Comparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats models

Slesarev, G. P.; Глухарева, Т. В.; Duru, Kingsley C.; Шевырин, Вадим А.; Lyubyakina, P. N.; Kovaleva, Elena G.

doi:10.15159/ar.21.056

Cited by 2 publications

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“…Among the many types of raw materials, kudzu root and soy molasses wastes/by-products are considered vital sources of isoflavones and have been characterized by the presence of three main isoflavones like puerarin, daidzein, and genistein (Aboushanab et al, 2021). Furthermore, available reports have shown the efficacy of using a single natural solvent for extracting isoflavones compared to other organic or toxic solvents (methanol) (Slesarev et al, 2021). Besides, no reports are available on using green extraction and subsequent organic solvent partitioning to isolate the bioactive compounds in the above-mentioned botanical wastes.…”

Section: Resultsmentioning

confidence: 99%

An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

Duru

Slesarev

Aboushanab

et al. 2022

Industrial Crops and Products

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Section: Resultsmentioning

confidence: 99%

An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

Duru

Slesarev

Aboushanab

et al. 2022

Industrial Crops and Products

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“…The NADES-based ultrasound-assisted extraction procedure was used for the extraction of isoflavones from KR and SM, as described by Dai et al with minor modifications [ 34 , 35 ]. Approximately 1 g of KR and SM was accurately weighed into a 50 mL beaker, to which 20 mL and 30 mL of NADES solution were immediately added, respectively.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab

Шевырин

Slesarev

et al. 2022

Plants

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Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC50) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries.

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Comparative study of extraction of soy molasses isoflavones and in vivo bioconversion of daidzein into S-equol in rats models

Cited by 2 publications

References 0 publications

An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

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