Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin

Darwish, Hany W.; Bakheit, Ahmed H.; Naguib, Ibrahim A.

doi:10.1155/2016/2828647

Cited by 8 publications

(5 citation statements)

References 23 publications

(19 reference statements)

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“…Calibration curve was put up for CXB by making use of amplitude difference of attained ratio spectra at 241 and 257 nm; for TMD, amplitude difference of attained ratio spectra at 220 and 237 nm against concentrations were plotted and the regression equations were computed. 12,[40][41][42][43][44][45][46]…”

Section: Ratio Difference Spectroscopic Methods (Rd)mentioning

confidence: 99%

Different Innovative UV-spectroscopic Approaches for Simultaneous Assessment of Celecoxib and Tramadol Hydrochloride in Binary Mixture

Sen,

Darji,

Sen

et al. 2023

Ind. J. Pharm. Edu. Res.

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Background and Aim: Tablets containing celecoxib and tramadol hydrochloride that are sold under the brand name Seglentis® have given approval by the "Food and Drug Administration" (FDA) for the treatment of adults suffering from acute pain. Simultaneous assessment of celecoxib and tramadol hydrochloride in synthetic mixture was performed by five new UV-spectrophotometric approaches. Materials and Methods: The quantification of proposed medication was performed using the simultaneous equation approach by measuring absorbance at 217.2 and 272.4 nm. The second approach, known as the dual wavelength method, relies on computing the absorbance difference at two different wavelengths where the other drug has same absorbance. The first derivative zero crossing approach relied on the conversion of UV-spectra into first derivative spectra and subsequently measuring the amplitude at 272.4 and 229.6 nm for celecoxib and tramadol hydrochloride, respectively. The ratio difference approach was used to measure the variation in the amplitudes of ratio spectra, and the regression equation was employed to determine the amounts of pharmaceuticals. In first derivative ratio spectra approach, the UV-spectra were transformed into their ratio spectra and first derivatives, and the first-derivative signal was gauged at 236.4 and 229.8 nm for celecoxib and tramadol hydrochloride, sequentially. Results and Discussion: All the parameters were evaluated during validation of the new methods in accordance with ICH guidelines. For both medicines, a linear response was observed in all five methods over the concentration series of 1-18 µg/mL. Method validation parameters were found to be within the permitted limits set forth by the ICH. Conclusion: Assay results were compared using Repeated Measures ANOVA, IBM SPSS, version 20.0. The findings of the statistical analysis demonstrated that none of the projected methods differed significantly from others. Thus, the anticipated approaches have the potential to be productively utilized for the simultaneous evaluation of celecoxib and tramadol hydrochloride in a combination mixture.

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Section: Ratio Difference Spectroscopic Methods (Rd)mentioning

confidence: 99%

Different Innovative UV-spectroscopic Approaches for Simultaneous Assessment of Celecoxib and Tramadol Hydrochloride in Binary Mixture

Sen,

Darji,

Sen

et al. 2023

Ind. J. Pharm. Edu. Res.

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“…Further, a calibration curve was plotted using amplitude difference against concentration. [33][34][35][36][37][38][39] Derivative Ratio Spectrum-Zero crossing Method (DRZC)…”

Section: Ratio Difference Spectroscopic Methods (Rds)mentioning

confidence: 99%

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

Sen¹,

Pandey²,

Maheshwari³

et al. 2022

Ind. J. Pharm. Edu. Res

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Background and Aim: Trijardy XR ® consisting of empagliflozin, linagliptin, and metformin hydrochloride, a fixed-dose combination (tablets) that improves glycemic control in individuals with diabetes mellitus (type 2). The present work presents four spectrophotometric methods that are quick, effortless, accurate and reproducible for the concurrent assessment of the ternary mixture. Materials and Methods: The 1 st approach works on the principle of solving established equations (simultaneous) by measuring absorbance at 224.6, 226 and 237.2 nm for empagliflozin, linagliptin and metformin hydrochloride, respectively. The second method namely ratio difference spectroscopy works by measuring the difference in amplitude at two different wavelengths in the ratio spectra. Whereas, the derivative ratio spectrum zero-crossing approach (third approach) relied on the utilization of the derivative ratio signals at zero-crossing locations. The fourth approach is the double divisor-ratio spectra derivative approach in which the first derivative of ratio spectrum was acquired and the concentrations of all 3 drugs in their combination were quantified. Results and Discussion: All the three drugs exhibited excellent linear correlation in the concentration series of 2-10 µg/ml for simultaneous equation method and 0.5-10 µg/ml for all the other methods with an exceptional correlation coefficient value. Furthermore, the projected approaches were validated as per ICH strategies and which displayed good precision, accuracy and sensitivity. Conclusion:The developed spectrophotometric approaches when compared to other analytical procedures are regarded to be more cost-effective because they do not require expensive solvents or sophisticated instruments. Therefore, the projected methods could be effectively employed for the concurrent assessment of empagliflozin, linagliptin and metformin hydrochloride in ternary mixture.

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“…Three different concentrations of the analytes (80%, 100%, and 120%) were prepared, each containing 70% of the analyte and 30% of the standard. This approach helps verify whether the method can accurately measure the true value of the analyte concentrations [19,20].…”

Section: B Accuracymentioning

confidence: 99%

Simultaneous Determination of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Forms using Ratio Subtraction Spectrophotometry

Tarigan,

Mendrofa,

Surbakti

2024

JKPK

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<p>The study focused on the simultaneous quantification of Isoniazid (INH) and Pyridoxine Hydrochloride (PRD) in tablet form, commonly used in antituberculosis treatments. Assessing the accurate concentration of both INH and PRD in tablets is vital to ensure their effectiveness, safety, and quality. Using the ratio subtraction spectrophotometry method, the study analyzed INH and PRD in Pehadoxin forte® tablets (batch no. 36057007, PT. Phapros, Indonesia), with 0.1 N HCl as the solvent. The method involved obtaining ratio absorption spectra by dividing the absorption spectra of INH and PRD, respectively, to derive zero-order spectra for each drug. Method validation parameters included linearity, accuracy, precision, Limit of Detection (LOD), and Limit of Quantification (LOQ). The results showed linearity values of 0.9985 for INH and 0.9988 for PRD. Accuracy was 98.1838% for INH and 100.0205% for PRD, while precision was 1.8769% for INH and 0.2037% for PRD. LOD and LOQ for INH were 0.8116 µg/mL and 2.7053 µg/mL, respectively, and for PRD, 1.3127 µg/mL and 4.3757 µg/mL. The levels of INH and PRD in the tablets were found to be 102.1157% and 101.3874%, aligning with the Indonesian Pharmacopoeia's standards. This methodological approach provides a reliable analytical tool for the simultaneous assessment of INH and PRD in tablets, potentially extendable to other drug combinations and formulations, thereby contributing to pharmaceutical quality control processes. </p>

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Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin

Cited by 8 publications

References 23 publications

Different Innovative UV-spectroscopic Approaches for Simultaneous Assessment of Celecoxib and Tramadol Hydrochloride in Binary Mixture

Different Innovative UV-spectroscopic Approaches for Simultaneous Assessment of Celecoxib and Tramadol Hydrochloride in Binary Mixture

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

Simultaneous Determination of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Forms using Ratio Subtraction Spectrophotometry

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