Comparative study of solid phase extraction techniques for isolation of leukotrienes from plasma

Salari, Hassan; Steffenrud, S.

doi:10.1016/s0378-4347(00)80697-0

Cited by 35 publications

(6 citation statements)

References 14 publications

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“…Generally, satisfying recovery of all eicosanoids was achieved in the current study (range 80 -100%), comparing favorably with previously published recoveries in the eicosanoid field (23,24,(35)(36)(37)(38).…”

Section: Solid-phase Extractionsupporting

confidence: 90%

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Kiss

Bieniek

Weißmann

et al. 1998

Analytical Biochemistry

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Section: Solid-phase Extractionsupporting

confidence: 90%

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Kiss

Bieniek

Weißmann

et al. 1998

Analytical Biochemistry

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“…After a further period of storage at -20°C for at least 2 h, proteins were eliminated by centrifugation (4000 g, for 10 min). The supernatant (2-3 ml) was diluted to less than 80% v/v methanol by addition of 8 ml of 3% aqueous acetic acid, and then submitted to SPE, as previously reported (8,9).…”

Section: Biochemical Marker Measurementŝmentioning

confidence: 99%

Leukotriene EM₄ plasma levels in adult asthmatic patients with variable disease severity

Chavis

Vyve

Chanez

et al. 1997

Allergy

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Cysteinyl leukotrienes (C-LTs) are local inflammatory mediators involved in bronchial asthma. Seventeen asthmatic patients (FEV1 ranging from 41 to 99.8% of predicted values) and 11 healthy subjects were studied. The clinical severity of asthma was assessed by the Aas score. Plasma C-LTs were measured by enzyme immunoassay (EIA) after sample purification by solid-phase extraction (SPE), to investigate whether differences may exist between asthmatic and control subjects and whether leukotriene E4 (LTE4) levels were related to the severity of disease. LT measurements showed that 87.6 +/- 1.2% was recovered as LTE4 and 9.4 +/- 1.3% as LTC4. In asthmatic subjects, LTE4 plasma levels were found to be significantly higher than those in the control group (1.073 +/- 0.133 and 0.53 +/- 0.19 ng/ml of plasma, respectively; P < 0.002). Moreover, there was a significant correlation between LTE4 plasma levels and the Aas clinical score (P < 0.005). These data suggest that plasma LTE4 levels might be used to assess the severity of asthma.

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“…Subsequently, anion‐exchange‐based sorbents were investigated as good recovery of arachidonic acid metabolites including LT derivatives have been reported for these materials . The protocol for the silica‐based mixed‐mode phase Hypersep Verify‐AX composed of a C 8 sorbent and a strong anion exchanger (SAX) was adopted from a literature method reported for the extraction of cannabinoids from blood .…”

Section: Resultsmentioning

confidence: 99%

SPE of 5-lipoxygenase metabolites and the effect of head-column field-amplified sample stacking in MEKC

Abromeit

Scriba

2013

J. Sep. Science

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The SPE of leukotrienes and eicosatetraenoic acids using anion exchange materials was compared to the classical extraction with C18 columns. A silica-based strong anion exchanger, a polymer-based weak anion exchanger, and a polymer-based mixed-mode strong anion exchanger were studied. All anion exchange materials displayed a higher recovery of the analytes with values between 70 and 90% when extracting standard solutions and analyzing by HPLC. The effect was less pronounced for the analysis of the compounds in incubations of polymorphonuclear leukocytes. Using MEKC with head-column field-amplified sample stacking for analyte quantification, much lower values of the peak areas were observed compared to the determination of the recovery of the analytes by HPLC. Using MEKC analysis, the highest values were found for the polymer-based weak anion exchange material, while values below 10% were found for the polymer-based mixed mode strong anion exchanger. This could be attributed to the presence of electrolytes in the eluates that compromised the stacking efficiency. The extent of residual electrolytes depended on the SPE protocol, resulting in large differences of the amount of analyte determined by MEKC when applying head-column field-amplified sample stacking for online analyte concentration.

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Comparative study of solid phase extraction techniques for isolation of leukotrienes from plasma

Cited by 35 publications

References 14 publications

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Leukotriene EM₄ plasma levels in adult asthmatic patients with variable disease severity

SPE of 5-lipoxygenase metabolites and the effect of head-column field-amplified sample stacking in MEKC

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Comparative study of solid phase extraction techniques for isolation of leukotrienes from plasma

Cited by 35 publications

References 14 publications

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Simultaneous Analysis of 4- and 5-Series Lipoxygenase and Cytochrome P450 Products from Different Biological Sources by Reversed-Phase High-Performance Liquid Chromatographic Technique

Leukotriene EM4 plasma levels in adult asthmatic patients with variable disease severity

SPE of 5-lipoxygenase metabolites and the effect of head-column field-amplified sample stacking in MEKC

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Leukotriene EM₄ plasma levels in adult asthmatic patients with variable disease severity