Comparative study of IgG binding to megakaryocytes in immune and myelodysplastic thrombocytopenic patients

Elzaeem, Doaa I; Sharkawi, Esmat A El; Zaki, Eman M.; Ghobrial, Ayman G; El-Fatah, Aliaa S Abd; El-Hamed, Waleed M Abd

doi:10.1007/s00277-021-04556-2

Cited by 3 publications

(4 citation statements)

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Section: Resultsmentioning

confidence: 99%

“…While formerly considered a form of pre-leukemia, MDS is regarded as a malignant myeloid hematopoietic condition often characterized by certain genetic mutations and morphologic alterations. Most patients have the complications of low platelet count in the circulation and abnormalities of megakaryocytes in the bone marrow 18,19 and these 2 features are associated with the progression and prognosis of the disease 20,21 . Some recent case reports have reported morphologic alterations of megakaryocytes in bone marrow from MDS patients 22,23 …”

Section: Resultsmentioning

confidence: 99%

“…Most patients have the complications of low platelet count in the circulation and abnormalities of megakaryocytes in the bone marrow 18,19 and these 2 features are associated with the progression and prognosis of the disease. 20,21 Some recent case reports have reported morphologic alterations of megakaryocytes in bone marrow from MDS patients. 22,23 In the present study, megakaryocytes from MDS patients were characterized by an asymmetric development of nucleus and cytoplasm, and aberrant distribution of cytoplasmic organelles in 5 out of 6 cases; in the 1 remaining case, megakaryocytes showed poorly differentiated features.…”

Section: Myelodysplastic Syndromementioning

confidence: 99%

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Ultrastructural alterations of megakaryocytes in thrombocytopenia: A review of 43 cases

Eyden

Dong

et al. 2021

Blood Science

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Thrombocytopenia is a frequent occurrence in a variety of hematopoietic diseases; however, the details of the mechanism leading to low platelet count remain elusive. Megakaryocytes are a series of progenitor cells responsible for the production of platelets. Alterations in megakaryocytes in the bone marrow are a causative factor resulting in thrombocytopenia in varied diseases. Based on ultrastructural analysis of incidentally encountered megakaryocytes in 43 patients with blood diseases marked by low platelet counts, electron micrographs demonstrated that aberrant megakaryocytes predominated in idiopathic thrombocytopenic purpura, aplastic anemia, and myelodysplastic syndrome; autophagy, apoptosis, and cellular damage in megakaryocytes were a prominent feature in aplastic anemia. On the other hand, poorly differentiated megakaryocytes predominated in acute megakaryoblastic leukemia (AMKL) although damaged megakaryocytes were seen in non-AMKL acute leukemia. This paper documents the ultrastructural alterations of megakaryocytes associated with thrombocytopenia and reveals distinctive features for particular blood diseases. A comment is made on future avenues of research emphasizing membrane fusion proteins.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Myelodysplastic Syndromementioning

confidence: 99%

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Ultrastructural alterations of megakaryocytes in thrombocytopenia: A review of 43 cases

Eyden

Dong

et al. 2021

Blood Science

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“…There was a clear negative correlation between PA-IgG levels and PLT ( 27 ). Megakaryocytes in the bone marrow of patients with ITP exhibit strong binding to IgG ( 28 ). IgG and IgM antiplatelet antibodies can be used to diagnose ITP ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota alterations in children and their relationship with primary immune thrombocytopenia

Zhang

et al. 2023

Front. Pediatr.

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IntroductionGut microbiota reportedly play a critical role in some autoimmune diseases by maintaining immune homeostasis. Only a few studies have examined the correlation between gut microbiota and the onset of primary immune thrombocytopenia (ITP), especially in children. The purpose of this study was to investigate changes in the composition and diversity of the fecal microbiota of children with ITP, as well as the correlation between such microbiota and the onset of ITP.MethodsTwenty-five children newly diagnosed with ITP and 16 healthy volunteers (controls) were selected for the study. Fresh stool samples were collected to identify changes in the composition and diversity of gut microbiota as well as for potential correlation analysis.ResultsIn ITP patients, the phyla that were most frequently encountered were Firmicutes (54.3%), followed by Actinobacteria (19.79%), Bacteriodetes (16.06%), and Proteobacteria (8.75%). The phyla that were predominantly found in the controls were, Firmicutes (45.84%), Actinobacteria (40.15%), Bacteriodetes (3.42%), and Proteobacteria (10.23%). Compared with those of the controls, the proportions of Firmicutes and Bacteriodetes in the gut microbiota of ITP patients were increased while the proportions of Actinobacteria and Proteobacteria were decreased. Furthermore, gut microbiota in ITP patients varied by age group, showed specific changes in diversity, and were correlated with antiplatelet antibodies. IgG levels were significantly positively correlated with Bacteroides (P<0.01).ConclusionsThe gut microbiota of children with ITP are imbalanced, as shown by the increase in Bacteroidetes, which was positively correlated with IgG. Thus gut microbiota may contribute to ITP pathogenesis via IgG.Clinical Trial RegistrationThe clinical trial were registered and approved by the Institutional Review Committee of The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University. Ethics number KY-2023-106-01.

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Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling

Lai¹,

Li²,

Ran³

et al. 2023

Biomedicine & Pharmacotherapy

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Comparative study of IgG binding to megakaryocytes in immune and myelodysplastic thrombocytopenic patients

Cited by 3 publications

References 42 publications

Ultrastructural alterations of megakaryocytes in thrombocytopenia: A review of 43 cases

Ultrastructural alterations of megakaryocytes in thrombocytopenia: A review of 43 cases

Gut microbiota alterations in children and their relationship with primary immune thrombocytopenia

Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling

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