Comparative study of the key enzymes and biochemical substances involved in the energy metabolism of Pacific white shrimp, Litopenaeus vannamei, with different ammonia-N tolerances

Shan, Hongwei; Geng, Zexing; Ma, Shen; Wang, Teng

doi:10.1016/j.cbpc.2019.04.001

Cited by 23 publications

(28 citation statements)

References 32 publications

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“…The present study indicated that dietary Ampithoe sp. meal supplementation can improve the ability of shrimp to resist ammonia stress, which is consistent with our previous studies (Shan et al, 2018;Shan, Geng, et al, 2019). (Chen et al, 2012;Kaleo et al, 2019).…”

Section: Effects Of Dietary Ampithoe Sp Supplementation On Ammoniasupporting

confidence: 92%

“…Our previous study indicated that dietary Ampithoe sp . supplementation could improve the energy metabolism to ensure an adequate energy supply to respond to ammonia stress (Shan, Geng, et al, 2019; Shan, Wang, et al, 2019). Energy metabolism is the basis of physiological processes in organisms; therefore, the improvement of energy metabolism may be the reason for the enhancement of glutamine synthesis and urea synthesis in L. vannamei fed Ampithoe sp.…”

Section: Discussionmentioning

confidence: 99%

“…Our preliminary studies have indicated that dietary Ampithoe sp. meal can cause changes in energy metabolism, thereby providing a better energy supply for L. vannamei to adapt to high ammonia environments (Shan et al, 2018; Shan, Geng, Ma, & Wang, 2019). On the other hand, crustaceans could adapt to a high ammonia environment by ammonia detoxification metabolism to reduce ammonia accumulation in organisms, including an increase in free diffusion, thereby allowing more ammonia to be discharged out of the body, or conversion of ammonia into the glutamine or urea stored in organisms (Chen & Chen, 1997, 2000; Wang et al, 2017; Xiao et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Changes in physiological parameters involved in glutamine and urea synthesis in Pacific white shrimp, Litopenaeus vannamei, fed Ampithoe sp. meal and exposed to ammonia stress

et al. 2020

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Two dietary formulas containing 0% (D0 group) and 33% (D33 group) Ampithoe sp. meal were fed to Litopenaeus vannamei for 42 days, and then, an ammonia stress test was performed to explore the mechanism by which dietary Ampithoe sp. meal supplementation improves the ammonia tolerance of shrimp. The changes in key enzyme activities and biochemical substances involved in glutamine and urea synthesis in shrimp under ammonia stress were investigated. The cumulative mortality in the D0 group and D33 group was 62.22% and 44.44% respectively. With the prolongation of ammonia exposure, the ammonia concentration in the haemolymph of both groups increased, but that of the D33 group was significantly lower than that of the D0 group. The glutamate dehydrogenase (GDH) activity, glutamine concentration and urea‐N concentration in the haemolymph of the D33 group were significantly higher than those of the D0 group. In the gills of the D33 group, the glutamine concentration, arginase (ARG) activity and urea‐N concentration were significantly higher than those of the D0 group. In the hepatopancreas of the D33 group, the glutamine synthetase (GS) activity, glutamine concentration and urea‐N concentration were significantly higher than those of the D0 group. In the muscle of the D33 group, the GDH activity, GS activity, glutamine concentration and ARG activity were significantly higher than those of the D0 group. Consequently, our results indicated that dietary Ampithoe sp. meal supplementation could enhance glutamine and urea synthesis, thereby reducing ammonia accumulation in organisms, resulting in the improvement of ammonia tolerance in L. vannamei.

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Section: Effects Of Dietary Ampithoe Sp Supplementation On Ammoniasupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in physiological parameters involved in glutamine and urea synthesis in Pacific white shrimp, Litopenaeus vannamei, fed Ampithoe sp. meal and exposed to ammonia stress

et al. 2020

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“…The higher haemolymph glucose in shrimp signifies stressed physiological condition along with increased energy demand (Racotta & Palacios, 1998; Shan et al., 2019). Shrimp of T240 and T260 groups contained higher haemolymph glucose as compared to other treatment groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of different levels of dietary protein on the growth performance, nutrient utilization, digestive enzymes and physiological status of white shrimp, Litopenaeus vannamei juveniles reared in inland saline water

et al. 2020

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A 60‐day feeding trial was conducted to illustrate the effect of dietary protein levels on the growth and physio‐metabolic responses of juvenile Litopenaeus vannamei reared in inland saline water (ISW). Six isoenergetic (15 MJ/kg) and isolipidic (60 g/kg) diets with graded level of crude protein viz., 240 (T240), 260 (T260), 280 (T280), 300 (T300), 320 (T320) and 340 (T340) g/kg diet were formulated. Significantly higher (p < .05) weight gain (%), specific growth rate, with lower food conversion ratio were found in T320 and T340 groups. The protein utilizing efficiency and whole‐body protein content were significantly higher (p < .05) in the T320 group. Trypsin activity increased with the increasing dietary CP level but amylase activity decreased with the increasing dietary CP level. Transaminase enzymes, haemolymph protein and haemocyanin were elevated in T320 and T340 groups. The lactate dehydrogenase and malate dehydrogenase activities were significantly higher (p < .05) in the T240 group. Shrimp of T240 group had the lowest (p < .05) glycogen and total haemocyte count with highest (p < .05) haemolymph glucose and antioxidant enzymes activities than the other groups. Based on the results, feeding 320 g CP/kg is found to be optimum for supporting maximum growth and health status of L. vannamei reared in ISW at 8 g/L salinity. The finding of the present study will help in developing a low‐cost feed for L. vannamei reared in ISW.

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“…In shrimp, previous studies have shown that ammonia secretion is closely related to ammonia tolerance [3]. Ammonia-tolerant shrimp are more able to excrete and detoxify ammonia, while also having an accelerated energy metabolism to provide sufficient energy; these adaptive strategies may increase ammonia stress tolerance [3,48]. Thus, we hypothesize that the upregulation of SLC26A6 in nitrite-tolerant shrimp increases the capacity of these shrimp to excrete and tolerate nitrite; conversely, nitrite-sensitive shrimp, which express low levels of SLC26A6, are less able to excrete and tolerate high levels of nitrite.…”

Section: Discussionmentioning

confidence: 99%

Construction of a high-density genetic map and identification of quantitative trait loci for nitrite tolerance in the Pacific white shrimp (Litopenaeus vannamei)

Zeng

Chen

Zhu

et al. 2019

Preprint

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BackgroundNitrite is a major environmental toxin in aquaculture systems disrupting multiple physiological functions in aquatic animals. Although nitrite tolerance in shrimp is closely related to successful industrial production, few genetic studies of this trait are available. ResultsIn this study, we constructed a high-density genetic map of L. vannamei with 17,242 single nucleotide polymorphism markers spanning 6,828.06 centimorgans (cM) and an average distance of 0.4 cM between adjacent markers on 44 linkage groups (LGs). Using this genetic map, we identified 2 quantitative trait loci (QTLs) involved in nitrite tolerance on two LGs explaining 8.42–10.31% of the phenotypic variance. We then sequenced the transcriptomes of the most nitrite-tolerant and the most nitrite-sensitive individuals from each of four genetically distinct L. vannamei families and found 2,002, 1,983, 1,954, and 1,867 differentially expressed genes in the four families, respectively. By integrating QTL and transcriptomics analyses, we identified a candidate gene associated with nitrite tolerance. This gene was annotated as solute carrier family 26 member 6 (SLC26A6). RNA interference (RNAi) analysis demonstrated that SLC26A6 was critical for nitrite tolerance in L. vannamei. ConclusionsThe present study increases our understanding of the molecular mechanisms underlying nitrite tolerance in shrimp, and provides a basis for molecular-marker-assisted shrimp breeding.

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Comparative study of the key enzymes and biochemical substances involved in the energy metabolism of Pacific white shrimp, Litopenaeus vannamei, with different ammonia-N tolerances

Cited by 23 publications

References 32 publications

Changes in physiological parameters involved in glutamine and urea synthesis in Pacific white shrimp, Litopenaeus vannamei, fed Ampithoe sp. meal and exposed to ammonia stress

Changes in physiological parameters involved in glutamine and urea synthesis in Pacific white shrimp, Litopenaeus vannamei, fed Ampithoe sp. meal and exposed to ammonia stress

Effects of different levels of dietary protein on the growth performance, nutrient utilization, digestive enzymes and physiological status of white shrimp, Litopenaeus vannamei juveniles reared in inland saline water

Construction of a high-density genetic map and identification of quantitative trait loci for nitrite tolerance in the Pacific white shrimp (Litopenaeus vannamei)

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