Comparative study of the immune response in mice immunized with four live attenuated strains of mumps virus by intranasal or intramuscular route

Abstract

The observation of many cases of mumps and mumps-associated CNS complications in vaccinees prompted us to perform an evaluation of the efficacy of four attenuated mumps virus (Urabe, Jeryl Lynn, Rubini and S12) vaccines. Two doses of vaccine were necessary to induce a good immunity in animals. The humoral and cell-mediated response induced in mice immunized intramuscularly or intranasally with these vaccines has been evaluated. Although the Urabe and Jeryl Lynn strains appear more immunogenic than the other strains and induce higher levels of IgG when administered intramuscularly, the S-12 strain administered intranasally induces a good IgG response. A marked specific CTL activity against mumps virus was observed in mice immunized intranasally with all the strains and, particularly, with the S12 strain. Thus, the intranasal immunization could be considered a possible alternative and efficient route of vaccination against mumps.

Genetic characterization of RS‐12 (S‐12), an Iranian isolate of mumps virus, by sequence analysis and comparative genomics of F, SH, and HN genes

“…The molecular epidemiology could yield beneficial information for development of more efficient vaccines and vaccination programs in Iran. Although the molecular epidemiology of MuV in Iran and the effectiveness of this vaccine should be reconfirmed in further studies, the present and previous results [Sassani et al, 1991;Cusi et al, 2001;Esnaashari et al, 2002;Feiterna-Sperling et al, 2005] indicate that the RS-12 vaccine may be a promising potential candidate, particularly in the Middle East.…”

“…Studies in Iran and Germany showed that RS-12 vaccine strain is immunogenic and safe [Sassani et al, 1991;Esnaashari et al, 2002;Feiterna-Sperling et al, 2005]. Further investigations indicated that the RS-12 strain could induce cytotoxic T lymphocyte (CTL) and humoral responses in mice after intranasal administration [Cusi et al, 2001]. Monkeys infected experimentally, demonstrated that RS-12 propagated in GMKC, had retained its full pathogenicity for Cynomolgus monkeys; however RS-12 subsequently adapted to growth in MRC-5 (Medical Research Council 5) cells, lost its pathogenic potential [Sassani et al, 1991].…”

Infection of Mice, Ferrets, and Rhesus Macaques with a Clinical Mumps Virus Isolate

“…immunization than by i.n. immunization (12). Splenocytes were collected 2 months after the initial infection and analyzed for cell-mediated responses by performing the enzymelinked immunosorbent spot (ELISPOT) assay to measure gamma interferon (IFN-␥) production.…”

“…Mice, as cost-efficient animals, are models for many human pathogens. However, mumps viruses are not known to replicate well or cause illness in adult mice (7,12,13). One mouse fibroblast cell-adapted mumps virus strain was reported to transiently replicate in the lungs of mice and might have circulated in the animal during infection (13).…”

“…One mouse fibroblast cell-adapted mumps virus strain was reported to transiently replicate in the lungs of mice and might have circulated in the animal during infection (13). In most studies, mice were used to evaluate immune responses induced by different attenuated strains of mumps virus (12) or to evaluate memory B cell responses after MMR vaccination (14). Ferrets, which have a respiratory tract that resembles that of humans regarding composition of sialic acids on the upper airway epithelia, provide an attractive model for the study of MuV.…”