Comparative study of microbial dechlorination of chlorinated ethenes in an aquifer and a clayey aquitard

Takeuchi, Mio; Kawabe, Yoshishige; Watanabe, Eiji; Oiwa, Toshio; Takahashi, M.; Nanba, Kenji; Kamagata, Yoichi; Hanada, Satoshi; Ohko, Yoshihisa; Komai, Takeshi

doi:10.1016/j.jconhyd.2011.01.003

Cited by 36 publications

(27 citation statements)

References 35 publications

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“…This finding coincided with a previous study in which Dehalococcoides was detected not only in sandy sediments but also in silty and clay sediments (Takeuchi et al, 2011). Dehalococcoides cells in peat sediment may obtain an electron donor and/or carbon source from the peat as suggested in a column study with peat (Mondal et al, 2016).…”

Section: Habitable Geological Environment For Dehalococcoidessupporting

confidence: 92%

“…However, the sediment samples Ac2 and Ac3, which showed a high number of vcrA, did not contain VC in the present study (Tables 2, 3). Takeuchi et al (2011) suggested that complete degradation of chlorinated ethylenes occurs in organic-rich sediment. Numbers of reductive dehalogenase genes in sediment might be affected by multiple chemical properties, such as natural organic matter, and not only VC concentration.…”

Section: Spatial Distribution Of Reductive Dehalogenase Genesmentioning

confidence: 99%

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Distribution of Dehalococcoides 16S rRNA and Dehalogenase Genes in Contaminated Sites

Yoshikawa¹,

Takeuchi²,

Zhang³

2017

ENRR

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Understanding the spatial distribution of Dehalococcoides and its reductive dehalogenase genes in sediment is important for bioremediation of sites contaminated with chlorinated ethylenes. A total of 56 sediment samples were collected from four contaminated sites in Japan, and quantified copies of Dehalococcoides 16S rRNA and reductive dehalogenase genes: tceA, bvcA, and vcrA, as well as chlorinated ethylenes. Dehalococcoides was detected from 22 sediment samples with various geological formations, textures and saturation conditions. The

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Section: Habitable Geological Environment For Dehalococcoidessupporting

confidence: 92%

Section: Spatial Distribution Of Reductive Dehalogenase Genesmentioning

confidence: 99%

Distribution of Dehalococcoides 16S rRNA and Dehalogenase Genes in Contaminated Sites

Yoshikawa¹,

Takeuchi²,

Zhang³

2017

ENRR

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“…looking at the contaminant flux from the clay till to the underlying aquifer or with regard to timeframes of a remediation using enhanced reductive dechlorination (ERD) in clay till (Lemming et al, 2010). A recent study by Takeuchi et al (2011) documented the presence of Dhc in an organic-rich clayey aquitard undergoing natural reductive dechlorination of PCE to ethene. Findings by Scheutz et al (2010) and Manoli et al (2011) in studies of ERD of chlorinated ethenes in clay tills at two Danish sites (Rugårdsvej and Sortebrovej) suggest, that Dhc are able to spread to the matrix adjacent to induced fractures and natural sand stringers resulting in bioactive zones extending up to 5 cm in the clay till matrix.…”

Section: Introductionmentioning

confidence: 99%

Identification of chlorinated solvents degradation zones in clay till by high resolution chemical, microbial and compound specific isotope analysis

Damgaard

Bjerg

Bælum

et al. 2013

Journal of Contaminant Hydrology

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a b s t r a c tThe degradation of chlorinated ethenes and ethanes in clay till was investigated at a contaminated site (Vadsby, Denmark) by high resolution sampling of intact cores combined with groundwater sampling. Over decades of contamination, bioactive zones with degradation of trichloroethene (TCE) and 1,1,1-trichloroethane (1,1,1-TCA) to 1,2-cis-dichloroethene (cis-DCE) and 1,1-dichloroethane, respectively, had developed in most of the clay till matrix. Dehalobacter dominated over Dehalococcoides (Dhc) in the clay till matrix corresponding with stagnation of sequential dechlorination at cis-DCE. Sporadically distributed bioactive zones with partial degradation to ethene were identified in the clay till matrix (thickness from 0.10 to 0.22 m). In one sub-section profile the presence of Dhc with the vcrA gene supported the occurrence of degradation of cis-DCE and VC, and in another enriched δ 13 C for TCE, cis-DCE and VC documented degradation. Highly enriched δ 13 C for 1,1,1-TCA (25‰) and cis-DCE (−4‰) suggested the occurrence of abiotic degradation in a third sub-section profile. Due to fine scale heterogeneity the identification of active degradation zones in the clay till matrix depended on high resolution subsampling of the clay till cores. The study demonstrates that an integrated approach combining chemical analysis, molecular microbial tools and compound specific isotope analysis (CSIA) was required in order to document biotic and abiotic degradations in the clay till system.

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“…The conglomerate at this site is also highly indurated (i.e., very hard), and direct push technology previously attempted at this site was unable to penetrate it. Thus, the strategy often used at lowerpermeability sites, installing a large number of wells to implement bioremediation (Damgaard et al 2013), fracturing methods (Strong et al 2004;Christiansen et al 2010;Scheutz et al 2010), or high-resolution sampling (Takeuchi et al 2011), is impractical because direct push technology cannot be used at this site.…”

Section: Introductionmentioning

confidence: 99%

A Long-Term Field Study of In Situ Bioremediation in a Fractured Conglomerate Trichloroethene Source Zone

Verce

Madrid

Gregory

et al. 2015

Bioremediation Journal

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An 8-year bioremediation field study was conducted in a trichloroethene (TCE)-contaminated, highly indurated (i.e., hard), rechargelimited (i.e., contains little water) conglomerate where common remediation strategies, such as groundwater recirculation and direct push installation of a large well network, could not be used. A tracer test using isotopically distinct water from the Hetch Hetchy Reservoir indicated that remediation fluids mainly flowed through fractures and sand lenses in the conglomerate. This was confirmed during in situ bioremediation of the site, in which Dehalococcoides (from a bioaugmentation culture) and volatile fatty acids (from injection of lactate) were the most accurate indicators of transport between wells. Some contaminants were also displaced out of the area due to injection of tracer water. Despite these difficulties, dissolved contaminant mass decreased by an estimated 80% by the end of the test, reaching the lowest values ever recorded at this site. Furthermore, the persistence of ethene 4 years after bioaugmentation suggests that the dechlorinating capacity of the remaining microbial community is comparable to the matrix diffusion of TCE into the dissolved phase.

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Comparative study of microbial dechlorination of chlorinated ethenes in an aquifer and a clayey aquitard

Cited by 36 publications

References 35 publications

Distribution of Dehalococcoides 16S rRNA and Dehalogenase Genes in Contaminated Sites

Distribution of Dehalococcoides 16S rRNA and Dehalogenase Genes in Contaminated Sites

Identification of chlorinated solvents degradation zones in clay till by high resolution chemical, microbial and compound specific isotope analysis

A Long-Term Field Study of In Situ Bioremediation in a Fractured Conglomerate Trichloroethene Source Zone

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