Comparative study of the flavonoids of some Verbena species cultivated in Egypt by using high-performance liquid chromatography coupled with ultraviolet spectroscopy and atmospheric pressure chemical ionization mass spectrometry

El‐Hela, Atef A.; Al‐Amier, Hussein; Ibrahim, Talaat A.

doi:10.1016/j.chroma.2010.08.025

Cited by 21 publications

(19 citation statements)

References 31 publications

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“…LC analysis of the EAF resulted in the identification of 10 phenolic compounds. The compounds were identified by their molecular ion peaks in negative mode and by comparing the reported data (Sanchez et al, 2003;Eklund et al, 2008;El-Helal et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

In vivo TNF- and IL-1 inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

Salama

El‐Hawary²,

Mousa³

et al. 2015

J. Med. Plants Res.

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A bio-guided fractionation of the defatted ethanolic extract (DEE) of the aerial parts of Trachelospermum jasminoides and its fractions: ethyl acetate fraction (EAF), chloroform fraction (CF) and n-butanol fraction (BF) were carried out, using carragenan induced rat paw edema method, to evaluate the in vivo acute anti-inflammatory potential at a given dose of 100 mg/kg body weight compared to indomethacin (20 mg/kg body weight). The EAF revealed the highest anti-inflammatory activity (76.92%) relative to the DEE and the CF (63.82 and 48.75%, respectively) at the same tested dose, while the BF was significantly inactive. The EAF and its major isolated compounds were investigated to determine the level of proinflammatory cytokines using enzyme-linked immunosorbant assay (ELISA). Seven major compounds were isolated from the EAF, identified as: trachelogenin, nor-trachelogenin and tracheloside, in addition to apigenin, luteolin, quercetin and luteolin-7-O-β-D-glucopyranoside. Identification of the isolated compounds was achieved by their physico-chemical properties and spectral analysis (1D and 2D NMR). The EAF and the isolated compounds inhibited the excessive production of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-1β. Furthermore, the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS) for the bioactive EAF was carried out to complete the phytochemical picture. The results of this study verified that the EAF and the major compounds exert their action through inhibition of TNF-α and IL-1 β.

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Section: Discussionmentioning

confidence: 99%

In vivo TNF- and IL-1 inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

Salama

El‐Hawary²,

Mousa³

et al. 2015

J. Med. Plants Res.

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“…The major classes of secondary metabolites present in Verbena officinalis are iridoids, phenylpropanoids, flavonoids, triterpenes and monoterpenes (Deepak and Handa ; El‐Hela et al . ). The iridoid verbenalin and the phenylpropanoid verbascoside are compounds with hepatoprotective (Singh et al .…”

Section: Introductionmentioning

confidence: 97%

“…The Verbenaceae family comprises approximately 100 genera of tropical and subtropical distribution, including the Verbena genus with approximately 250 species distrib-used as infusion due to therapeutic effect such as diuretic, expectorant, anti-rheumatic (Schönbichler et al 2013), antibacterial (Hernández et al 2000), antifungal (Casanova et al 2008), anti-inflammatory (Calvo et al 1998;Deepak and Handa 2000;Speroni et al 2007), analgesic (Calvo 2006) and antioxidant (Bilia et al 2008;Casanova et al 2008;Rehecho et al 2011). The major classes of secondary metabolites present in Verbena officinalis are iridoids, phenylpropanoids, flavonoids, triterpenes and monoterpenes (Deepak and Handa 2000;El-Hela et al 2010). The iridoid verbenalin and the phenylpropanoid verbascoside are compounds with hepatoprotective (Singh et al 1998) and neuroprotective effects, respectively (Sheng et al 2002), commonly identified in species of Verbena.…”

Section: Introductionmentioning

confidence: 99%

Variability and Chemical Composition of Aerials Parts of Verbena minutiflora

Soares

Pianoski

Mokochinski

et al. 2015

Journal of Food Processing and Preservation

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Verbena minutiflora (Verbenaceae) is popularly consumed as an infusion for the treatment of various diseases. However, so far there is no pharmacological or chemical validation to support its therapeutic applications. Therefore, the analysis of the chemical composition and pharmacological activities of V. minutiflora is crucial. In this study, extracts and infusions of the aerial parts of V. minutiflora (leaves, flowers and stems) were analyzed during four consecutive years. Extracts of leaves and flowers had significantly higher phenolic content and antiradical capacity than stems regardless of the year of collection. The infusions of leaves presented constant composition throughout the studied period with high levels of bioactive compounds and high antioxidant potential. Samples were analyzed by HPLC with photodiode array detector (HPLC-DAD) and by LC-ESI-MS/MS, and the potent antioxidants verbascoside and isoverbascoside (phenylpropanoids) and the iridoid hastatoside were tentatively identified in extracts and infusions of the aerial parts of V. minutiflora. PRACTICAL APPLICATIONSMedicinal plants are used since ancient times and are increasingly attracting worldwide attention as functional foods or beverages. Verbena minutiflora is popularly consumed as an infusion to reduce the levels of glucose and cholesterol in blood. Despite its popularity, so far there is no pharmaceutical or chemical validation to support their therapeutic applications. Therefore, it is extremely important to identify the main bioactive constituents of V. minutiflora in order for the plant to be safely used as a phytotherapeutic resource. All extracts showed high concentration of phenolic compounds and therefore potential antiradical capacity, especially the extracts of leaves and flowers. Despite lower amounts of phenolic and flavonoid compounds extracted by hot water, infusions showed high antiradical capacity that might be due to the presence of hastatoside, verbascoside and its isomers. This result justifies the popular intake of infusions of V. minutiflora as a source of antioxidant compounds.

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“…Verbena has longstanding use in herbalism and folk medicine, usually as an herbal tea. In fact, phytochemical investigations of Verbena species have revealed the presence of polyphenols, flavonoids, verbascosides, triterpenoids, phenylpropanoids, caffeoyl derivatives, volatile oils, and iridoids [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Allelopathic and Antimicrobial Activities of Aqueous and Methanolic Extract of Two Verbena Species (Verbena officinalis L. and Aloysia citrodora L.) Leaves

Benzarti¹

2016

Med Aromat Plants

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Antibacterial activity; Allelopathy verbena is known to have antispasmodic, antipyretic, sedative, digestive, antimicrobial, local analgesic, and antioxidant activities [13,14]. Due to these properties, lemon verbena represents a huge market potential for herbal preparation and of essential oil extraction [15]. Although plant secondary metabolites are generally associated with plant defense against herbivores and pathogens, these unique compounds can be involved in a broad array of ecological functions [16]. Phenolic compounds are one of the largest groups of secondary metabolites, consisting of four main groups divided according to the number of phenol rings and the structural elements that bind those rings, including flavonoids, phenolic acids, tannins, stilbenes and lignans [17]. Indeed, some secondary metabolites such as benzoxazinoids are regarded as natural pesticides against pathogens, microorganisms, fungi, insects, and weeds [18]. The ubiquitous use of agrochemicals has resulted in negative impacts on human health and the environment. The use of the biological activity of plant extracts against certain pests of crops remains an environmental friendly alternative. Allelopathy is a phenomenon whereby secondary metabolites synthesized by microorganisms or plants may positively

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Comparative study of the flavonoids of some Verbena species cultivated in Egypt by using high-performance liquid chromatography coupled with ultraviolet spectroscopy and atmospheric pressure chemical ionization mass spectrometry

Cited by 21 publications

References 31 publications

In vivo TNF- and IL-1 inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

In vivo TNF- and IL-1 inhibitory activity of phenolics isolated from Trachelospermum jasminoides (Lindl.) Lem

Variability and Chemical Composition of Aerials Parts of Verbena minutiflora

Allelopathic and Antimicrobial Activities of Aqueous and Methanolic Extract of Two Verbena Species (Verbena officinalis L. and Aloysia citrodora L.) Leaves

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