Comparative study of protein molecular weights by size-exclusion chromatography and laser-light scattering

Dynamic mechanical properties of suspensions of micellar casein particles

“…At higher concentrations the suspensions become extremely viscous at 40 • C and ageing effects interfere if the suspensions are prepared at higher temperatures. The casein concentration was determined by measuring the absorption at 280 nm using an extinction coefficient of 0.81, which is in accordance with the data found in the literature [24,25].…”

Protein aggregation: Pathways, induction factors and analysis

“…It has been reported that basing the molecular weight solely on the elution volume has resulted in incorrectly identifying peaks as dimers. 37,91,92 It was also shown that modification of the mobile phase, such as the inclusion of arginine suppresses protein adsorption to the column matrix, 93,94 and that additional peaks may be induced due to a high salt content in the 73 Dilution, occurring during the chromatography process or sample preparation, may lead to the dissociation of reversibly formed aggregates as the concentration decreases. 73 SEC cannot only be used in combination with UV or fluorescence detectors, but also with other detectors such as light scattering detectors (e.g., multiangle laser light scattering (MALLS)), to take advantage of the light scattering technique in combination with the separation technique of soluble aggregates such as to increase accuracy in molecular weight determination.…”

“…170 The use of multiangle laser light scattering (MALLS) in combination with SEC or AF4 to determine the molecular weight of proteins and aggregates has become very popular. 37,[90][91][92]143,176 MALLS is experimentally independent of the elution order (referred to as the absolute molecular weight) and no calibration is required. 177 The known parameters required are the concentration of each elution fraction as well as the differential refractive index increment (dn/dc) to calculate the absolute value of molecular weight.…”

Structure and dynamical mechanical properties of suspensions of sodium caseinate

“…Repeated centrifugation no longer showed a turbid top layer nor a sediment. The casein concentration (C) was determined after filtration using UV absorption with an extinction coefficient of 0.81 L g −1 cm −1 [19]. The amount of protein that is eliminated by this method is less than 5%.…”