Comparative study of frost tolerance and antioxidant activity in cereals

Janda, Tibor; Szalai, Gabriella; Rios-Gonzalez, Krisztina; Veisz, O.; Páldi, Emil

doi:10.1016/s0168-9452(02)00414-4

Cited by 164 publications

(105 citation statements)

References 25 publications

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“…Significantly higher activity of this enzyme was detected in anthers collected from freshly cut tillers of 'recalcitrant' DH lines in comparison with 'responsive' ones. However, in 'recalcitrant' genotypes LT pre-treatment significantly decreased CAT activity, as it was reported by Taşgin et al (2006) and Janda et al (2003) in several frost-tolerant and frost-sensitive cereal species. According to these authors, it confirmed high cold sensitivity of CAT.…”

Section: Discussionsupporting

confidence: 73%

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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To verify the hypothesis that cell redox status regulates the process of microspore embryogenesis (ME), reactive oxygen species (ROS) generation and the activity of enzymatic and non-enzymatic antioxidants were analyzed in eight doubled haploid lines of triticale with significant differences in embryogenic potential. The analyses were performed in anthers excised from freshly cut tillers (control) and from low temperature (LT) pre-treated tillers (3 weeks at 4°C) in which ME has been initiated. Significant associations between ME effectiveness and the variables studied were found. In control cultures, high superoxide dismutase (SOD) activity appeared crucial for microspore viability. On the other hand, positive though non-linear correlation between ME effectiveness and H 2 O 2 generation, and negative correlation with catalase (CAT) activity suggest that some threshold level of H 2 O 2 is important for successful ME initiation. LT tillers pretreatment significantly increased H 2 O 2 accumulation, which had a negative effect on ME effectiveness. However, even high level of H 2 O 2 did not endanger cell viability as long as the cells exhibited high activity of ROS-decomposing enzymes (SOD, CAT and POX). The ability to sustain antioxidative enzyme activity under cold stress in the dark was another important requirement for high effectiveness of ME, allowing for the generation of the signal initiating microspore reprogramming and simultaneously protecting the cells from the toxic effects of ROS production. The role of antioxidative enzymes cannot be replaced even by high activity of non-enzymatic antioxidants. In conclusion, genetically controlled but environmentally modified cell tolerance to oxidative stress seems to play an important role in triticale ME.

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Section: Discussionsupporting

confidence: 73%

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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“…One unit of PPO activity was defined as the amount of enzyme that caused an increase in absorbance of 0.001/min (Shi et al 2002). POX activity was measured by monitoring the increase in absorbance at 470 nm in 50 mM phosphate buffer (pH 5.5) containing 1 mM guaiacol and 0.5 mM H 2 O 2 (Janda et al 2003). One unit of POX activity was defined as the amount of enzyme that caused an increase in absorbance of 0.01/minute.…”

Section: Methodsmentioning

confidence: 99%

Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria

Tozlu¹,

Mohammadi²,

Kotan³

et al. 2016

Plant Prot. Sci.

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Tozlu E., Mohammadi P., Senol Kotan M., Nadaroglu H., Kotan R. (2016): Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria. Plant Protect. Sci.,.Sclerotinia sclerotiorum (Lib.) de Bary is the causal agent of white mould, stem, and fruit rot diseases on a wide variety of crop plants including cabbage (Brassica oleracea L.) in field and storage. Control of this pathogen by using commercial disease management methods is extremely difficult. Therefore, this study was performed to develop an alternative and effective control method for the diseases by using biocontrol bacteria -Bacillus subtilis (strains TV-6F, TV-17C, TV-12H, BA-140 and EK-7), Bacillus megaterium (strains TV-103B), and Bacillus pumilus (strains RK-103) on Petri plate assays and on red cabbage in pot assays. On Petri plates, all of the tested bacterial strains showed the zone of inhibition against the pathogen fungus ranging 15.00-26.50 mm. Their percentage inhibition rates and lesion length ranged 42.64-79.41% and 0.02-4.50 cm in pot assays, respectively. Consequently, our results indicated that especially B. subtilis strains TV-17C, TV-12H, and TV-6F can be used as bio control agent of S. sclerotiorum in red cabbage production.

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“…The peroxidase activity was determined by colorimetric assay (Janda et al, 2003) through the measurement of changes in absorbance at 470 nm. In a tube, 8 mM guaiacol, 16 mM hydrogen peroxide, 100 mM phosphate buffer pH 7.0 and 20 µL of xylopodium exsudate were combined in a final reaction volume of 2 mL.…”

Section: Peroxidase Activity Determination In Solution and In Gelmentioning

confidence: 99%

Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.)

Pinto¹,

Ribeiro²,

Araujo³

et al. 2015

Afr. J. Biotechnol.

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Umbu plants are drought resistant trees which are able to store water and several other substances into its adapted root, named xylopodium. The exsudate from xilopodium is a natural solution rich in salts, sugars and a little concentration of proteins. In this work, we report the purification of a peroxidase (POX) from umbu xilopodium exsudate by direct extraction from polyacrylamide electrophoresis gels. Umbu POX showed optimum activity at pHs around 6.0 to 7.0 and high thermal resistance after incubation at 70°C for 6 min. POX activity present in crude extracts was more heat-resistant than in its purified form. When assayed with metal ions, umbu POX activity was shown to be inhibited by Mn 2+ and stimulated by Ca 2+ and Mg 2+ ; it was also inhibited by sodium azide (concentrations higher then 1 mM) and not inhibited by either EDTA or tropolone. POX Km values for guaiacol and methylcatechol substrates were 6.83 and 22.25, respectively. The enzyme is proposed to be a guaiacol peroxidase and was seen to be located at higher concentrations in the outermost layers of xylopodium tissue, such as the endoderm.

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Comparative study of frost tolerance and antioxidant activity in cereals

Cited by 164 publications

References 25 publications

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Biological control of Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mould disease in red cabbage, by some bacteria

Purification and characterization of a peroxidase present in xilopodium exsudates of umbu plants (Spondias tuberosa A.)

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