Comparative Study of in Vitro Free Radical Scavenging Activity of Different Leaf Extracts of Ixora Coccinea L.

Abstract

In this study free radical scavenging activity of chloroform, methanolic and aqueous leaf extracts of Ixora coccinea L. (Rubiaceae) were determined and compared by in vitro assay models such as DPPH free radical, nitric oxide radical, hydroxyl radical scavenging assay, reductive ability and total antioxidant capacity. Ascorbic acid was used as reference standard. IC 50 of all the exracts in all the method were determined. Among all the three extracts, methanolic extract have shown better scavenging and antioxidant property compared to other extracts in all the assay methods evaluated thereby leading it into the modern system of medicine in future.

Comparative Study of Thin-Layer Chromatography Bioautography and Antioxidant Activities of Different Parts of Clitoria Ternatea (Fabaceae)

“…Overproduction of these free radicals leads to severe pathological conditions [1] and damage of cellular components by covalent binding and lipid peroxidation leading to tissue injury and in turn to the development and/or progression of various conditions or diseases such as aging, carcinogenesis, physical injury, infection and cardio vascular, liver cirrhosis, diabetes, nephrotoxicity, and degenerative diseases [2,3]. These are potentially very toxic to cells and in the body are usually generated by common metabolic processes [4][5][6]. Organisms possess antioxidants and antioxidant enzymes to combat these free radicals but external supplementation of antioxidants is equally needed [1], which scavenge or remove these free radicals and thereby terminate the chain reactions and inhibit different oxidative reactions are termed as antioxidants.…”