Comparative study of macrophage response in mice after DNA immunization and infection with herpes simplex virus type 1

Abstract

Functional activity of macrophages and intensity of T cell immune response in mice were studied after intravaginal and intraperitoneal infection with herpes simplex virus type 1 and DNA vaccination in combination with adjuvant treatment (recombinant granulocyte-macrophage colony-stimulating factor and glucosaminylmuramyl dipeptide). DNA vaccination induced a virus-specific T cell immune response with no macrophagic inflammatory reaction. Infection with herpes simplex virus type 1 was accompanied by sustained inflammation, but not by the T cell immune response.

Functional activity of peritoneal macrophages from sensitive and resistant mouse strains during intravaginal infection with herpes simplex virus type 2 and mucosal vaccination

“…The results were expressed in optical density units per mg protein. Cathepsin D activity (µg tyrosine per mg protein) and protein concentration (Lowry method) were measured in lysates of the cell monolayer to estimate lysosomal enzyme activity [1]. Lymphocyte from regional lymph nodes were washed 2 times and resuspended in the culture medium to perform MMIR.…”

“…The tip of automatic pipette was filled with 5 µl cell suspension (5×10 6 cells/ml) and placed in a special stand. The stand was mounted in a 96-well plate prefilled with the culture medium (100 µl per well) [1]. Inactivated HSV2 in a concentration of 10 PFU was added to several wells as the antigen.…”