Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress

Vuković-Gačić, Branka; Kolarević, Stoimir; Sunjog, Karolina; Tomović, Jelena; Knežević-Vukčević, Jelena; Paunović, Momír; Gačić, Zoran

doi:10.1007/s10750-013-1513-x

Cited by 25 publications

(18 citation statements)

References 48 publications

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“…Several papers (six in total) used as a model organism, the freshwater pearl mussel M. margaritifera and address aspects such as distribution and conservation status of this species in Russia (Makhrov et al, 2014;Popov & Ostrovsky, 2014), host-dependent genetic variation (Karlsson et al, 2014), the physiological response of juvenile brown trout (Salmo trutta) to encystment by the parasitic larvae (Thomas et al, 2014), captive breeding techniques (Scheder et al, 2014) and the impact of land use on M. margaritifera and its host fish S. trutta (Ö sterling & Högberg, 2014); all these works may have considerable importance for the conservation of this species and generate important information that can be used in the implementation of management measures. One paper assesses the potential use of U. pictorum and U. tumidus as model organisms for genotoxicity monitoring in freshwater environments (Vuković-Gačić et al, 2014). Three papers describe possible effects of invasive bivalves one being a review discussing the impacts from the individual to the ecosystem level and discuss some possible control strategies (Sousa et al, 2014), the second quantifies the density and biomass of massive mortalities of invasive bivalve species after extreme climatic events and decribes how this can result in a trophic subsidy to the adjacent terrestrial ecosystems (Bódis et al, 2014) and a third paper assesses the consumption of food sources and the potential competition between native (Diplodon parallelopipedon) and non-native (Corbicula fluminea) species (Marroni et al, 2014).…”

Section: The International Meeting On Biology and Conservation Of Frementioning

confidence: 99%

Biology and conservation of freshwater bivalves: past, present and future perspectives

et al. 2014

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Freshwater bivalves have been highly threatened by human activities, and recently their global decline has been causing conservational and social concern. In this paper, we review the most important research events in freshwater bivalve biology calling attention to the main scientific achievements. A great bias exists in the research effort, with much more information available for bivalve species belonging to the Unionida in comparison to other groups. The same is true for the origin of these studies, since the publishing pattern does not always correspond to the hotspots of biodiversity but is concentrated in the northern hemisphere mainly in North America, Europe and Russia, with regions such as Africa and Southeast Asia being quite understudied. We also summarize information about past, present and future perspectives concerning the most important research topics that include taxonomy, systematics, anatomy, physiology, ecology and conservation of freshwater bivalves. Finally, we introduce the articles published in this Hydrobiologia special issue related with the International Meeting on Biology and Conservation of Freshwater Bivalves held in 2012 in Bragança, Portugal.

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Section: The International Meeting On Biology and Conservation Of Frementioning

confidence: 99%

Biology and conservation of freshwater bivalves: past, present and future perspectives

et al. 2014

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“…The acclimation of mussels in controlled laboratory conditions could provide an adequate solution for obtaining the control values, i.e., the baseline DNA damage, as described in different mussel species [42,43]. In our previous study [21,22], we have shown the ability of DNA damage recovery in mussel species from the Unionidae family.…”

Section: Assessment Of Genotoxicity: In Situ Assessment and Active Bimentioning

confidence: 96%

“…Comet assay has been accepted as one of the major tools for assessing pollution-related genotoxicity in aquatic organisms [16]. It has been used in many ecogenotoxicological studies on freshwater mussels [3,6,[17][18][19][20][21][22] and has shown correlation with other genotoxicity tests such as chromosomal aberration, sister chromatid exchanges, and micronucleus assay [23]. The modified alkaline version of the comet assay, described by Singh et al [24], enables detection of both single and double DNA strand breaks, as well as alkali labile sites.…”

Section: Comet Assay (Scge)mentioning

confidence: 99%

Genotoxicological Studies of Lower Stretch of the Sava River

Vuković-Gačić

Kolarević

Sunjog

et al. 2014

The Handbook of Environmental Chemistry

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Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.

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“…A sensitive, simple and well established test for identifying a wide spectrum of DNA lesions such as single and double strand breaks and alkali-labile sites in single cells is the (Singh et al, 1988). Evaluation of the genotoxic potential of pollutants in the environment by analysing the DNA alterations in aquatic organisms has enjoyed wide acceptability, and it is a suitable method for detecting exposure in a broad range of species (Kolarević et al, 2011;Rocco et al, 2012;Sunjog et al, 2012;Vuković-Gačić et al, 2013). Because of its relevance as a very valuable fish biomarker, genotoxicity testing has been suggested to be a fundamental component of environmental risk assessment programmes ( Van der Oost et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic and genotoxic effects of perfluorododecanoic acid (PFDoA) in Japanese medaka

Ayanda

Yang

Zhang

et al. 2018

Knowl. Manag. Aquat. Ecosyst.

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-This study investigated the cytotoxic and genotoxic potential of perfluorododecanoic acid (PFDoA), a perfluorinated carboxylic chemical (PFC) that has broad applications and distribution in the environment in Japanese medaka, Oryzias latipes. Micronucleus (MN) test and Comet assay were used for the toxicity study. Three groups of fish were exposed to 0.1 mg/L, 0.5 mg/L and 2.5 mg/L concentration of the chemical for 28 days. Another group served as control. Sampling of the fish blood and liver were done after days 1, 4, 7, 14, 21 and 28 for analysis of different erythrocyte abnormalities and damage to DNA using the MN test and Comet assay respectively. Results showed that there was a significant time and concentration dependent increase (p < 0.05) in percent tail length of DNA and frequency of erythrocyte abnormalities. Nuclear abnormalities observed include micronucleus, fragmented apoptotic cells, lobed nuclei, and bean-shaped cells. Increase in induction of erythrocyte abnormalities and percent tail length of DNA peaked at days 14 and 7, respectively, after which there was a gradual decline. The results indicate that sub-chronic exposure of PFDoA to Japanese medaka caused DNA damage with a simultaneous induction of different erythrocyte abnormalities.

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Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress

Cited by 25 publications

References 48 publications

Biology and conservation of freshwater bivalves: past, present and future perspectives

Biology and conservation of freshwater bivalves: past, present and future perspectives

Genotoxicological Studies of Lower Stretch of the Sava River

Cytotoxic and genotoxic effects of perfluorododecanoic acid (PFDoA) in Japanese medaka

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