Comparative Study of Moringa oleifera with Moringa peregrine Seed oil using GC-MS and its Antimicrobial Activity against Helicobacter pylori

Maqbul, Muazzam Sheriff; Alshabi, Ali Mohamed; Khan, Aejaz Abdullatif; Iqubal, S. M. Shakeel; Shaikh, Ibrahim Ahmed; Mohammed, Tasneem; Habeeb, Mohammed Shafiuddin; Bokhari, Yumna Abdulmalek; Khan, Kayamkani Abedulla; Hussain, Mohammed Shahid

doi:10.13005/ojc/360318

Cited by 4 publications

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“…In the present study, PIE exhibited good antibacterial and antifungal activity, which might be one of the mechanisms responsible for its wound-healing potential. It has been previously reported that plants rich in polyphenols exhibit significant antibacterial activity [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Alsareii

Alzerwi

AlAsmari

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. When the skin and tissues within the body are injured, the healing process begins. Medicinal herbs have been used to cure wounds since time immemorial. The antimicrobial and antioxidant activity possessed by P. integrifolia may accelerate wound healing. Objectives. To assess the wound healing activity of Premna integrifolia extract (PIE) by employing in-vivo experimental animal models and an in-vitro migration scratch assay. Furthermore, to assess its cytotoxicity using the MTT assay. Methods. Wistar albino rats were used for the in vivo wound healing models. The animals were divided into four groups at random: Group I was untreated. Group II was vehicle control (ointment base). Group III was PIE ointment (5% W/W). Group IV was standard (povidone-iodine ointment) (5% W/W). The ointments were applied directly to the wounds as described above until they healed completely. The wound contraction percentage and tensile strength were calculated. The MTT test was used to determine the viability of the test extract against the fibroblast cells. The scratch assay was used in vitro to determine the wound healing potential of the test drug. P ≤ 0.05 values were considered statistically significant. Results. Premna integrifolia extract did not possess any noticeable cytotoxicity to the cell line and showed an IC50 of 185.98 μg/ml. The wound contraction potential of PIE ointment-treated animals was considerably greater ( P ≤ 0.001 ) on days 4, 8, 12, 16, and 20 when compared to the control group. The percentage of wound contraction on day 20 was 99.92% in PIE-treated animals compared to 83.23% in untreated animals. Compared to the untreated group, the duration of full epithelization was significantly ( P ≤ 0.01 ) shorter in the test group. When compared to the incision control group, the animals treated with PIE ointment had significantly higher ( P ≤ 0.001 ) tensile strength. In addition, animals given the test drug had a significant ( P ≤ 0.001 ) increase in total protein and hydroxyproline. In the in vitro scratch assay, test drug-treated cells demonstrated greater cell migration. Histology images confirmed that the test drug-treated group had epithelial tissue proliferation and keratinization. Conclusion. The current study found that Premna integrifolia improved wound healing activity both in vitro and in vivo. These findings indicate that Premna integrifolia extract has wound-healing potential and could be a viable source of nutraceuticals with wound-healing properties.

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Section: Discussionmentioning

confidence: 99%

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Alsareii

Alzerwi

AlAsmari

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Antibacterial activity of Illicium verum essential oil against MRSA clinical isolates and determination of its phyto-chemical components

Muhsinah

Maqbul

Mahnashi

et al. 2022

Journal of King Saud University - Science

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Efficacy of Phytochemical Constituents of Castor Essential oil Towards the Mucor-Mycotic Mold Cunninghamella Bertholletiae

Maqbul,

Saeed,

Dawoud

et al. 2020

JNDC

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The aim of this experiment is to study the efficacy of phytochemical constituents of Castor essential oil towards the mucor-mycotic mold Cunninghamella bertholletiae.The standard chemical analytical methods were used for the rapid study of the phytochemical constituents responsible for the antimicrobial efficacy of the procured castor essential oil. The standard antimicrobial assay technique employed to study the comparative values of the efficacy of the procured castor essential oil with that of the standard antifungal chemical agents against the clinical isolates obtained from the immune suppressed patients samples of Cunninghamella bertholletia mold mucor-mycotic infections. The best susceptibility values recorded in the standard antifungal agents against the clinical isolates of Cunninghamella bertholletiae was with Amphotericin B showing the average zone of inhibition diameter of 20.66 mm with the average MIC value of, 1.66 (µ/ml) but the antimicrobial assay results for the Castor essential oil showed better values with an average disc diffusion of 22.44mm zone of inhibition diameter with average MIC value of 1.72 µ/ml .This study has shown that the phytochemical compounds present in the Castor essential oil proves to be more an effective alternative antifungal substance towards the clinical isolates of Cunninghamella bertholletiae.

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Comparative Study of Moringa oleifera with Moringa peregrine Seed oil using GC-MS and its Antimicrobial Activity against Helicobacter pylori

Cited by 4 publications

References 0 publications

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Topical Application of Premna integrifolia Linn on Skin Wound Injury in Rats Accelerates the Wound Healing Process: Evidence from In Vitro and In Vivo Experimental Models

Antibacterial activity of Illicium verum essential oil against MRSA clinical isolates and determination of its phyto-chemical components

Efficacy of Phytochemical Constituents of Castor Essential oil Towards the Mucor-Mycotic Mold Cunninghamella Bertholletiae

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