Comparative study of the microbial activity in different soils by the microcalorimetric method

Barros, Nieves; Feijóo, Sergio; Balsa, R.

doi:10.1016/s0040-6031(97)00071-3

Cited by 38 publications

(16 citation statements)

References 15 publications

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“…On the other hand, our result that samples with the greatest value of Q T showed the smallest value of k (Table 2), was consistent with previous research (Barros et al 1997), which suggested that samples with the greatest percentages of organic matter showed the greatest values of Q T and the smallest values of k. This may be due to a bigger availability of substrate sensible to microbial attack and organic matter suffers a slower decomposition, or may be due to the different vegetation distribution of sampling sites (Zheng et al 2007). Therefore, there was a possible correlation between Q T and organic matter or vegetation quantity.…”

Section: Soil Microbial Activitysupporting

confidence: 93%

“…5b). These results were consistent with previous studies which suggested that shorter lag phases, lower peak times (t max ), higher P max and greater growth rate constant (k) were all good indicators of the high microbial metabolic activity in a microcalorimetric analysis (Barros et al 1997;Núñez-Regueira et al 2006b;Zheng et al 2009;Zheng et al 2007). This indicated that enhanced UV-B radiation would change the quantity and quality of soil microbes to a certain extent and prefer to inhibition.…”

Section: Soil Microbial Activitysupporting

confidence: 93%

“…Microcalorimetric technique is sensitive enough to detect very low heat rates and can provide qualitative and quantitative indicators of soil microbial activity (Barros et al 1997;Núñez-Regueira et al 2006b). This method had been applied in soil environments subjected to various factors such as land-use, heavy metal and pesticides (Barros et al 2006;Núñez-Regueira et al 2006a;Prado and Airoldi 2002;Zheng et al 2009).…”

Section: Soil Microbial Activitymentioning

confidence: 99%

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Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai–Tibet Plateau

Niu

Zhang

et al. 2014

Ecotoxicology

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The effects of enhanced UV-B radiation on abundance, community composition and the total microbial activity of soil bacteria in alpine meadow ecosystem of Qinghai-Tibet Plateau were investigated. Traditional counting and 16S rRNA gene sequencing were used to investigate the culturable bacteria and their composition in soil, meanwhile the total microbial activity was measured by microcalorimetry. The population of soil culturable bacteria was slightly reduced with the enhanced UV-B radiation in both of the two depths, 2.46 × 10(6) CFU/g in upper layer (0-10 cm), 1.44 × 10(6) CFU/g in under layer (10-20 cm), comparing with the control (2.94 × 10(6) CFU/g in upper layer, 1.65 × 10(6) CFU/g in under layer), although the difference was not statistically significant (P > 0.05). However, the bacteria diversity decreased obviously due to enhanced UV-B, the number of species for upper layer was decreased from 20 to 13, and from 16 to 13 for the lower layer. The distribution of species was also quite different between the two layers. Another obvious decrease induced by enhanced UV-B radiation was in the total soil microbial activities, which was represented by the microbial growth rate constant (k) in this study. The results indicated that the culturable bacteria community composition and the total activity of soil microbes have been considerably changed by the enhanced UV-B radiation.

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Section: Soil Microbial Activitysupporting

confidence: 93%

Section: Soil Microbial Activitysupporting

confidence: 93%

Section: Soil Microbial Activitymentioning

confidence: 99%

See 1 more Smart Citation

Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai–Tibet Plateau

Niu

Zhang

et al. 2014

Ecotoxicology

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“…Calorimetry is a powerful tool for continuously monitoring the integrated metabolism of a complex system for a prolonged period without disturbance [5]. It has been successfully applied to plant ecology [6], animal ecosystems [7], microbial communities in different soils [8,9], and microplankton in the marine environment [10,11]. In the present study, calorimetry is used in combination with the standard PFU method to investigate the metabolic and structural characteristics of the PFU microbial communities in Lake Donghu in order to determine the effects of anthropogenic stresses on the microbial activities.…”

Section: Introductionmentioning

confidence: 99%

Application of polyurethane foam units and calorimetry to microbial monitoring in Lake Donghu

et al. 2005

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“…Notably, the technique is simple, sensitive, and minimally disturbs the sample. Apart from loading the sample into the detecting unit, no additional operations are required into the reaction system (Barros et al, 1997;Critter et al, 2004a). In addition, heat signals can be continuously recorded for the same sample for a complete cycle of microbial growth and metabolic activity, which is not possible with other methods (Nuñez et al, 1994;Barros and Feijóo, 2003;Critter et al, 2004), because the acquisition of heat changes associated with all metabolic processes involved in a particular culture is continuous (Barros et al, 2012).…”

mentioning

confidence: 99%

Simple heat profiles and biogeochemical patterns for analysis the influence on soil microbial community of plastic-greenhouse and open field condition

et al. 2018

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The diversity of the soil microbial community is remarkably sensitive to different cropping techniques. To examine the effects of cultivation in a greenhouse on the soil microbial activity and diversity, we collected soil samples from vegetable crops continuously grown in a plastic greenhouse (G) and an open field (N). Heat profiling was obtained using microcalorimeter in the presence of different carbon sources. Power time curves were recorded after glucose and ammonium sulphate supplementation. The total heat release QT (Jg-1), maximum heat flow Pmax (μW) and growth rate constant k (h-1) are higher in G treatment group than the in N treatment group. On the other hand, the time (tmax) to reach the peak heat flow is lower in G treatment group compared to N treatment group, as is cell specific heat rate JQ/N (J cell-1). Sample G1 has the highest Pmax (1246.07 μW) and the lowest tmax (11.86 h); in contrast, sample N5 has the lowest Pmax (411.03 μW) and the highest tmax (21.78 h). The microbial community in the soil was estimated from phospholipid fatty acid (PLFA) biomarkers analysis and a viable count, followed by a subsequent determination of the physicochemical parameters. The total average PLFA concentration range is found as 129.76 - 142.54 nmol g-1. Both thermodynamics properties and PLFA concentration was are significantly greater in G1, G2 and G3 treatment than in the other two (N5 & N6) management systems (P < 0.05). Combination of both thermodynamic and PLFA profiling determines that the microbial population is higher in the soil from the greenhouse fertilization than from the open field cultivation. This novel approach could be applicable to a better understanding of (i) the changes in the soil microbial community in green house and open field cultivation practices, and (ii) the relationship between physicochemical properties and soil microbial community’s diversity. Moreover, this would be a notable technique for the decision maker to choose the potential cultivation method for sustainable green-food development program.

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Comparative study of the microbial activity in different soils by the microcalorimetric method

Cited by 38 publications

References 15 publications

Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai–Tibet Plateau

Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai–Tibet Plateau

Application of polyurethane foam units and calorimetry to microbial monitoring in Lake Donghu

Simple heat profiles and biogeochemical patterns for analysis the influence on soil microbial community of plastic-greenhouse and open field condition

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