Comparative Study of Fucoidan from Saccharina japonica and Its Depolymerized Fragment on Adriamycin-Induced Nephrotic Syndrome in Rats

Abstract

Nephrotic syndrome (NS) is a clinical syndrome with a variety of causes, mainly characterized by heavy proteinuria, hypoalbuminemia, and edema. At present, identification of effective and less toxic therapeutic interventions for nephrotic syndrome remains to be an important issue. In this study, we isolated fucoidan from Saccharina japonica and prepared its depolymerized fragment by oxidant degradation. Fucoidan and its depolymerized fragment had similar chemical constituents. Their average molecular weights were 136 and 9.5 kDa respectively. The effect of fucoidan and its depolymerized fragment on adriamycin-induced nephrotic syndrome were investigated in a rat model. The results showed that adriamycin-treated rats had heavy proteinuria and increased blood urea nitrogen (BUN), serum creatinine (SCr), total cholesterol (TC), and total triglyceride (TG) levels. Oral administration of fucoidan or low-molecular-weight fucoidan for 30 days could significantly inhibit proteinuria and decrease the elevated BUN, SCr, TG, and TC level in a dose-dependent manner. At the same dose (100 mg/kg), low-molecular-weight fucoidan had higher renoprotective activity than fucoidan. Their protective effect on nephrotic syndrome was partly related to their antioxidant activity. The results suggested that both fucoidan and its depolymerized fragment had excellent protective effect on adriamycin-induced nephrotic syndrome, and might have potential for the treatment of nephrotic syndrome.

Experimental Study on the Mechanism of Cinnamaldehyde Ameliorate Proteinuria Induced by Adriamycin

“…Proteinuria is the main manifestation of NS, as well as the main pathological change in rats of adriamycin-induced NS [ 19 ]. Studies show that RAS is closely related to hypertension and proteinuria [ 20 ], the occurrence of hypertension will in turn aggravate proteinuria, and lowering blood pressure can reduce proteinuria [ 21 ].…”

Metabolomic Analysis of Stephania tetrandra–Astragalus membranaceus Herbal Pair-Improving Nephrotic Syndrome Identifies Activation of IL-13/STAT6 Signaling Pathway

“…Rats in group K were injected with the same volume of normal saline as control. The successful establishment of the nephrotic syndrome model was confirmed by the detection of a large amount of albuminuria in the urine samples of rats in M group [ 43 ]. The M group was further divided into a M ( n = 6), FJ ( n = 6), HQ ( n = 6), FH ( n = 6), and Y ( n = 6).…”

High-sulfated derivative of polysaccharide from Ulva pertusa improves Adriamycin-induced nephrotic syndrome by suppressing oxidative stress

“…Model group animals were administered a single injection of Adriamycin (ADR, 6.0 mg kg −1 , dissolved in 0.9% NaCl solution) by tail vein. 27 The NOR group rats were injected with an equal volume of 0.9% NaCl solution, also by tail vein. Two weeks later, nephrotic syndrome (NS) was successfully established in all model group rats (urine protein ≥100 mg per 24 h).…”