Comparative Study of Physical Factors and Microbial Diversity of Four Man-Made Extreme Ecosystems

Imran, Ali; Akbar, Ali; Aslam, Muhammad; Ullah, Sami; Anwar, Muhammad; Punnapayak, Hunsa; Lotrakul, Pongtharin; Prasongsuk, Sehanat; Yanwisetpakdee, Benjawan; Permpornsakul, Ponlada; Rakshit, Sudip Kumar

doi:10.1007/s40011-015-0519-8

Cited by 9 publications

(7 citation statements)

References 19 publications

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“…The genetic similarity of tested isolates was further compared with previously reported ITS regions of all nineteen isolates reported from banyan, common fig, false ashoka, grapes, loquat, lychee, mandarin orange, money plant, olive, peach, pomegranate, jujube, sogo, triangle palm, spinach and mangroves ecosystem of Pakistan (Table 2). Tested isolates had 99.964% to 99.982% genetic similarity with previously reported A. alternata (Table 2) from mangroves ecosystem [28,35]. A. alternata was also isolated from high saline environment [29,36,37] but to our knowledge this is the first evidence of alternaria leaf spot of buttonwood in Uthal, Baluchistan, Pakistan.…”

Section: Plos Onesupporting

confidence: 73%

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Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale

et al. 2021

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The buttonwood (Conocarpus erectus L.) is a mangrove shrub belonging to Combretaceae family. It mostly grows on the shorelines of tropical and subtropical regions in the world. It was introduced to Lasbela University of Water, Agriculture & Marine Sciences (LUWMS), Uthal, Baluchistan as an ornamental plant as it grows well under harsh, temperate and saline conditions. During a routine survey, typical leaf spot symptoms were observed on the leaves of buttonwood plants. A disease severity scale for alternaria leaf spot of buttonwood was developed for the first time through this study. Disease severity according to the scale was 38.97%. The microscopic characterizations was accomplished for the identification of Alternaria alternata and Koch’s postulates were employed to determine the pathogenicity. For molecular identification, 650 bp internal transcribed spacer (ITS) regions (ITS1, 5.8s and ITS2) were amplified from three representative isolates (LUAWMS1, LUAWMS2 and LUAWMS3) through polymerase chain reaction (PCR). The nucleotide sequences from ITS regions of the isolates were submitted to NCBI with GenBank accession numbers MW585375, MW585376 and MW585377, respectively. The phylogenetic tree of 22 A. alternata isolates was computed and representative isolates exhibited 99.98% genetic similarity with mangroves ecosystem isolates. This study reports the incidence of alternaria leaf spot of buttonwood at LUWMS for the first time. It is suspected that the disease may spread further. Therefore, effective management strategies should be opted to halt the further spread of the disease.

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Section: Plos Onesupporting

confidence: 73%

“…Tested isolates had 99.964% to 99.982% genetic similarity with previously reported A . alternata ( Table 2 ) from mangroves ecosystem [ 28 , 35 ]. A .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale

et al. 2021

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“…be able to sustain large local population sizes even in such extreme conditions due to their high physiological and evolutionary adaptation potential linked to their small size and short generation times 11,25 . Even so, saline inland waters have so far received little attention when it comes to the diversity of microbial communities 26 compared to estuaries [27][28][29] and man-made salterns [30][31][32] . Consequently, less is known about temporary saline waters and existing studies are generally limited to specific groups like diatoms [33][34][35] or bacteria 36,37 , based on small spatial scales and a limited number of habitats 26 .…”

mentioning

confidence: 99%

Environmental changes associated with drying climate are expected to affect functional groups of pro- and microeukaryotes differently in temporary saline waters

Márton

Szabó

Vad

et al. 2023

Sci Rep

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Temporary ponds are among the most sensitive aquatic habitats to climate change. Their microbial communities have crucial roles in food webs and biogeochemical cycling, yet how their communities are assembled along environmental gradients is still understudied. This study aimed to reveal the environmental drivers of diversity (OTU-based richness, evenness, and phylogenetic diversity) and community composition from a network of saline temporary ponds, soda pans, in two consecutive spring seasons characterized by contrasting weather conditions. We used DNA-based molecular methods to investigate microbial community composition. We tested the effect of environmental variables on the diversity of prokaryotic (Bacteria, Cyanobacteria) and microeukaryotic functional groups (ciliates, heterotrophic flagellates and nanoflagellates, fungi, phytoplankton) within and across the years. Conductivity and the concentration of total suspended solids and phosphorus were the most important environmental variables affecting diversity patterns in all functional groups. Environmental conditions were harsher and they also had a stronger impact on community composition in the dry spring. Our results imply that these conditions, which are becoming more frequent with climate change, have a negative effect on microbial diversity in temporary saline ponds. This eventually might translate into community-level shifts across trophic groups with changing local conditions with implications for ecosystem functioning.

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“…In this study, 3 species of halotolerant Aspergillus and 2 species of halotolerant Penicillium were isolated from the Iko river estuary. Members of these genera have been reported by previous studies to be part of the mycobiota dominating the Iko River estuary [12, 13, 14], other estuarine ecosystems [39,40], and several saline environments [41,42,43,44,45] thus suggesting that species of Aspergillus and Penicillium are euryhaline in nature, tolerating wide ranges of salinity. These halotolerant fungi can detect high salt concentrations in the environment using the highosmolarity glycerol (HOG) signaling pathway [5].…”

Section: Discussionmentioning

confidence: 86%

Enzymatic Activities of Halotolerant and Halophilic Fungi Isolated from Iko River Estuary, South-South Nigeria

Ufot

Antia²,

Umoh³

et al. 2022

JABB

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Aim: Fungi that can tolerate unfavorable conditions are a potential source of stable bioactive metabolites that can be used for various industrial processes. Therefore this study aimed to isolate halotolerant and halophilic fungi from the Iko river estuary and screen them for three industrially important enzymes: amylase, cellulase, and mannanase. Place and Duration of Study: Department of Microbiology, Akwa Ibom State University, Nigeria, between September 2021 and August 2022. Methodology: Five sediment-dwelling fungi isolated from three locations along the Iko river estuary were assayed for their halotolerance on PDA containing different NaCl concentrations while their amylolytic, cellulolytic, and mannolytic activities were evaluated on agar plates containing 10% NaCl. The highest producer of the screened enzymes was identified using molecular techniques and further subjected to various submerged fermentation conditions to optimize the production of salt tolerant amylase, cellulase, and mannanase. Results: All isolates were halotolerant, best adapted to a salinity of 5% NaCl (w/v), and capable of secreting at least one of the three tested enzymes in 10% NaCl (w/v). Aspergillus niger isolate L2S1 had the highest enzymatic index among other isolates thus requiring optimization. The optimum production of amylase, cellulase, and mannanase was obtained at an incubation temperature of 30oC, initial pH of 6.0, and NaCl concentration of 5% (w/v). Out of the tested agro-wastes, wheat bran was most suited for the production of amylase, corncob was optimum for cellulase production, and maximum mannanase yield was obtained using copra meal. Amylase and cellulase production was optimal at 96 hours of incubation, whereas mannanase required 144 hours of fermentation to reach its maximum activity. Conclusion: Aspergillus niger isolate L2S1 was capable of utilizing different carbon sources including cheap agro-residues as substrates for the production of halostable amylase, cellulase, and mannanase.

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Comparative Study of Physical Factors and Microbial Diversity of Four Man-Made Extreme Ecosystems

Cited by 9 publications

References 19 publications

Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale

Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale

Environmental changes associated with drying climate are expected to affect functional groups of pro- and microeukaryotes differently in temporary saline waters

Enzymatic Activities of Halotolerant and Halophilic Fungi Isolated from Iko River Estuary, South-South Nigeria

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