Comparative study of RP-HPLC versus TLC-spectrodensitometric methods applied for binary mixtures of fluoroquinolones and corticosteroids

Abstract

Summary.Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-spectrodensitometric methods have been developed and validated for the separation and quantitation of two binary mixtures: Ofloxacin (OFX) and dexamethasone (DXM) in eye preparation; ciprofloxacin hydrochloride (CIP) and hydrocortisone (HYD) in ear preparation. The linearity ranges of RP-HPLC methods were found to be (2.5-45 μg mL −1 ) for OFX, (2.5-50 μg mL −1 ) for DXM and (1-8 μg mL −1 ) for both CIP and HYD. The percentage recoveries/relative standard deviation (RSD) were found to be 100.36/1.38, 100.13/1.49, 99.98/0.61 and 100.28/1.27, respectively. The linearity ranges of TLC-spectrodensitometric methods were found to be (0.5-2 μg band −1 ), (0.5-3.5 μg band −1 ), (0.2-1.6 μg band −1 ), and (0.6-2 μg band −1 ) for OFX, DXM, CIP, and HYD, respectively. The percentage recoveries/RSD were found to be 99.98/1.06, 99.93/1.18, 99.74/1.27, and 99.94/1.54, respectively. A comparative study was conducted to show the advantages of the proposed methods which showed that the TLC-spectrodensitometric methods were simpler, more sensitive, and economic, while RP-HPLC methods were more precise and robust. The methods were validated in compliance with the ICH guidelines and were successfully applied for determination of the selected drugs in their laboratory-prepared mixtures and commercial dosage forms.

An integrated framework to develop an efficient valid green (EVG) HPLC method for the assessment of antimicrobial pollutants with potential threats to human health in aquatic systems

“…LC is the heart of analysis in the pharmaceutical industry owing to its accuracy, reliability, lower limits of detection, and specicity. Common LC techniques include classical reversed phase RP-HPLC, 7,8 thin-layer chromatography TLC 9,10 and chiral chromatography. 11 Solvents play the main role in achieving greenness in any LC procedure.…”

Development and validation of RP-HPLC method for quantification of trace levels of topical corticosteroids in ambiphilic cream

“…RP-HPLC (Reversed Phase High Performance Liquid Chromatography) with UV detection is often used for corticosteroids analysis, since it is selective, fast, sensitive and simple [3][4][5][6][7][8][9][10][11][12]. In order for the method to be used in quality control, its validation must be performed.…”

Analytical method development and validation for simultaneous determination of triamcinolone acetate, betamethasone valerate and clobetasol propionate in cream formulation by RP-HPLC

“…Although spectroscopic methods and TLC are simple and rapid, they have low sensitivity, accuracy, and specificity and might require sample pretreatment. Thus, they are incapable of accurate quantification on their own or require a densitometer for the quantitative aspect (Elgizawy et al, 2014). Specific methods, such as NMR spectroscopy, have also been used (McEwen et al, 2012); however, this technique requires sophisticated instruments and a special solvent.…”