Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applications

Wang, Zongjie; Tian, Zhenlin; Menard, Fredric; Kim, Keekyoung

doi:10.1088/1758-5090/aa83cf

Cited by 100 publications

(111 citation statements)

References 53 publications

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“…Furthermore, 3D printing processes have found numerous applications, including biomedical. Fish gelatine, which is more soluble and remains liquid at lower temperatures compared to mammalian gelatines is an good potential candidate for use a a biological dye for use in 3D printing of tissue scaffolds [84]. Visser et al [85] have created reinforced gelatine metha-acrylamide (GelMA) hydrogels with poly(e-caprolactone) (PCL) fiber scaffolds using melt electrospinning direct writing as a form of 3D printing.…”

Section: Opportunities For Novel Applications Of Fish Gelatine and Comentioning

confidence: 99%

Marine Gelatine from Rest Raw Materials

Milovanović¹,

Hayes²

2018

Preprint

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In recent years, demand for consumption of marine foods, and especially fish, has substantially increased worldwide. The majority of collagen available is sourced from mammalian-derived products. Although fish derived gelatine is a viable alternative to mammalian sourced gelatine, there are some challenges related to the use of fish gelatine including odour, colour, gelling and film forming properties as well as consistency in gelatine amino acid composition. Chemicals used for pre-treatment, as well as extraction conditions such as temperature and time, can influence the length of polypeptide chains that result and the functional properties of the gelatine. Compared to mammalian sources, gelatines derived from fish show notable differences in physical and chemical properties, and great care should be paid to optimization of the production process in order to obtain a product with the best properties for intended applications. The focus of this review is to explore the feasibility of producing gelatine sourced from marine processing by-products using different pre-treatment and extraction strategies with the aim of improving the techno-functional properties of the final product and improving the clean-label status of gelatines. The bioactivities of gelatine hydrolysates are also discussed.

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Section: Opportunities For Novel Applications Of Fish Gelatine and Comentioning

confidence: 99%

Marine Gelatine from Rest Raw Materials

Milovanović¹,

Hayes²

2018

Preprint

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“…The degree of crosslinking is calculated by integrating the double bond proton peaks (vinyl) considering methyl peaks. [ 47,48 ] Same procedure is followed to calculate the percentage of crosslinking for the hybrid hydrogel, which was found to be ≈65%.…”

Section: Resultsmentioning

confidence: 99%

“…To quantify the degree of methacrylation, GelMA and AlgMA were separately dissolved in D 2 O at a concentration of 10 mg mL −1 , and the degree of substitution was estimated based on literature. [ 47,48 ] To assess the crosslinking degree of bioadhesives, the hybrid hydrogels containing GelMA (20% w/v) and 3% w/v AlgMA were photocrosslinked according to the previous section. The samples were then immersed in DMSO for 7 days at 37 °C and sonicated for 1 h every day to partially dissolve the hydrogels prior to conducting the NMR spectroscopy.…”

Section: Methodsmentioning

confidence: 99%

Engineering Tough, Injectable, Naturally Derived, Bioadhesive Composite Hydrogels

Tavafoghi

Sheikhi

Tutar

et al. 2020

Adv Healthcare Materials

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Engineering mechanically robust bioadhesive hydrogels that can withstand large strains may open new opportunities for the sutureless sealing of highly stretchable tissues. While typical chemical modifications of hydrogels, such as increasing the functional group density of crosslinkable moieties and blending them with other polymers or nanomaterials have resulted in improved mechanical stiffness, the modified hydrogels have often exhibited increased brittleness resulting in deteriorated sealing capabilities under large strains. Furthermore, highly elastic hydrogels, such as tropoelastin derivatives are highly expensive. Here, gelatin methacryloyl (GelMA) is hybridized with methacrylate‐modified alginate (AlgMA) to enable ion‐induced reversible crosslinking that can dissipate energy under strain. The hybrid hydrogels provide a photocrosslinkable, injectable, and bioadhesive platform with an excellent toughness that can be tailored using divalent cations, such as calcium. This class of hybrid biopolymers with more than 600% improved toughness compared to GelMA may set the stage for durable, mechanically resilient, and cost‐effective tissue sealants. This strategy to increase the toughness of hydrogels may be extended to other crosslinkable polymers with similarly reactive moieties.

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“…to standard procedures. [14][15][16] Human lung adenocarcinomaderived A549 cells were obtained from ATCC Ò and maintained in Dulbecco's modified Eagle's medium (DMEM; Gibco, Life Technologies, Inc., New York, NY) containing 2% foetal bovine serum (FBS; Gibco, Life Technologies, Inc., New York, NY).…”

Section: Introductionmentioning

confidence: 99%

Type II Photoinitiator and Tuneable Poly(Ethylene Glycol)-Based Materials Library for Visible Light Photolithography

Yang

Mina

Bas

et al. 2020

Tissue Engineering Part A

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Stereolithography (SL) has several advantages over traditional biomanufacturing techniques such as fused deposition modeling, including increased speed, accuracy, and efficiency. While SL has been broadly used in tissue engineering for the fabrication of three-dimensional scaffolds that can mimic the in vivo environment for cell growth and tissue regeneration, lithographic printing is usually performed on single-component materials cured with ultraviolet light, severely limiting the versatility and cytocompatibility of such systems. In this Color images are available online. study, we report a highly tunable, low-cost photoinitiator system that we used to establish a systematic library of crosslinked materials based on low molecular weight poly(ethylene glycol) diacrylate. We assessed the physicochemical properties, photocrosslinking efficiency, cost performance, and biocompatibility to demonstrate the capability of manufacturing a multimaterial complex tissue scaffold. Stereolithography (SL) has advantages over traditional biomanufacturing techniques, including accuracy and efficiency. While SL has been broadly used for fabricating three-dimensional scaffolds that can mimic the in vivo environment for cell growth and tissue regeneration, lithographic printing is usually performed on single-component materials cured with ultraviolet light, severely limiting the versatility and cytocompatibility of such systems. In this study, we report a highly tunable photoinitiator system and establish a systematic library of crosslinked materials based on poly(ethylene glycol) diacrylate. We assessed the physicochemical properties, photocrosslinking efficiency and biocompatibility to demonstrate the capability of manufacturing a multimaterial complex tissue scaffold.

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Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applications

Cited by 100 publications

References 53 publications

Marine Gelatine from Rest Raw Materials

Marine Gelatine from Rest Raw Materials

Engineering Tough, Injectable, Naturally Derived, Bioadhesive Composite Hydrogels

Type II Photoinitiator and Tuneable Poly(Ethylene Glycol)-Based Materials Library for Visible Light Photolithography

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