Comparative Study of Survival of Autologous Adipose Tissue Taken and Transplanted by Different Techniques

Nguyen, Abram; Pasyk, Krystyna A.; Bouvier, Theresa N.; Hassett, Cheryl A.; Argenta, Louis C.

doi:10.1097/00006534-199003000-00007

Cited by 322 publications

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“…In our study, the choice was for the use of 10 ml syringe for aspiration, using this option was better when compared with the use of vacuum suction device 4 . According to the author, the vacuum pressure interferes significantly in the destruction of adipocytes and the level of absorption, and at 700 mmHg the lesion reaches 15% increase in absorption after grafting, however pressures of 250, 300, 500 mmHg damages the cell less than 2% 13 .…”

Section: Resultsmentioning

confidence: 84%

“…Due to unpredictable resorption rate, fat autograft has the disadvantage of requiring a donor area and subjecting it to some local morbidity 4 . Despite the disadvantage, interest in fat grafting is large due to the nature autologous graft biocompatibility and absence of allergic reactions 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Despite the disadvantage, interest in fat grafting is large due to the nature autologous graft biocompatibility and absence of allergic reactions 4 . The availability of adipose tissue is associated with the peculiarities of each patient and indication of the donor site should therefore also be individualized.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the donor site after liposuction with a syringe in rabbits

Felzemburgh¹,

Nunes²,

Campos³

2012

Acta Cir. Bras.

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PURPOSE:To evaluate the donor site of adipocytes as well as histopathological alterations secondary to liposuction. METHODS:All animals underwent liposuction with a syringe on the right side of the back. While the left side of the back was used as control and did not undergo intervention. The 10 rabbits were divided into two groups A and B according the postoperative day which were submitted to euthanasia: 90 and 120 days. All adipose tissue from the donor site was analyzed and compared with the control macroscopic and light microscopy. Tissues were weighed and analyzed searching for histological changes and late inflammatory response to trauma such as fibrosis, fat necrosis and inflammation and macrophage infiltration. RESULTS:There was wide variation in adipose tissue volume between the experimental and the control on macroscopic analysis. The presence of histopathological changes was found in two samples at 90 days. CONCLUSIONS:There was a relationship between the presence of fibrosis with the weight and number of days after liposuction surgery in rabbits. The study show macroscopic difference between control and experiment sides in all rabbits.Key words: Adipose Tissue. Lipectomy. Back. Rabbits. RESUMO OBJETIVO:Avaliar a zona doadora de adipócitos bem como as alterações histopatológicas secundárias a lipoaspiração. MÉTODOS:Todos os animais foram submetidos à lipoaspiração com seringa no lado direito do dorso. Enquanto o lado esquerdo do dorso foi usado como controle, não sofrendo intervenção. Os 10 coelhos foram divididos em dois grupos A e B conforme o dia pós-operatório (DPO) os quais foram submetidos à eutanásia: 90 DPO e 120 DPO. Todo tecido adiposo da zona doadora foi analisado e comparados com o controle macroscopicamente e à microscopia óptica. Os tecidos foram pesados e foram analisadas as variações histológicas em busca de uma resposta inflamatória tardia ao trauma como fibrose, inflamação, necrose gordurosa e infiltrado macrofágico. RESULTADOS:Foi observada grande variação de volume de tecido adiposo entre o experimento e o controle do dorso a macroscópica e a presença de alteração histopatológica em duas amostras aos 90 dias. CONCLUSÕES:Houve relação entre a presença de fibrose com o peso e numero de dias pós-operatório na lipoaspiração de coelhos.O estudo mostrou diferença entre os lados experimento e controle em todos os coelhos.Descritores: Tecido Adiposo. Lipectomia. Dorso. Coelhos. Evaluation of the donor site after liposuction with a syringe in rabbits

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Section: Resultsmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the donor site after liposuction with a syringe in rabbits

Felzemburgh¹,

Nunes²,

Campos³

2012

Acta Cir. Bras.

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“…The published survival rates of FAG are divergent, ranging from 10 to 90% (Matsudo and Toledo, 1988;Nguyen et al, 1990;Pinski and Roenigk, 1992). The following questions remain unanswered (Goldwyn, 1988): Does the fat graft survive on the face?…”

Section: Introductionmentioning

confidence: 99%

First Use of Stereology to Quantify the Survival of Fat Autografts

Cuellar¹,

Solís²

2011

Image Anal Stereol

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It is not usual to perform quantitative analyses on surgical materials. Rather, they are evaluated clinically, through qualitative methods, and if quantitation is done, it is on a 2-dimensional basis. In this study, the long-term survival of fat autografts (FAG) in 40 subjects with facial soft tissue defects is quantified. An adipose tissue preparation from the abdomen obtained through liposuction and centrifugation is injected subcutaneously. Approximately 14 months later, the treated area is biopsied. Extensive computer-based histological analyses were performed using the stereological method in order to directly obtain three parameters: volume fraction of adipocytes in the fat tissue (V V ), density (number per volume) of adipocytes in the fat tissue (N V ), and the mean cell volume of adipocytes (VA) in each tissue sample. A set of equations based on these three quantitative parameters is produced for evaluation of the volumetric survival fraction (VSF) of FAG. The presented data evidenced a 66% survival fraction at the 14-month follow-up. In routine practice, it would be sufficient to perform this volumetric analysis on the injected and biopsied fat samples to know what fraction of the FAG has survived. This is an objective method for quantifying FAG survival and will allow a standardized comparison between different research series and authors.

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“…Unfortunately, all of these techniques have serious disadvantages, especially shrinkage and foreign body reactions [3]. Reduction of adipose volume is thought to be partly related to insufficient vascularization of grafted fat tissue [4]. Fat tissue is highly vascularized with extensive capillary networks surrounding each adipocyte, and fat tissue itself has angiogenic properties [5,6].…”

Section: Introductionmentioning

confidence: 99%

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

Hemmrich¹,

Sijpe²,

Rhodes³

et al. 2008

Journal of Surgical Research

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Background. There is a major clinical need for strategies for adequately reconstructing the soft tissue defects found after deep burns, tumor resection, or trauma. A promising solution is adipose tissue engineering with preadipocytes, stem-cell derived precursors of the adipose tissue, implanted within biomaterials. This pilot study evaluated hyaluronan gels mixed with autologous undifferentiated preadipocytes in a pig model for their potency to generate new fat.Materials and methods. Preadipocytes were isolated from intra-abdominal pig fat by collagenase digestion, plated on fibronectin-coated culture dishes in Dulbecco's Modified Eagle Medium/Ham's F12 (Biochrom, Berlin, Germany) combined with 10% pig serum, expanded, and mixed with hyaluronan gel. Two types of gels with varying degrees of amidation of the carboxyl groups were tested (HYADD3, HYADD4). Cell-loaded gels and unseeded controls were injected subcutaneously into the ears of three pigs, explanted at 6 wk, and analyzed histologically.Results. Both cell-loaded specimens were detected macroscopically. They demonstrated a slight volume effect with limited stability after 6 wk. Unloaded HYADD3 and HYADD4 controls could not be identified at the time of explantation. Histology of HYADD3 revealed islets of mature adipocytes and vessels embedded in fat tissue surrounded by gel. In contrast, no fat formation was found in HYADD4 gels when implanted in the ear.Conclusions. Histological findings demonstrate that HYADD3 is a promising gel for generating adipose tissue. Even though HYADD3 might be a potential material for the reconstruction of small tissue defects, the question remains as to whether the adipose tissue within the gel is attributable to preadipocyte maturation or ingrowth from neighboring tissue.

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Comparative Study of Survival of Autologous Adipose Tissue Taken and Transplanted by Different Techniques

Cited by 322 publications

References 0 publications

Evaluation of the donor site after liposuction with a syringe in rabbits

Evaluation of the donor site after liposuction with a syringe in rabbits

First Use of Stereology to Quantify the Survival of Fat Autografts

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

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