Comparative study of pit viper venoms of genera trimeresurus from Asia and bothrops from America: An immunological and isotachophoretic study

Tu, Anthony T.; Stermitz, Joseph; Ishizaki, Hiroyuki; Nonaka, Saneo

doi:10.1016/0305-0491(80)90059-0

Cited by 5 publications

(2 citation statements)

References 3 publications

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“…Three days later, another injection was made with the same amount of gilatoxin. Ten days elapsed to bleeding as this has been shown to be sufficient time for antibody production to reach high titer (Tu et al, 1980). At this time, blood was removed by ear vein puncture, red cells were removed by centrifugation, and the serum was tested by immunodiffusion against Heloderma crude venom and Heloderma major toxin.…”

Section: Methodsmentioning

confidence: 99%

Biochemical characterization of the lizard toxin gilatoxin

Hendon¹,

Tu²

1981

Biochemistry

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The Gila monster (genus Heloderma) is the only known lizard to produce and inject a venomous secretion. Little is known about the venom from these lizards, and none of the toxins have been isolated until this time. This paper reports the isolation and characterization of a major lethal toxin (gilatoxin) from the venoms of Heloderma suspectum and Heloderma horridum. Gilatoxins from both species were similar in amino acid composition, electrophoretic mobility, pI, and immunological reactivity. They are acidic proteins possessing molecular weights of 35 000-37 500 and isoelectric points of 4.25 and consist of a single polypeptide chain. Neither is antigenically related to the venoms of snakes. The toxins are devoid of phospholipase A2 activity and proteolytic, hemorrhagic, and hemolytic activities, with lethality being the only biological activity detectably expressed. The toxins appear to be unique and distinct from those of other venomous animals.

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Section: Methodsmentioning

confidence: 99%

Biochemical characterization of the lizard toxin gilatoxin

Hendon¹,

Tu²

1981

Biochemistry

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“…1980] and venoms [Tu et al, 1980;Einarsson and Moherg, 1981]. Isotachophoresis is an electrophoretic technique, in which charged components are separated in accordance with their ef fective mobilities between a leading and terminating electrolyte system.…”

Section: Introductionmentioning

confidence: 99%