Comparative study of putative 9-cis-epoxycarotenoid dioxygenase and abscisic acid accumulation in the responses of Sunki mandarin and Rangpur lime to water deficit

Neves, Diana Matos; Filho, M. A. Coelho; Bellete, Barbara Sayuri; Silva, Maria Fátima das Graças Fernandes da; Souza, Danilo Tosta; Filho, Walter dos Santos Soares; Costa, Márcio Gilberto Cardoso; Gesteira, Abelmon S.

doi:10.1007/s11033-013-2634-z

Cited by 31 publications

(36 citation statements)

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“…RL and SM rootstocks were selected based on previous studies indicating their different behaviors when subjected to drought stress2728. The reciprocal graft combinations (SM/RL and RL/SM), as well as two commercial scions were used–Valencia orange (VO) and Tahiti acid lime (TAL)–which were grafted onto SM and RL rootstocks, were used to further investigate scion/rootstock interactions.…”

Section: Discussionmentioning

confidence: 99%

“…Under severe drought, SM scions grafted onto RL exhibited leaf ABA levels approximately 2.5-fold higher than ungrafted RL. ABA has been frequently reported to be produced in roots and to translocate into leaves, especially under drought conditions2027. However, other studies have shown that ABA can also be produced in shoots and/or guard cells, protecting plants from water loss by inducing stomatal closure3637383940.…”

Section: Discussionmentioning

confidence: 99%

“…Whereas dehydration avoidance is focused on the maintenance of plant growth and productivity, dehydration tolerance is focused on plant survival, especially during prolonged drought periods16. Rangpur lime (RL) and Sunki Maravilha mandarin (SM) rootstocks exhibit different patterns of soil water uptake and protein profiles under drought conditions2728, indicating different drought survival strategies. Therefore, the present study investigated drought survival strategies of scion/rootstock reciprocal combinations between RL and SM and also combination of either RL or SM rootstocks with shoot scions of two commercial citrus varieties: Valencia orange (VO) and Tahiti acid lime (TAL).…”

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Survival strategies of citrus rootstocks subjected to drought

Santana-Vieira

Freschi

Almeida

et al. 2016

Sci Rep

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Two citrus rootstocks, Rangpur lime (RL) and Sunki Maravilha mandarin (SM), were analyzed either ungrafted or grafted with their reciprocal graft combinations or with shoot scions of two commercial citrus varieties: Valencia orange (VO) and Tahiti acid lime (TAL). All graft combinations were subjected to distinct watering regimes: well-watered, severe drought and rehydration. Growth and water relation parameters, gas exchange as well as sugar and hormone profiles were determined. Data indicated that RL adopted a dehydration avoidance strategy and maintained growth, whereas SM adopted a dehydration tolerance strategy focused on plant survival. Compared with RL, the leaves and roots of SM exhibited higher concentrations of abscisic acid and salicylic acid, which induced drought tolerance, and accumulation of carbohydrates such as trehalose and raffinose, which are important reactive oxygen species scavengers. SM rootstocks were able to transfer their survival strategy to the grafted shoot scions (RL, VO, TAL). Because of their contrasting survival strategies, RL reached the permanent wilting point more quickly than SM whereas SM recovered from prolonged droughts more efficiently than RL. This is one of the most complete studies of drought tolerance mechanisms in citrus crops and is the first to use reciprocal grafting to clarify scion/rootstock interactions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Survival strategies of citrus rootstocks subjected to drought

Santana-Vieira

Freschi

Almeida

et al. 2016

Sci Rep

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“…Rangpur lime and ‘Sunki Maravilha’ were selected because in a previous study, these cultivars have been shown to differ in their use of available soil water, ABA accumulation and expression of ABA biosynthesis genes, suggesting that they use different systems to adapt to water restriction [17]. …”

Section: Discussionmentioning

confidence: 99%

Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit

et al. 2015

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BackgroundRootstocks play a major role in the tolerance of citrus plants to water deficit by controlling and adjusting the water supply to meet the transpiration demand of the shoots. Alterations in protein abundance in citrus roots are crucial for plant adaptation to water deficit. We performed two-dimensional electrophoresis (2-DE) separation followed by LC/MS/MS to assess the proteome responses of the roots of two citrus rootstocks, Rangpur lime (Citrus limonia Osbeck) and ‘Sunki Maravilha’ (Citrus sunki) mandarin, which show contrasting tolerances to water deficits at the physiological and molecular levels.ResultsChanges in the abundance of 36 and 38 proteins in Rangpur lime and ‘Sunki Maravilha’ mandarin, respectively, were observed via LC/MS/MS in response to water deficit. Multivariate principal component analysis (PCA) of the data revealed major changes in the protein profile of ‘Sunki Maravilha’ in response to water deficit. Additionally, proteomics and systems biology analyses allowed for the general elucidation of the major mechanisms associated with the differential responses to water deficit of both varieties. The defense mechanisms of Rangpur lime included changes in the metabolism of carbohydrates and amino acids as well as in the activation of reactive oxygen species (ROS) detoxification and in the levels of proteins involved in water stress defense. In contrast, the adaptation of ‘Sunki Maravilha’ to stress was aided by the activation of DNA repair and processing proteins.ConclusionsOur study reveals that the levels of a number of proteins involved in various cellular pathways are affected during water deficit in the roots of citrus plants. The results show that acclimatization to water deficit involves specific responses in Rangpur lime and ‘Sunki Maravilha’ mandarin. This study provides insights into the effects of drought on the abundance of proteins in the roots of two varieties of citrus rootstocks. In addition, this work allows for a better understanding of the molecular basis of the response to water deficit in citrus. Further analysis is needed to elucidate the behaviors of the key target proteins involved in this response.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0416-6) contains supplementary material, which is available to authorized users.

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“…Most citrus rootstocks are intolerant to drought, and therefore, efforts have been made to improve their drought tolerance (Gong and Liu, 2013). The relationship between ABA accumulation and expression of putative NCEDs in vegetative and reproductive tissues of drought-stressed mandarins has been investigated, and a correlation between the expression of the orthologs of NCED2, 3, and 5 and the pattern of ABA accumulation has been observed in their leaves and roots (Agustí et al, 2007;Neves et al, 2013). The overexpression of citrus NCED5 was also shown to be correlated with the ABA levels in ripening fruits (Agustí et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of overexpression of citrus 9-cis-epoxycarotenoid dioxygenase 3 (CsNCED3) on the physiological response to drought stress in transgenic tobacco

Pedrosa¹,

Cidade²,

Martins³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. 9-cis-epoxycarotenoid dioxygenase (NCED) encodes a key enzyme in abscisic acid (ABA) biosynthesis. Little is known regarding the regulation of stress response by NCEDs at physiological levels. In the present study, we generated transgenic tobacco overexpressing an NCED3 ortholog from citrus (CsNCED3) and investigated its relevance in the regulation of drought stress tolerance. Wild-type (WT) and transgenic plants were grown under greenhouse conditions and subjected to drought stress for 10 days. Leaf predawn water potential (Yw leaf ), stomatal conductance (gs), net photosynthetic rate (A), transpiration rate (E), instantaneous (A/E) and intrinsic (A/gs) water use efficiency (WUE), and in situ hydrogen peroxide (H 2 O 2 ) and abscisic acid (ABA) production were determined in leaves of irrigated and drought-stressed plants. The Yw leaf decreased throughout the drought stress period in both WT and transgenic plants, but was restored after re-watering. No significant differences were observed in gs between WT and transgenic plants under normal conditions. However, the transgenic plants showed a decreased (P ≤ 0.01) gs on the 4th day of drought stress, which remained lower (P ≤ 0.001) than the WT until the end of the drought stress. The A and E levels in the transgenic plants were similar to those in WT; therefore, they exhibited increased A/gs under drought conditions. No significant differences in A, E, and gs values were observed between the WT and transgenic plants after rewatering. The transgenic plants had lower H 2 O 2 and higher ABA than the WT under drought conditions. Our results support the involvement of CsNCED3 in drought avoidance.

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Comparative study of putative 9-cis-epoxycarotenoid dioxygenase and abscisic acid accumulation in the responses of Sunki mandarin and Rangpur lime to water deficit

Cited by 31 publications

References 40 publications

Survival strategies of citrus rootstocks subjected to drought

Survival strategies of citrus rootstocks subjected to drought

Comparative study of the protein profiles of Sunki mandarin and Rangpur lime plants in response to water deficit

Effect of overexpression of citrus 9-cis-epoxycarotenoid dioxygenase 3 (CsNCED3) on the physiological response to drought stress in transgenic tobacco

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