Comparative study of the contractile activity evoked by ATP and diadenosine tetraphosphate in isolated rat urinary bladder

Khattab, Mahmoud M.; Al‐Shabanah, Othman A.; El‐Kashef, Hassan A.

doi:10.1006/phrs.2001.0916

Cited by 6 publications

(10 citation statements)

References 38 publications

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“…Similarly, the contractile response evoked by AP 4 A in rat bladder is consistent with the reported powerful contraction induced by AP 4 A in guinea‐pig urinary bladder (Usune et al. , 1996; Khattab et al. , 2002).…”

Section: Discussionsupporting

confidence: 86%

“…A greater mechanical contractile response of the rat urinary bladder was observed in the present study as well as in the investigation of Usune et al. (1996) and Khattab et al. (2002).…”

Section: Discussionsupporting

confidence: 80%

“…The lack of potentiation may be attributed, at least in part, to the co‐modulation of NO and prostaglandins (PG). ATP‐ and AP 4 A‐induced contraction in rat urinary bladder was shown to be PG‐dependent (Khattab et al. , 2002).…”

Section: Discussionmentioning

confidence: 99%

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Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide

Khattab

Al-Hrasen

El-Hadiyah

2006

Auton Autocoid Pharmacol

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1. Both adenosine-5'-triphosphate (ATP) and diadenosine tetraphosphate (AP4A) produced a dose-dependent contraction of the isolated rat urinary bladder rings. AP(4)A dose-response curve was to the left of that of ATP, and maximum response was greater than that produced by ATP. 2. 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), the A1-purinergic receptor blocker (0.01 mm) significantly inhibited the ATP- and AP4A-induced contractions at the whole dose range. The inhibition was between 31-41%, and 15-25% for ATP and AP4A respectively. 3. Pyridoxal phosphate 6-azophenyl-2',4'-disulphonic acid (PPADS), the P2X-purinoceptor antagonist (0.01 mm) potently inhibited the bladder contractions in response to ATP and AP4A by around 75-80%. 4. The nitric oxide (NO) precursor L-arginine reduced the bladder contractile response to ATP by about 22-41% and that of AP4A to a lesser extent by around 20-32%. 5. The nitric oxide synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME, 0.1 mM), did not produce any significant effect on ATP except for a weak inhibition of about 14% at the lowest dose of ATP. The contractions in response to AP4A were only slightly reduced by L-NAME by about 20%. 6. In conclusion, the contractile response of the bladder to ATP and to the dinucleotide AP4A is mediated mainly through P2X-purinoceptors and A1-purinergic receptors. In the detrusor muscle, NO donation possesses an inhibitory effect on ATP-mediated contractility more than that produced by the dinucleotide AP4A.

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Section: Discussionsupporting

confidence: 86%

“…A greater mechanical contractile response of the rat urinary bladder was observed in the present study as well as in the investigation of Usune et al. (1996) and Khattab et al. (2002).…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide

Khattab

Al-Hrasen

El-Hadiyah

2006

Auton Autocoid Pharmacol

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“…However, it is unlikely that electrical stimulation induced a signi® cant release of acetylcholine or tachykinins as these contractions were unaOE ected by either atropine or tachykinin NK 1 and NK 2 antagonists. Thus, it is likely that contractions induced by electrical stimulation are mediated by the release of endogenous ATP, as previously documented in the mouse (Santicioli et al 1986), rat (Tong et al 1997Khattab et al 2002) and guinea-pig (Westfall et al 2000) bladder.…”

Section: Discussionmentioning

confidence: 70%

Effect of the flavonoid galangin on urinary bladder rat contractility in-vitro

Capasso

Tavares

2002

Journal of Pharmacy and Pharmacology

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Galangin is a flavanol with several biological activities. We have evaluated the effect of galangin on the contractile response elicited by electrical field stimulation (EFS) in the rat isolated urinary bladder. Galangin (10(-8)-10(-4) M) produced a concentration-dependent inhibition of the EFS contractile response without modifying the contractions produced by exogenous acetylcholine (10(-6) M). Blockade of adrenergic and cholinergic nerves with a combination of atropine (10(-6) M), phentolamine (10(-6) M) and propranolol (10(-6) M) or blockade of tachykinin NK1 and NK2 receptors with SR140333 (10(-7) M) and SR48968 (10(-6) M) did not modify the inhibitory effect of galangin. However, verapamil (10(-7) M) significantly reduced the inhibitory effect of galangin. It is concluded that the galangin inhibits EFS-induced contractions of the rat urinary bladder by acting on L-type calcium channels on presynaptic nerves.

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“…The etiology of the present patient's neurogenic bladder was not clear, but we consider that the neurogenic bladder was caused by a combination of disk hernia and theophylline. Theophylline is an antagonist of purinergic receptors, which are involved in detrusor contraction, 4 and may possibly cause dysuria.…”

Section: Discussionmentioning

confidence: 99%

Perinephric urinoma secondary to neurogenic bladder with vesicoureteral reflux: Report of an adult case

2003

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We report a case of infectious perinephric urinoma in a 73-year-old woman who had a neurogenic bladder with vesico-ureteral reflux. The patient was admitted to our emergency room with right lumbago and high fever. Ultrasounds and computed tomography demonstrated a right large perinephric cystic mass, bilateral hydronephrosis and much residual urine. Percutaneous drainage of the cystic mass was performed with an indwelling urethral catheter. The content of the mass was urine infected with Escherichia coli. Antibiotic therapy was performed successfully and we then examined the cause of the urinoma. A urodynamic study demonstrated a low-compliance small bladder and detrusor-sphincter dyssynergia. A voiding cystourethrogram revealed right grade III vesicoureteral reflux. The patient was unable to be cleared with intermittent catheterization and had an indwelling urethral catheter inserted. In 1 year, the voiding cystourethrogram showed no vesicoureteral reflux and the patient was well with no evidence of recurrent urinoma without the urethral catheter. There have been only two reported cases of urinoma caused by neurogenic bladder with vesico-ureteral reflux in children and this is the first case reported in an adult.

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Comparative study of the contractile activity evoked by ATP and diadenosine tetraphosphate in isolated rat urinary bladder

Cited by 6 publications

References 38 publications

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide

Effect of the flavonoid galangin on urinary bladder rat contractility in-vitro

Perinephric urinoma secondary to neurogenic bladder with vesicoureteral reflux: Report of an adult case

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Comparative study of the contractile activity evoked by ATP and diadenosine tetraphosphate in isolated rat urinary bladder

Cited by 6 publications

References 38 publications

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A1‐ and P2X‐purinoceptors and nitric oxide

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A1‐ and P2X‐purinoceptors and nitric oxide

Effect of the flavonoid galangin on urinary bladder rat contractility in-vitro

Perinephric urinoma secondary to neurogenic bladder with vesicoureteral reflux: Report of an adult case

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Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide

Contractile activity of ATP and diadenosine tetraphosphate on urinary bladder in the rat: role of A₁‐ and P2X‐purinoceptors and nitric oxide