Comparative Study of Pathogenicity Between Aspergillus Fumigatus and Penicillium Chrysogenum in Mice

Abstract

This study was conducted to compare between two types of opportunistic fungi (Aspergillus fumigatus and Penicillium chrysogenum) in concerning their pathogenicity after intraperitoneal inoculation of mice. A total of twenty four male albino mice were used in this study which divided equally into 3 groups, The first and second groups were inoculated with 0.2ml of 1x 107spores/ml of A. fumigatus and P. chrysogenum intraperitonially respectively, while the third group was inoculated with normal saline which served as control group. All animals were monitored for 2 weeks after infection. The blood samples were collected by heart puncture after 18 days post infection to isolate of serum that used for biochemical analysis of liver and kidney functions. After that, all animals were sacrificed. Some internal organs of infected groups (liver, kidney, intestine, heart, spleen and lung) were taken to study the histopathological changes. It was found that there was severe histopathological changes in studied organs of infected mice particularly liver, kidney, spleen and intestine which corresponding with significant variation (p<0.01) in enzyme activities of liver and kidney like (Alanine Aminotransferase (ALT), Urea and Creatinine). Also, It was found that P. chrysogenum had more impact on these enzymes (15.65 ± 0.78, 135.23 ± 8.75 and 0.928 ± 0.02 respectively) than A. fumigatus (21.70 ± 1.04, 57.91 ± 5.99 and 0.587 ± 0.03 respectively). Therefore, the present study indicated that fungi present in the environment can induce severe inflammation reach to tissue damage in most vital internal organs So, further studies should be performed to determine the specific virulence factors and active components, which are responsible for pathogenesis of A. fumigatus and P. chrysogenum in spite of the fact that P. chrysogenum can produce antibiotic.

Effect of Partial Purified Lovastatin From Aspergillus Terreus A50 on Lipid Profile and Liver Enzymes in Vivo

“…The lipophilic lovastatin diminishes cholesterol synthesis and is a safe and bible treatment for the avoidance of cardiovascular illnesses. Along these lines, lovastatin is a powerful hypercholesterolemic drug used for bringing down blood cholesterol (22). Table 3 The effect of standard and partial purified lovastatin on blood lipid profile and liver enzymes in rabbits are shown in table (4) after 60 days of treatment.…”

The Effect of Fungi Accompanying cuscuta on the Germination Rates of Ocimum and cuscuta Seeds and Their Molecular Diagnosis

“…sp. (F6) in concentrations of 20 , 40 and 60 %, a significant impact on reducing the germination rate of Ocimum seeds, as the germination rate of the three concentrations reached 84.77 IOP Publishing doi:10.1088/1755-1315/1262/3/032052 7 and 83.64% sequentially compared to the comparative treatment, in which the germination rate reached 87.47% (34,35,36). And I differ significantly with the coefficients of the filtrate of the fungus Alternaria sp.…”

Hepatotoxicopathology Evaluation of Dimethylformamide (Dmf) on Male Albino Rats Livers

“…Histopathological Changes Histological samples were taken from the animals after killing, and the liver was preserved in 10% formalin as a fixative to prepare histopathological sections after the thirty days from oral administration of DMF. The paraffin sections it thickness 3 -4 𝜇m were prepared and stained with hematoxylin and eosin (H and E) for histopathological examination under light microscopy (26). Statistical analysis : Data of time recovery were analyzed as one way-ANOVA using the GLM procedure of SPSS software version 22.00 (IBM SPSS Inc., Chicago, IL, USA).…”