Abstract:Introduction:The Institute of Technology in Immunobiology Bio-Manguinhos has been developing a kit of immunophenotyping to quantify CD4 + and CD8 + lymphocytes levels on HIV serum positive patients by flow cytometry. Currently, this kit consists of monoclonal antibodies (anti-CD3, anti-CD4, anti-CD8 and anti-CD45) produced in mice by the Center of Molecular Immunology (CIM) in Cuba. Aiming to improve the antibodies production by application of the 3R`s rule (Reduction the number of animals, Refinement of produ… Show more
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