Comparative study of the effects of betamethasone butyrate propionate, vitamin D3 derivatives, and cyclosporine on human lymphocyte-proliferation stimulated with a hemolytic streptococci-derived superantigen

Arai, Kae; Uchiyama, Terumi; Okubo, Yukari; Tsuboi, Ryoji; Oka, Kitaro; Hirano, Toshihiko

doi:10.1016/j.ejphar.2007.06.023

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…20,21,26,27 Furthermore, it has been shown that the proliferative reactivity of PBMC against the antigens (PBMC SI) does not vary between the ConA-and SPEA-stimulated PBMC. 28 It is therefore noteworthy that, in our present study, the effects of BBP and/or calcipotriol on PBMC proliferation varied depending on whether the PBMC were stimulated by ConA or SPEA.…”

Section: Discussionmentioning

confidence: 52%

“…PBMC was chosen because the in vitro effect of immunosuppressive agents on the proliferation of PBMC is significantly correlated with the clinical efficacy of the therapeutic agents in immunological disorders . Furthermore, it has been shown that the proliferative reactivity of PBMC against the antigens (PBMC SI) does not vary between the ConA‐ and SPEA‐stimulated PBMC . It is therefore noteworthy that, in our present study, the effects of BBP and/or calcipotriol on PBMC proliferation varied depending on whether the PBMC were stimulated by ConA or SPEA.…”

Section: Discussionmentioning

confidence: 67%

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Vitamin D3 derivatives, alone or in combination with glucocorticoids, suppress streptococcal pyrogenic enterotoxin A‐stimulated proliferation of peripheral blood mononuclear cells in patients with psoriasis

Usui

Okubo

Hirano

et al. 2016

The Journal of Dermatology

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Bacterial colonization on skin or tonsil may influence the clinical response of patients with psoriasis to immunosuppressive drugs. However, few studies have investigated the effects of bacterial superantigens on therapy in these patients. Recently, combination therapy with topical glucocorticoids (GC) and vitamin D3 (VD3) appears to be more effective than GC or VD3 monotherapy for psoriasis. We evaluated the suppressive effects of betamethasone butyrate propionate (BBP), three VD3 derivatives (calcipotriol, maxacalcitol and tacalcitol), cyclosporin and BBP plus VD3, on concanavalin A (ConA)-or streptococcal pyrogenic enterotoxin A (SPEA)-stimulated proliferation of peripheral blood mononuclear cells (PBMC) obtained from 35 psoriasis patients. Drug concentrations effecting 50% inhibition concentration of ConA-or SPEA-stimulated PBMC proliferation were estimated. Cytokine levels of tumor necrosis factor-a, c-interferon, interleukin-1b, -2, -4, -5, -6, -8 -10 and -12p70 in PBMC culture supernatants were measured with bead-array procedures. Suppression of PBMC proliferation by BBP was significantly lower when PBMC were stimulated by SPEA than when stimulated by ConA. In contrast, the suppressive effects of calcipotriol and tacalcitol increased significantly when PBMC were stimulated by SPEA than when stimulated by ConA. The suppressive effect of BBP on SPEA-stimulated PBMC proliferation was improved significantly by adding 1-1000 ng/mL calcipotriol, compared with BBP alone. Cytokine levels in PBMC culture supernatants were not significantly different between ConA-and SPEA-stimulated PBMC. Calcipotriol and BBP in combination markedly suppressed SPEA-stimulated PBMC proliferation. SPEA produced by colonization of hemolytic streptococci may reduce the efficacy of BBP but not VD3 derivatives in the treatment of psoriasis.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 67%

Vitamin D3 derivatives, alone or in combination with glucocorticoids, suppress streptococcal pyrogenic enterotoxin A‐stimulated proliferation of peripheral blood mononuclear cells in patients with psoriasis

Usui

Okubo

Hirano

et al. 2016

The Journal of Dermatology

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“…Attempts have been made to evaluate the clinical utility of this type of therapy (12)(13)(14)(15) and its synergistic effects in vitro (16). In any event, decreasing the duration of combination therapy as greatly as possible and switching to VD 3 monotherapy is the most beneficial course of treatment for the patient.…”

Section: Discussionmentioning

confidence: 99%

Combination therapy using maxacalcitol and corticosteroid lotions preliminary to monotherapy with maxacalcitol lotion for scalp psoriasis

Okubo

Natsume

Usui

et al. 2012

Journal of Dermatological Treatment

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Topical treatment with betamethasone butyrate propionate lotion on 37 patients with scalp psoriasis was replaced with a combination therapy using maxacalcitol lotion (on weekdays) and BBP (on the weekends). This combination therapy was later switched to MXA monotherapy. To identify the optimum duration of the combination therapy, the patients were divided into two groups: a 4-week group and an 8-week group, which were given combination therapy and monotherapy. In both groups, the total mean scores for the skin symptoms had significantly improved in comparison with that obtained at the outset of the study (p < 0.01). In terms of overall improvement, 20.0% of the 4-week group and 72.7% of the 8-week group yielded scores reflecting moderate or greater improvement. The treatment administered to the 8-week group was significantly more effective than that given to the 4-week group at the end of the trial (p < 0.01). This study also suggests that a 4-week combination therapy is an option before switching to monotherapy, but that an 8-week therapy is preferable in severe cases.

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“…Moreover, it has been suggested that microbial superantigens induce glucocorticoid insensitivity in human PBMCs in vitro. (16,17) However, few attempts have been carried out to investigate the effect of bacterial superantigens on the efficacy of immunosuppressive drugs in treating renal transplant recipients.…”

Section: Introductionmentioning

confidence: 99%

Influence of Bacterial Superantigen TSST-1 Against the Anti-Proliferative Efficacy of Immunosuppressive Drugs and Interleukin 2 Production in Peripheral Blood Mononuclear Cells of Hemodialysis Patients and Healthy Subjects

Taira

Katsuyama

Konno

et al. 2008

Immunopharmacology and Immunotoxicology

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We investigated the influence of bacterial superantigen on the efficacies of immunosuppressive drugs on the blastogenesis of peripheral-blood mononuclear cells of 27 hemodialysis patients awaiting renal transplantation. The IC 50 values for prednisolone, methylprednisolone, cyclosporine, and tacrolimus evaluated in the superantigenstimulated cells were significantly higher than those evaluated in concanavalin A-stimulated cells (p = 0.0002-0.018). Interleukin-2 amounts produced from superantigen-stimulated cells were significantly larger than those from concanavalin A-stimulated cells (p = 0.0363). These results suggest that superantigen attenuates the suppressive efficacies of glucocorticoids and calcineurin inhibitors by stimulating lymphocytes of hemodialysis patients awaiting transplantation to overproduce interleukin-2.

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Comparative study of the effects of betamethasone butyrate propionate, vitamin D3 derivatives, and cyclosporine on human lymphocyte-proliferation stimulated with a hemolytic streptococci-derived superantigen

Cited by 8 publications

References 25 publications

Vitamin D3 derivatives, alone or in combination with glucocorticoids, suppress streptococcal pyrogenic enterotoxin A‐stimulated proliferation of peripheral blood mononuclear cells in patients with psoriasis

Vitamin D3 derivatives, alone or in combination with glucocorticoids, suppress streptococcal pyrogenic enterotoxin A‐stimulated proliferation of peripheral blood mononuclear cells in patients with psoriasis

Combination therapy using maxacalcitol and corticosteroid lotions preliminary to monotherapy with maxacalcitol lotion for scalp psoriasis

Influence of Bacterial Superantigen TSST-1 Against the Anti-Proliferative Efficacy of Immunosuppressive Drugs and Interleukin 2 Production in Peripheral Blood Mononuclear Cells of Hemodialysis Patients and Healthy Subjects

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