Comparative study of morphological parameters of Grand Nain banana (Musa AAA) after in vitro multiplication with growth retardants

Abstract

The incorporation of growth retardants (GR) such as ancymidol (ANC) or paclobutrazol (PBZ) in liquid culture media during multiplication stage of bananas decreases the excessive growth of stems and leaves. However, it is unknown if these chemicals have residual effect on further plant growth. To study the effect of ANC and PBZ on Grand Nain banana shoots, they were subcultured for five cycles in the multiplication stage and evaluated in elongation-rooting and acclimatization stages. Multiplication basal medium was Murashige and Skoog supplemented with 1.0 mg l )1 thiamine-HCl and 17.7 lM 6-benzylaminpurine (6-BAP), 9.75 lM ANC or 8.5 lM PBZ. Multiplied Grand Nain banana shoots were transferred to elongation-rooting stage and later on to ex-vitro conditions. The evaluation of GR residual effect and somaclonal variation on Grand Nain banana plants was determined through a comparative study of several morphological parameters in elongation-rooting and acclimatization stages. All morphological parameters evaluated in the elongation-rooting stage showed a similar performance with the exception of the pseudostem diameter of the vitroplants treated with both GR. There were significant differences for plant height in the acclimatization stage when shoots were multiplied in a medium containing ANC. The similarities of the parameters evaluated in both stages and the normal morphology recovery of vitroplants multiplied with PBZ suggest that there were no residual effects by adding PBZ to the culture media. The addition of ANC induced significant differences in plant height during the acclimatization stage.

Paclobutrazol Mediated Enhanced Multiplication of Musa Paradisiaca Linn. Cv. Poovan (Aab).

“…Moreover, the study also revealed that optimum usage of this growth retardant (1 mg L -1 ) slightly enhanced the shoot production along with considerable reduction of shoot growth to 50%. Similar findings were reported previously, where PBZ application reduced longitudinal growth of shoots in 'Grand Nain' (Albany et al, 2005), 'Williams' (Jiachuan et al, 1999). PBZ also supported enhanced bud production and here we report 17% increase in the rate of production.…”

“…Moreover, aseptic handling of overgrown culture is very difficult. A growth retardant has been used along with cytokinins to boost bud development and to reduce shoot growth in vitro (Albany et al, 2005;Daquinta et al, 2001). Enhancing the shoot production and reducing the culture growth are of great importance in mass multiplication of banana.…”

Medios de cultivo líquidos: un avance para la micropropagación comercial de zábila (Aloe barbadensis Mill.)

“…Al respecto se ha demostrado que las condiciones de los sistemas de inmersión temporal (SIT) y de los medios líquidos en agitación orbital favorecen un mayor crecimiento de tallos y hojas que no son aprovechables para la etapa de multiplicación (Albany et al, 2006(Albany et al, y 2005.…”

Aplicação de ácido giberélico (GA3) e anatomia da epiderme foliar visando à detecção de variantes somaclonais de bananeira Musa sp. Colla cv. Prata-anã (Musaceae)

“…Tal anormalidade prejudica o desenvolvimento dos cachos e dos frutos. A difi culdade em detectar esta variação, antes do repasse destas para os agricultores, tem limitado a utilização da propagação em bananeiras (Sandoval et al, 1999;Albany et al, 2005).…”

“…Tal anormalidade prejudica o desenvolvimento dos cachos e dos frutos. A difi culdade em detectar esta variação, antes do repasse destas para os agricultores, tem limitado a utilização da propagação em bananeiras (Sandoval et al, 1999;Albany et al, 2005).As causas para as diversas variações somaclonais em bananeiras ainda parecem bastante incertas. Porém, sugerese que muitas dessas anomalias podem estar relacionadas a distúrbios no metabolismo das giberelinas.…”