Comparative study of macrophages in naked mole rats and ICR mice

Abstract

The domestic and foreign scholars have studied naked mole rats more focused on the respect such as its long life, resistant to low oxygen, little spontaneous tumor, but the study of the immune system is little. In this study, we compared the anatomy and tissue morphology of NMR and ICR mouse spleens and found that the gross appearance of the NNMR spleen differed from ICR. There were more macrophages in NNMR spleens than in ICR spleens. Furthermore, we focused on the differences of macrophages. We compared their phagocytic capabilities and the data showed that NNMR macrophages are more phagocytic than ICR mouse macrophages. We also used polyI:C and LPS to stimulate the NMR and ICR macrophages and then measured the immune response as expression of certain TLR signaling molecules. After stimulation, there was a lower increase in apoptosis of NMR macrophages than ICR macrophages and a non-significant increased expression of TLRs in NMR macrophages than in ICR macrophages. In contrast, NF-κB proteins increased more significantly in NMR’s than in ICR’s and the expression of downstream cytokines in NMR macrophages also increased more than in ICR macrophages. Based on these results, we hypothesize that in addition to being able to eat foreign matter, NMR macrophages can activate the TLRs, start the NF-κB and produce a large number of cytokines to enhance immune response, so as to protect the body from outside interference when the virus or bacteria invading.

Identification of cross‐reactive antibodies for the detection of lymphocytes, myeloid cells and haematopoietic precursors in the naked mole rat

“…Data for the mouse (averaged for CB17, C57BL/6 and BALB/c strains) and humans are taken from the literature sources [19][20][21][22]. The chamber bedding consists The difference is considered statistically significant if p < 0.05.…”

“…[23] [19,20,22] Spleen Lower frequency of lymphocytes and higher frequency of granulocytes compared to mouse spleen. High expression of CD14 on myeloid cells.…”

The Naked Mole Rat: A Unique Example of Positive Oxidative Stress

“…Studies of the naked mole rat immune system are limited. A recent study demonstrated that the naked mole rat spleen has significant anatomical and morphological differences compared to mouse spleen with higher abundance of macrophages better equipped for phagocytosis [89]. It is speculated that a higher abundance of macrophages with greater phagocytic capability and greater induction of proinflammatory cytokines may result in a strong immune system.…”

Suppressed LPS‐mediated TLR4 signaling in the plateau zokor (Eospalax baileyi) compared to the bamboo rat (Rhizomys pruinosus) and rat (Rattus norvegicus)

“…In both cases, the immune response was greater in wild mice than in laboratory mice (Abolins et al, 2017; Abolins et al, 2018; Abolins, Pocock, Hafalla, Riley, & Viney, 2011; Lochmiller, Vestey, & Mcmurry, 1991). Similarly, in a study of the innate immunity of the naked mole‐rat ( Heterocephalus glaber ), an underground rodent, macrophages were immunized with liposaccharide (LPS) and poly(I:C), and the effects were compared with those in mice; naked mole‐rats initiated an innate immune response faster than laboratory mice (Cheng et al, 2017). However, there has been no similar study on an underground rodent unique to the Tibetan Plateau.…”