Comparative study of estrogenic potencies of estradiol, tamoxifen, bisphenol-A and resveratrol with two in vitro bioassays

Li, Wen; Seifert, Martin; Xu, Ying; Hock, Bertold

doi:10.1016/s0160-4120(03)00183-1

Cited by 40 publications

(16 citation statements)

References 26 publications

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“…We found that BPA is oestrogenically active and approximately 10,000 times less potent than oestradiol, which is similar to what Li et al (40) have reported. Our testing has also showed that the oestrogenic activity of BPA is concentration dependent since the beta-galactosidase activity with the highest tested BPA concentration of 20 mg L -1 was 1.98 and with the lowest tested concentration of 0.3 mg L -1 it was 1.53 (which corresponds to the activity established in the progesterone control sample).…”

Section: Yes Assaysupporting

confidence: 92%

Efficiency of advanced oxidation processes in lowering bisphenol A toxicity and oestrogenic activity in aqueous samples

Plahuta

Tišler

Toman

et al. 2014

Archives of Industrial Hygiene and Toxicology

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Bisphenol A (BPA) is a well-known endocrine disruptor with adverse oestrogen-like effects eliciting adverse effects in humans and wildlife. For this reason it is necessary to set up an efficient removal of BPA from wastewaters, before they are discharged into surface waters. The aim of this study was to compare the efficiency of BPA removal from aqueous samples with photolytic, photocatalytic, and UV/H 2 O 2 oxidation. BPA solutions were illuminated with different bulbs (halogen; 17 W UV, 254 nm; and 150 W UV, 365 nm) with or without the TiO 2 P-25 catalyst or H 2 O 2 (to accelerate degradation). Acute toxicity and oestrogenic activity of treated samples were determined using luminescent bacteria (Vibrio fischeri), water fleas (Daphnia magna), zebrafish embryos (Danio rerio), and Yeast Estrogen Screen (YES) assay with genetically modified yeast Saccharomyces cerevisiae. The results confirmed that BPA is toxic and oestrogenically active. Chemical analysis showed a reduction of BPA levels after photolytic treatment and 100 % conversion of BPA by photocatalytic and UV/H 2 O 2 oxidation. The toxicity and oestrogenic activity of BPA were largely reduced in photolytically treated samples. Photocatalytic oxidation, however, either did not reduce BPA toxic and oestrogenic effects or even increased them in comparison with the baseline, untreated BPA solution. Our findings suggest that chemical analysis is not sufficient to determine the efficiency of advanced oxidation processes in removing pollutants from water and needs to be complemented with biological tests.

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Section: Yes Assaysupporting

confidence: 92%

Efficiency of advanced oxidation processes in lowering bisphenol A toxicity and oestrogenic activity in aqueous samples

Plahuta

Tišler

Toman

et al. 2014

Archives of Industrial Hygiene and Toxicology

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“…However, there are also naturally occurring phytoestrogens, such as genistein in soy beans and resveratrol in grapes, mulberries and peanuts [2, 3]. Although phytoestrogens exert relatively weak estrogen-like activity [4], exposure to high levels for long periods of time may result in significant endocrine disruption.…”

Section: Introductionmentioning

confidence: 99%

Phytoestrogen Resveratrol Suppresses Steroidogenesis by Rat Adrenocortical Cells by Inhibiting Cytochrome P450 c21-Hydroxylase

et al. 2005

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Background and Aim: The phytoestrogen resveratrol is found in grapes, mulberries and peanuts, all of which are consumed regularly by humans. Resveratrol is also used in chemotherapy against cancer and aging and as a cardioprotectant. The aim of the present study was to characterize the effects of resveratrol on rat adrenal steroidogenesis and to study the underlying mechanism. Methods: Adrenocortical cells were isolated from the adrenal glands of normal male rats (in vitro) and from male rats administered resveratrol in their diet for 12 weeks (ex vivo). Cells from resveratrol-treated and non-treated rats were tested ex vivo for responsiveness to ACTH and cells from normal rats were tested in vitro for responsiveness to ACTH in the presence and absence of resveratrol. Corticosterone and progesterone production were measured by RIA and expression of steroidogenic enzymes analyzed by PAGE/Western blotting. Results: Corticosterone production was inhibited 47% by 50 µM resveratrol in vitro and 20% ex vivo, while progesterone production was elevated to 400% of the control value in in vitro experiments. Resveratrol treatment decreased adrenal cytochrome P450 c21-hydroxylase expression in vivo and cell culture conditions. No changes in cell viability or morphology were caused by exposure to resveratrol in both ex vivo and in vitro experiments. Conclusion: Resveratrol suppresses corticosterone production by primary rat adrenocortical cell cultures in vitro and ex vivo by inhibiting cytochrome P450 c21-hydroxylase.

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“…BPA is an environmental estrogen due to its estrogenic activity [1,2]. Microlevel and even trace-level of BPA will adversely affect animal physiology, reproductive system, and offspring viability [3,4]. Therefore, it is very important and interesting to establish a sensitive, simple, rapid, and accurate method for the determination of BPA.…”

Section: Introductionmentioning

confidence: 99%

Preparation and properties of bisphenol A sensor based on multiwalled carbon nanotubes/Li4Ti5O12-modified electrode

Wang

Yang

et al. 2014

Ionics

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A new electrochemical sensor based on the multiwalled carbon nanotube (MWCNT)/Li 4 Ti 5 O 12 -modified glassy carbon electrode for determination of bisphenol A (BPA) was developed by modifying the surface of a glassy carbon electrode with the prepared composite Li 4 Ti 5 O 12 / MWCNTs. The morphology and structure of the composite were characterized by infrared spectroscopy and scanning electron microscope. The obtained electrochemical sensor Li 4 Ti 5 O 12 /MWCNTs/GCE takes advantages of large specific surface area of the Li 4 Ti 5 O 12 nanostructure, excellent adsorption properties, and electrochemical properties of MWCNTs. Under optimal conditions, the response of the proposed sensor to BPA was linear in the BPA concentration range from 1.0× 10 −7 to 1.0×10 −5 mol L −1 and the detection limit was of 7.8× 10 −8 mol L −1 according to the 3σ rule. The results revealed that the newly developed sensor exhibited high sensitivity and selectivity, excellent electrochemical performance, and fast response to BPA, allowing it to be used for the detection of BPA in actual samples.

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Comparative study of estrogenic potencies of estradiol, tamoxifen, bisphenol-A and resveratrol with two in vitro bioassays

Cited by 40 publications

References 26 publications

Efficiency of advanced oxidation processes in lowering bisphenol A toxicity and oestrogenic activity in aqueous samples

Efficiency of advanced oxidation processes in lowering bisphenol A toxicity and oestrogenic activity in aqueous samples

Phytoestrogen Resveratrol Suppresses Steroidogenesis by Rat Adrenocortical Cells by Inhibiting Cytochrome P450 c21-Hydroxylase

Preparation and properties of bisphenol A sensor based on multiwalled carbon nanotubes/Li4Ti5O12-modified electrode

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