wei; Zhang, Wanping

doi:10.1016/j.scienta.2019.108739

Cited by 33 publications

(24 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our evaluations revealed that tetraploids displayed a higher proportion of dry mass per leaf area, which is consistent with their larger cells of the spongy mesophyll. Previous reports correlated dry mass and ploidy in colchicine-induced herbaceous polyploids (Decanter et al, 2020 and references therein), and in some woody polyploids, such as Populus (Zhang et al, 2019) or Lonicera (Li et al, 2009). We further noticed a higher fraction of water per area unit in tetraploids, but leaf bench dehydration rates were slower than diploids, pointing to lower epidermal conductance of tetraploids.…”

Section: Enhanced Leaf Hydration and Water Retention In Tetraploidsmentioning

confidence: 93%

“…Traditionally, agriculture and horticulture have benefited from the use of natural or artificial polyploid genotypes with increased tolerance to stresses such as salinity or drought. Herbaceous polyploid genotypes with these characteristics are present in genera of agronomic interest, such as Lycopersicum (Tal & Gardi, 1976), Raphanus (Pei et al, 2019), or Fragaria (Wei et al, 2018(Wei et al, , 2019, but also in ornamental species such as Phlox (Vyas et al, 2007), Chamaenerion (Maherali et al, 2009), or Saxifraga (Decanter et al, 2020). Comparatively, polyploidy is less frequent in woody species (Ancel Meyers & Levin, 2006), but some results in woody plants point to the capacity of tetraploids to retain more water, such as in seedlings of Betula (Li et al, 1996), and plants of Lonicera (Li et al, 2009), or Populus (Xu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Changes In Ploidy Affect Vascular Allometry And Hydraulic Function In Trees

Barceló-Anguiano

Holbrook

et al. 2021

Preprint

View full text Add to dashboard Cite

The enucleated vascular elements of the xylem and the phloem offer an excellent system to test the effect of ploidy on plant function because variation in vascular geometry has a direct influence on transport efficiency. However, evaluations of conduit sizes in polyploid plants have remained elusive, most remarkably in woody species. We used a combination of molecular, physiological, and microscopy techniques to model the hydraulic resistance between source and sinks in tetraploid and diploid mango trees. Tetraploids exhibited larger chloroplasts, mesophyll cells, and stomatal guard cells, resulting in higher leaf elastic modulus and lower dehydration rates despite the high water potentials of both ploidies in the field. Both the xylem and the phloem displayed a scaling of conduits with ploidy, revealing attenuated hydraulic resistance in tetraploids. Conspicuous wall hygroscopic moieties in the cells involved in processes of transpiration and transport advocates a role in volumetric adjustments due to turgor change in polyploids, which, together with the enlargement of organelles, cells, and tissues that are critical for water and photoassimilate transport at long distances, imply major physiological novelties of polyploidy.

show abstract

Section: Enhanced Leaf Hydration and Water Retention In Tetraploidsmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Changes In Ploidy Affect Vascular Allometry And Hydraulic Function In Trees

Barceló-Anguiano

Holbrook

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Raphunus sativus L. was confirmed after comparing to the diploids, and also differences Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 6 January 2021 doi:10.20944/preprints202101.0110.v1in the endogenous phytohormone levels and flowering genes expression carried out to delay in the flowering and bolting in the polysomic tetraploids[53]. Rao et al[4] reported that the induced polysomic tetraploidy in Lycium ruthenicum resulted plants that exhibited increase in abscisic acid content in relation to the diploid counterparts due to doubled gene copy and upregulated of the 9-cis-epoxycarotenoid dioxygenase 1 and 9-cis-epoxycarotenoid dioxygenase 2 genes involved with this hormone biosynthesis.…”

mentioning

confidence: 86%

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

View full text Add to dashboard Cite

Polyploidy is a condition of having more than two sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological methods (2n gametes). It is considered an interesting approach due to increased genescope and expression, thus resulting in phenotypic changes, such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages from optimized application methods, concentration and time mainly using colchicine. These experimental polyploids proved to be a valuable tool for understanding gene expression that is driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers aided on the identification of rare alleles for particular loci when compared with diploids, which may explain their heterozygosity, phenotypic plasticity and adaptability to diverse environments. It has also been observed that experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g. leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids underwent selection for several generations to improve their performance and stability.

show abstract

“…The "gigas effect" on morphological and physiological traits of polysomic tetraploids in root vegetable radish (Raphunus sativus L.) was confirmed after comparing it to its diploid forms. There were also differences in endogenous phytohormone levels and flowering genes expression carried out to delay flowering and bolting in polysomic tetraploids [67]. Rao et al [4] reported that induced polysomic tetraploid plants in Russian box thorn (Lycium ruthenicum Murray)-used for curing blindness in camels, exhibited more abscisic acid content that its diploid counterparts due to doubled gene copy and upregulated of the 9-cis-epoxycarotenoid dioxygenase 1 and 9-cis-epoxycarotenoid dioxygenase 2 genes involved with this hormone biosynthesis.…”

Section: "Omics" Analysis Of Polyploidymentioning

confidence: 99%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

View full text Add to dashboard Cite

Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

show abstract

Comparative study of the morphological, physiological and molecular characteristics between diploid and tetraploid radish (Raphunas sativus L.)

Cited by 33 publications

References 53 publications

Changes In Ploidy Affect Vascular Allometry And Hydraulic Function In Trees

Changes In Ploidy Affect Vascular Allometry And Hydraulic Function In Trees

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Induced Polyploidy: A Tool for Forage Species Improvement

Contact Info

Product

Resources

About