Comparative study of the antimicrobial activity of native and exotic plants from the Caatinga and Atlantic Forest selected through an ethnobotanical survey

Almeida, Cecília de Fátima Castelo Branco Rangel de; Cabral, Daniela Lyra de Vasconcelos; Almeida, Camila Castelo Branco Rangel de; Amorim, Elba Lúcia Cavalcanti de; Araújo, Janete Magalí de; Albuquerque, Ulysses Paulino

doi:10.3109/13880209.2011.596205

Cited by 18 publications

(10 citation statements)

References 21 publications

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“…Considering the origin of the plant material (stem-barks), the extraction method (aqueous maceration) and the data about the tannin content of these species [34], the achievement of tannins by bioguided fractionation was as expected. It is worth mentioning that, as previously reported by Almeida et al [35], Caatinga plants are exposed to high solar radiation in a semiarid environment, which favors the synthesis of phenolic derivatives, reinforcing the medicinal potential of plants from this biome.…”

Section: Discussionsupporting

confidence: 62%

Tannins Possessing Bacteriostatic Effect Impair Pseudomonas aeruginosa Adhesion and Biofilm Formation

et al. 2013

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Plants produce many compounds that are biologically active, either as part of their normal program of growth and development or in response to pathogen attack or stress. Traditionally, Anadenanthera colubrina, Commiphora leptophloeos and Myracrodruon urundeuva have been used by communities in the Brazilian Caatinga to treat several infectious diseases. The ability to impair bacterial adhesion represents an ideal strategy to combat bacterial pathogenesis, because of its importance in the early stages of the infectious process; thus, the search for anti-adherent compounds in plants is a very promising alternative. This study investigated the ability of stem-bark extracts from these three species to control the growth and prevent biofilm formation of Pseudomonas aeruginosa, an important opportunistic pathogen that adheres to surfaces and forms protective biofilms. A kinetic study (0–72 h) demonstrated that the growth of extract-treated bacteria was inhibited up to 9 h after incubation, suggesting a bacteriostatic activity. Transmission electron microscopy and fluorescence microscopy showed both viable and nonviable cells, indicating bacterial membrane damage; crystal violet assay and scanning electron microscopy demonstrated that treatment strongly inhibited biofilm formation during 6 and 24 h and that matrix production remained impaired even after growth was restored, at 24 and 48 h of incubation. Herein, we propose that the identified (condensed and hydrolyzable) tannins are able to inhibit biofilm formation via bacteriostatic properties, damaging the bacterial membrane and hindering matrix production. Our findings demonstrate the importance of this abundant class of Natural Products in higher plants against one of the most challenging issues in the hospital setting: biofilm resilience.

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Section: Discussionsupporting

confidence: 62%

Tannins Possessing Bacteriostatic Effect Impair Pseudomonas aeruginosa Adhesion and Biofilm Formation

et al. 2013

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“…Several studies have been focused on selecting plants through the ethnodirected approach [1, 9, 10], corroborating the folk use of many species that are traditionally used. Several authors also suggest that random selection should be avoided in favor of ethnodirected selection because the latter appears to be a more efficient method of selecting species for bioprospecting [7].…”

Section: Introductionmentioning

confidence: 98%

“…The search for new, natural compounds is growing, mainly due to the acquired resistance of microorganisms to commonly used drugs [1–3] and because nosocomial infections caused by these microorganisms have increasingly resulted in public health problems. Although several antibiotics are available on the market, microbial resistance to them has increased significantly, mainly due to the extensive use of drugs and the rapid genetic transfer of resistance.…”

Section: Introductionmentioning

confidence: 99%

Which Approach Is More Effective in the Selection of Plants with Antimicrobial Activity?

Silva

Santana

Saraiva

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The development of the present study was based on selections using random, direct ethnopharmacological, and indirect ethnopharmacological approaches, aiming to evaluate which method is the best for bioprospecting new antimicrobial plant drugs. A crude extract of 53 species of herbaceous plants collected in the semiarid region of Northeast Brazil was tested against 11 microorganisms. Well-agar diffusion and minimum inhibitory concentration (MIC) techniques were used. Ten extracts from direct, six from random, and three from indirect ethnopharmacological selections exhibited activities that ranged from weak to very active against the organisms tested. The strain most susceptible to the evaluated extracts was Staphylococcus aureus. The MIC analysis revealed the best result for the direct ethnopharmacological approach, considering that some species yielded extracts classified as active or moderately active (MICs between 250 and 1000 µg/mL). Furthermore, one species from this approach inhibited the growth of the three Candida strains. Thus, it was concluded that the direct ethnopharmacological approach is the most effective when selecting species for bioprospecting new plant drugs with antimicrobial activities.

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“…Caatinga medicinal plants have become the focus of intense study recently in terms of conservation and as to whether their traditional uses are supported by actual pharmacological effects or merely folklore [3, 4]. With the increasing acceptance of herbal medicine as an alternative form of health care, the screening of Caatinga medicinal plants for bioactive compounds is important and has been confirmed by the traditional uses [5–9]. …”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Phytochemical Screening ofBuchenavia tetraphylla(Aubl.) R. A. Howard (Combretaceae: Combretoideae)

Oliveira

Silva

et al. 2012

The Scientific World Journal

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This study evaluated the antimicrobial and hemolytic activities and phytochemical constituents of hydroalcoholic extract and its fractions from Buchenavia tetraphylla leaves. Cyclohexane (BTCF), ethyl acetate (BTEF), and n-butanol-soluble (BTSBF) and non-soluble (BTNBF) fractions were obtained from a liquid-liquid partition of hydroalcoholic extract (BTHE) from B. tetraphylla leaves. The hemolytic activity of active fractions was checked. The BTHE inhibited the growth of Micrococcus luteus (MIC: 0.10 mg/mL), Pseudomonas aeruginosa (MIC: 0.20 mg/mL), Mycobacterium smegmatis (MIC: 0.39 mg/mL), Proteus vulgaris, and Staphylococcus aureus (MIC: 0.78 mg/mL for both). The more active fractions were BTCF and BTBSF. BTCF showed better potential to inhibit M. luteus (0.10 mg/mL), P. aeruginosa (0.20 mg/mL), S. enteritidis (0.39 mg/mL), and S. aureus (1.56 mg/mL). BTBSF showed the best results for M. luteus (0.10 mg/mL), M. smegmatis, B. subtilis (0.39 mg/mL for both), and P. vulgaris (0.10 mg/mL). The HC50 were greater than observed MIC: 20.30, 4.70 and 2.53 mg/mL, respectively, to BTBF, BTHE and BTCF, which. The phytochemical analysis detected the presence of flavanoids, triterpene, carbohydrate, and tannin. Our work showed for the first time the broad-spread antimicrobial activity of B. tetraphylla, which has nonhemolytic action, creating a new perspective on the interesting association of traditional and scientific knowledge.

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Comparative study of the antimicrobial activity of native and exotic plants from the Caatinga and Atlantic Forest selected through an ethnobotanical survey

Abstract: Extracts from the Caatinga showed a trend of superior antimicrobial activity compared to the species from the Atlantic Forest, in terms of both inhibiting a greater variety of microorganisms and demonstrating higher activity against susceptible strains.

Cited by 18 publications

References 21 publications

Tannins Possessing Bacteriostatic Effect Impair Pseudomonas aeruginosa Adhesion and Biofilm Formation

Tannins Possessing Bacteriostatic Effect Impair Pseudomonas aeruginosa Adhesion and Biofilm Formation

Which Approach Is More Effective in the Selection of Plants with Antimicrobial Activity?

Antimicrobial Activity and Phytochemical Screening ofBuchenavia tetraphylla(Aubl.) R. A. Howard (Combretaceae: Combretoideae)

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