Comparative study of peripheral blood cells in two marine fishes (Synechogobius hasta and Sebastes schlegelii): Morphological and cytochemical characterization

Han, Gaoshang; Yao, Haijing; Qiang, Lu; Chen, Xiangning; Gao, Yunzhen

doi:10.1016/j.tice.2021.101633

Cited by 6 publications

(10 citation statements)

References 46 publications

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“…Under transmission electron microscopy, erythrocytes of Paralichthys olivaceus had no organelles, which were similar to those of Gymnocyprinis eckloni(Zheng et al 2017), Schizothorax Prenanti(Fang et al 2014), Ctenopharyngodon idellus, Megalobrama amblycephala and Pelteobagrus fulvidraco(Chen et al 2019). Besides, the size of erythrocytes in Paralichthys olivaceus was smaller than that in the other two marine sh studies in our previous studies(Han et al 2021). The size of erythrocytes is inversely proportional to the metabolic activity of an organism(Smith 1925).…”

contrasting

confidence: 60%

“…The lymphocytes in the peripheral blood of Paralichthys olivaceus were the most abundant leukocyte (49.13%), the same as the previous results (Pronina et al 2020). In the work of our previous study (Han et al 2021), the per cent proportion of lymphocytes in leukocytes of Synechogobius hasta and Sebastes schlegelii reached 66.14% and 72.17%, respectively. It was proposed that variations in components of leukocytes in vertebrate species might be associated with species, age, season, and habitat conditions (Duguy 2010).…”

Section: Cytochemical Properties Of the Blood Cellsmentioning

confidence: 72%

“…According to the staining method and judging method established in the laboratory (Han et al 2021), the prepared blood smears were stained respectively. WG staining results were used for classi cation count and microscopic observation.…”

Section: Staining Methodsmentioning

confidence: 99%

“…The experimental procedure and judging standard of blood cell detection have been established in human and veterinary medicine, however, there is no uniform index in aquatic animal medicine. Fish is a lower vertebrate with too many species, and the composition and structure of peripheral blood cells vary greatly among species (Han et al 2021). It is necessary to study the basic details of peripheral blood cells in a certain sh, especially in commercial sh.…”

Section: Introductionmentioning

confidence: 99%

“…Studies have been carried out on the classi cation, and morphology of peripheral blood cells in many sh species, such as Synechogobius hasta and Sebastes schlegelii (Han et al 2021), Epinephelus akaara (Mo zur et al 2019), different freshwater shes (Ctenopharyngodon idella, Megalobrama amblycephala and Pelteobagrus fulvidraco) (Chen et al 2019) and Salvelinus fontinalis (Suljević and Mitrašinović-Brulić 2020). The cytochemical characteristics of the peripheral blood cells have also been extensively investigated in Schizothorax prenanti (Fang et al 2014), Centropomus parallelus (da Silva et al 2011), Gymnocypris eckloni (Zheng et al 2017), two Cyprinidae (Carassius auratus and Ctenopharyngodon idellus) (Zhang et al 2019) and two marine shes (Synechogobius hasta and Sebastes schlegelii) (Han et al 2021). These studies have important signi cance for enriching the blood databases of aquatic animal.…”

Section: Introductionmentioning

confidence: 99%

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Composition, structure and cytochemical characteristics of peripheral blood cells in Paralichthys olivaceus

Qiang¹,

Wang²,

Qin³

et al. 2022

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The microstructures, classification, counting and size of peripheral blood cells in Paralichthys olivaceus were observed and measured by Wright-Giemsa staining, and the cytochemical characteristics of peripheral blood cells were investigated by different cytochemical staining methods. Besides, the transmission electron microscope was used to observe the ultrastructures of different cells. The results showed that erythrocytes, thrombocytes and leukocytes constituted the peripheral blood cells in Paralichthys olivaceus, and leukocytes were made of monocytes, neutrophils and thrombocytes. More heterochromatins were found in erythrocytes, thrombocytes and neutrophils, however, monocytes had more organelles with fewer heterochromatins. As for neutrophils, endoplasmic reticulums and phagocytic vesicles were abundant. The number of erythrocytes was the highest, and the size of lymphocytes and thrombocytes was the smallest; while neutrophils were the fewest in number but the largest in size. Lymphocytes were the most abundant in leukocytes, followed by monocytes and neutrophils. The cytochemical staining results showed that all leukocytes were positive for SBB. Most of the lymphocytes were positive for PAS, and monocytes were positive for PAS, ACP and POX. As for neutrophils, ACP and POX were positive. Both monocytes and neutrophils showed positive for SBB, ACP and POX, indicating that the two kinds of cells play a vital role in phagocytosis and bactericidal action. Only lymphocytes were positive for ALP, indicating that they were important in inflammation and immune response. Paralichthys olivaceus shared the characteristics similarities in peripheral blood cells with other fishes. However, obvious differences also existed, which reflected the species speciality. These results could enrich the haematological database of bony fish and provide scientific references for researching the cell function and evaluating the health status in Paralichthys olivaceus based on haematological parameters.

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contrasting

confidence: 60%

Section: Cytochemical Properties Of the Blood Cellsmentioning

confidence: 72%

Section: Staining Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%