Comparative study of the antioxidant activity of some thiol-containing substances

Petrov, Lubomir; Atanassova, Mila; Alexandrova, Albena

doi:10.2478/s11536-011-0132-z

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…The beneficial antioxidant properties of captopril could probably be related to the presence of the unblocked sulphydryl group in the drug molecule ( 58 ). Sulfhydryl group as a metal chelator, radical quencher, and component of the thiol/disulfide redox buffer has been recognized as a potential free radical scavenging factor ( 59 ). Interestingly, in the organism only a part of captopril binds with ACE, whereas the rest exists in an adsorbed form or as disulfides formed by the reaction with endogenous sulphydryl-containing compounds, such as glutathione or plasma proteins.…”

Section: Discussionmentioning

confidence: 99%

Angiotensin-converting enzyme inhibitors reduce oxidative stress intensity in hyperglicemic conditions in rats independently from bradykinin receptor inhibitors

et al. 2016

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AimTo investigate whether bradykinin-independent antioxidative effects of angiotensin-converting enzyme inhibitors (ACEIs) exist in acute hyperglycemia.MethodsMale Wistar rats were divided into the normoglycemic group (n = 40) and the hyperglycemic group (n = 40). Hyperglycemia was induced by a single intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight) dissolved in 0.1 mol/L citrate buffer (pH 4.5) 72 hours before sacrifice. The normoglycemic group received the same volume of citrate buffer. Each group was divided into five subgroups (n = 8): control group, captopril group, captopril + bradykinin B1 and B2 receptor antagonists group, enalapril group, and enalapril + bradykinin B1 and B2 receptor antagonists group. Captopril, enalapril, B1 and B2 receptor antagonists, or 0.15 mol/L NaCl were given at 2 and 1 hour before sacrifice. Oxidative status was determined by measuring the concentration of malondialdehyde and H2O2, and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx).ResultsIn STZ-induced hyperglycemic rats ACEIs significantly reduced H2O2 and MDA concentration, while they significantly enhanced SOD and GPx activity. The hyperglycemic group treated simultaneously with ACEIs and bradykinin B1 and B2 receptor antagonists showed a significant decrease in H2O2 concentration compared to the control hyperglycemic group.ConclusionThese results suggest the existence of additional antioxidative effect of ACEIs in hyperglycemic conditions, which is not related to the bradykinin mediation and the structure of the drug molecule.

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Section: Discussionmentioning

confidence: 99%

Angiotensin-converting enzyme inhibitors reduce oxidative stress intensity in hyperglicemic conditions in rats independently from bradykinin receptor inhibitors

et al. 2016

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“…By contrast, the addition of bME protected iNIL‐MNs from RSL3's lethality. The bME is known to have a radical scavenging activity [ 12 ]. As RSL3's lethality is independent of system x c − , this result suggests that the radical scavenging activity of bME, rather than the reduction in cystine to cysteine, rescued iNIL‐MNs from cell death.…”

Section: Resultsmentioning

confidence: 99%

Mouse embryonic stem cell‐derived motor neurons are susceptible to ferroptosis

et al. 2023

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Ferroptosis is a regulated form of cell death driven by the lethal accumulation of lipid peroxides in cell membranes. Several regulators of ferroptosis have been identified using cancer cell lines. However, the cellular pathways of ferroptosis in neurons remain poorly characterized. In this study, we used a mouse embryonic stem cell‐derived motor neuron model to investigate how motor neurons respond to ferroptosis inducers. Pharmacological and genetic inhibition of glutathione peroxidase 4 (GPx4) induced ferroptosis in motor neurons, while system xc− inhibition by erastin had no effect. RNA‐seq analysis showed that the expression levels of several genes were altered during RSL3‐induced ferroptosis. Subsequent bioinformatic analysis revealed alterations in several biological pathways during ferroptosis, including synaptogenesis and calcium signaling. Finally, we found that edaravone, an FDA‐approved drug for treating amyotrophic lateral sclerosis (ALS) disease, rescued motor neurons from RSL3‐induced ferroptosis. Our data highlight the crucial role of GPx4 in ferroptosis regulation and demonstrate that stem cell‐derived motor neuron culture is a valuable model to study ferroptosis at the single‐cell level in a neuronal context.

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Combination of Captopril with Gliclazide Decreases Vascular and Renal Complications and Improves Glycemic Control in Rats with Streptozotocin- Induced Diabetes Mellitus

Mizar

Kozman

Abo-Saif

et al. 2021

EMIDDT

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Background: The common antihypertensive angiotensin-converting enzyme (ACE) inhibitor captopril was reported to possess antioxidant and anti-inflammatory effects in different experimental models, diabetic vascular complications arises from increased vascular endothelial inflammation and oxidative stress as well as decreased nitric oxide bioavailability in the vessel walls due to poor glycemic control. Objective: This study aimed to evaluate the role of captopril and gliclazide in decreasing diabetes mellitus (DM) vascular complications caused by decreased cellular glucose uptake and impaired endothelial nitric oxide metabolism, as well as examine the effect of combination on diabetic renal complication and plasma lipid profile. Materials and methods: Adult male Wister rats received captopril (25 mg/kg/day) and/or gliclazide (10 mg/kg/day) by oral gavage daily for one month after induction of DM using streptozotocin (50 mg/kg, i.p., once). Serum glucose and insulin levels, inflammatory mediatory like TNF-α, oxidative stress biomarkers like glutathione and nitric oxide, and plasma lipid profile were measured. Besides, histopathological examination of thoracic aorta and kidney tissues, while Western blot assessed the expression of nitric oxide synthase (NOS) subtypes in the thoracic aorta. Results: Captopril significantly improved vascular architecture and oxidative stress and modulated nitric oxide synthesis via regulation of nitric oxide synthases, as well as decreased inflammation via down-regulating TNF-α, decreased systolic and diastolic blood pressure and improved serum lipid profile in diabetic rats. Gliclazide increased serum insulin and decreased serum glucose, as well as its antioxidant and anti-inflammatory effects. Discussion and conclusions: Captopril showed a promising protective effect against DM vascular complications, at least via nitric oxide modulating effect, antioxidant effect and anti-inflammatory activity that appeared in biochemical and histopathological findings, lipid profile, renal function and architecture improvements. Combining gliclazide with captopril gives an additive effect through enhanced glycemic control and increased anti-oxidant and anti-inflammatory properties above captopril alone.

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Comparative study of the antioxidant activity of some thiol-containing substances

Abstract: Abstract

Cited by 3 publications

References 20 publications

Angiotensin-converting enzyme inhibitors reduce oxidative stress intensity in hyperglicemic conditions in rats independently from bradykinin receptor inhibitors

Angiotensin-converting enzyme inhibitors reduce oxidative stress intensity in hyperglicemic conditions in rats independently from bradykinin receptor inhibitors

Mouse embryonic stem cell‐derived motor neurons are susceptible to ferroptosis

Combination of Captopril with Gliclazide Decreases Vascular and Renal Complications and Improves Glycemic Control in Rats with Streptozotocin- Induced Diabetes Mellitus

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