Comparative study of the eggshell of the fruit flies <i>Dacus oleae</i> and <i>Ceratitis capitata</i> (Diptera: Trypetidae)

Margaritis, Lukas H.

doi:10.1139/z85-324

Cited by 50 publications

(52 citation statements)

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“…The eggshell of B. oleae has a smooth chorion and is probably important in providing air to the developing embryos inside the fruit, as is known to be the case in other insect eggs (Hinton, 1981). The anterior and posterior poles that were photographed in living embryos in this study were described previously by Margaritis (1985) and Mouzaki and Margaritis (1991). The results of the present study were in agreement with their results by showing the anterior pole as inverted and cup-shaped.…”

Section: Discussionsupporting

confidence: 92%

Embryonic development of the olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae), in vivo

Genç¹

2014

Turk J Zool

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The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is a pest that infests olive fruits. The female oviposits in large green olives and larvae hatch inside the fruit, where they feed upon the fruit tissues. Larval development is completed inside the fruit. These flies cause great damage to olive production worldwide. Traditionally, insecticides have been directed against the adult stage, but the results are not efficient. This present work is a study of embryogenesis in the olive fruit fly. The external morphology of the Bactrocera oleae Rossi (Diptera: Tephritidae) egg is described from light microscopy without dechorionation. The observations were made in vivo and were photographed. The eggshell of B. oleae contains a smooth chorion with a cup-shaped anterior pole. The average length of eggs is 0.738 ± 0.01 mm and the average diameter is 0.21 ± 0.06 mm. The embryonic developmental progress is described as formation of the zygote, blastoderm and gastrulation, and organogenesis. The embryogenesis is completed within 65-70 h at 25 ± 1 °C under laboratory conditions. External egg morphology can be useful in estimating the age of B. oleae eggs for purposes such as introducing genes into embryos by germline transformation in future studies.

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Section: Discussionsupporting

confidence: 92%

Embryonic development of the olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae), in vivo

Genç¹

2014

Turk J Zool

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“…Murillo and Jirón (1994) found that A. obliqua showed greater oxygen demand by depositing their eggs in a way that the respiratory appendages remain outside of the fruit. However, Margaritis (1985) believes that species such as A. serpentina and C. capitata can oviposit in greater depth due to an air chamber in eggs, which would not require immediately atmospheric oxygen. This discussion could explain oviposition of A. fraterculus and C. capitata (Table 1), since C. capitata oviposited deeper, when compared to A. fraterculus, except for guava fruit.…”

Section: Discussionmentioning

confidence: 99%

Oviposition of fruit flies (Diptera: Tephritidae) and its relation with the pericarp of citrus fruits

et al. 2017

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Fruit flies (Diptera: Tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. This study investigated oviposition of Anastrepha fraterculus (Wiedemann, 1830) and Ceratitis capitata (Wiedemann, 1824) and its relation with the pericarp of citrus fruits. We evaluated the relationship between depth of oviposition of A. fraterculus and C. capitata and epicarp thickness of orange [Citrus sinensis (L.) Osbeck)] 'Navelina' and tangerine [C. reticulata (L.)] 'Clemenules' and the influence of fruit mesocarp of tangerine 'Clemenules' on oviposition of these species. The study was conducted under controlled conditions of temperature (25 ± 2 °C), relative humidity (70 ± 10% RH) and photophase (12 h). A. fraterculus and C. capitata laid their eggs in the flavedo region of orange 'Navelina' and between the albedo and flavedo of tangerine 'Clemenules'. When fruits with mesocarp exposed were offered, there was no oviposition by both fruit fly species. The results show that epicarp thickness of citrus fruits did not influence oviposition of A. fraterculus and C. capitata as oviposition did not occur only in the presence of the mesocarp, suggesting that other factors are involved in oviposition of these species.Keywords: Anastrepha fraterculus, Ceratitis capitata, citrus peel structure, epicarp, mesocarp.Oviposição de moscas-das-frutas (Diptera: Tephritidae) e sua relação com o pericarpo de frutos cítricos ResumoAs moscas-das-frutas (Diptera: Tephritidae) representam um risco à fruticultura mundial, especialmente na cultura dos citros, entretanto estudos biológicos demonstram que as moscas-das-frutas não estão perfeitamente adaptadas à estes hospedeiros. Este estudo investigou a oviposição de Anastrepha fraterculus (Wiedemann, 1830) e Ceratitis capitata (Wiedemann, 1824) e sua relação com o pericarpo de frutos cítricos. Foi avaliada a relação entre a profundidade de oviposição de A. fraterculus e de C. capitata e a espessura do epicarpo dos frutos de laranjeira [Citrus sinensis (L.) Osbeck)] 'Navelina' e tangerineira [C. reticulata (L.)] 'Clemenules' e a influência do mesocarpo de frutos de tangerineira 'Clemenules' na oviposição destas espécies. O estudo foi conduzido em condições controladas de temperatura (25 ± 2 °C), umidade relativa (70 ± 10%) e fotofase (12 horas). A. fraterculus e C. capitata depositaram ovos no flavedo de frutos de laranjeira 'Navelina' e entre o flavedo e o albedo de frutos de tangerineira 'Clemenules'. Quando oferecido frutos com mesocarpo exposto, não houve oviposição por ambas as espécies de mosca. Os resultados demonstram que a espessura do epicarpo de frutos cítricos não influenciou a oviposição de A. fraterculus e de C. capitata, a qual não ocorreu na presença apenas do mesocarpo, sugerindo que outros fatores estão envolvidos na oviposição por estas espécies.Palavras-chave: Anastrepha fraterculus, Ceratitis capitata, estrutura da casca de citros, epicarpo, mesocarpo.

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“…7, the exochorion (ex), the endochorionic sub-layers [outer endochorion (oe), pillars (p) and inner endochorion (ie)], the innermost chorionic layer (ICL) and the thick vitelline membrane (vm) are all highly enriched in gold particles (bPxd proteins), strongly indicating the essential biological significance and contribution of the bPxd peroxidase in the B. oleae chorion physiology. Interestingly, it was previously reported for B. oleae species, through an in situ cytochemistry approach, that, beyond the chorionic layers, the vitelline membrane also contains a strong H 2 O 2 -regulated peroxidase activity (Margaritis, 1985a). The strong immunoreactivity of the rabbit anti-rAePO polyclonal antibody with the 54 kDa chorionic component (Fig.…”

Section: Discussionmentioning

confidence: 78%

The dual role of chorion peroxidase in Bactrocera oleae chorion assembly

Konstandi¹,

Papassideri²,

Stravopodis³

et al. 2006

Int. J. Dev. Biol.

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In the present study, we reveal for the first time that the Bactrocera oleae chorion peroxidase (bPxd) participates essentially in B. oleae chorion formation and clearly represents the homologous member of Drosophila melanogaster chorion peroxidase (Pxd). Comparative sequence analysis disclosed that the bPxd cDNA semi-central region, which encodes for the putative catalytic domain of the enzyme, exhibits great homology (98%) with its Pxd counterpart. Thus, it is very likely that bPxd is highly responsible for the chorion hardening process, through protein cross-linking mediated by the formation of di-and tri-tyrosine bonds. Distinct molecular weight bPxd RNA transcripts were detected in Northern blotting analysis of total RNA extracts of adult flies (2.9 and 1.7 kb) and ovaries (2.2 kb). The ovarian-specific bPxd RNA transcript is selectively expressed in the follicle cell layer during the late stages of oogenesis 12-14, as revealed by in situ hybridization. Moreover, reverse transcription reactions confirmed the stage-specific developmental regulation of the bPxd gene, which is maximally expressed during stage 13. Western blotting with the rabbit anti-rAePO polyclonal antibody revealed three immunoreactive bands of 76, 66 and 54 kDa in crude protein extracts from adult flies, while in larva and purified chorion preparations, a unique 54 kDa band was clearly detected. Immunolocalization experiments revealed that bPxd peroxidase constitutes an essential structural chorionic component, being abundantly localized in all the successive chorionic layers and vitelline membrane as well.

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Comparative study of the eggshell of the fruit flies Dacus oleae and Ceratitis capitata (Diptera: Trypetidae)

Cited by 50 publications

References 0 publications

Embryonic development of the olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae), in vivo

Embryonic development of the olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae), in vivo

Oviposition of fruit flies (Diptera: Tephritidae) and its relation with the pericarp of citrus fruits

The dual role of chorion peroxidase in Bactrocera oleae chorion assembly

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