Comparative study of different fluorescent dyes for the detection of proteins on membranes using the peroxyoxalate chemiluminescent reaction

Flow and evaporation cells for the detection of proteins on membranes with the peroxyoxalate chemiluminescent reaction in organic media

“…2C). This sensitivity is significantly higher than the maximum sensitivity obtained with the reaction chamber previously developed in our laboratory [24]. However, considering the continuous replacement of the chemiluminescent reagents that occurs in the reaction chamber containing the blot, we expected a much higher sensitivity.…”

“…As indicated in Section 1, it has been previously shown in our laboratory [23,24] that MDPF-labeled proteins can be chemiexcited by the high energy intermediates produced in the TCPO-H 2 O 2 reaction in acetone. Here, we have investigated whether it is possible to increase the sensitivity of this chemiluminescent method by a continuous renewing of the chemiluminescent reagents in the specially designed cells described in Section 2.3 ( Fig.…”

“…We have previously shown that the energy of the intermediates produced in the TCPO-H 2 O 2 reaction in acetone can be transferred to MDPF and other fluorescent dyes bound to proteins on blots [23,24]. This allowed us to develop a chemiluminescent method for the nonspecific detection of proteins on polyvinylidene difluoride (PVDF) membranes [23].…”

“…This allowed us to develop a chemiluminescent method for the nonspecific detection of proteins on polyvinylidene difluoride (PVDF) membranes [23]. Furthermore, to improve this method, we designed a reaction cell to perform this chemiluminescent reaction with a homogeneous distribution of reagents [24]. The objective of this study is to evaluate the possibility to enhance the sensitivity of this detection system by a continuous renewing of the reagents.…”

A novel [Ag(NH3)2]+ probe for chemiluminescent imaging detection of proteins after polyacrylamide gel electrophoresis

“…To eliminate the risk of missing or misrepresenting levels of proteins that do not blot well, Smejkal et al [13] developed a CL-based method which directly immunodetected proteins in agarose gels by conventional antibodybased procedures, and not on electroblots. In addition, CL detection of total protein profiles has also been reported [14,15], which is based on the CL signals from proteins labeled with the fluorescent reagent 2-methoxy-2,4-diphenyl-3(2H)-fluranone (MDPF), but the procedure of electroblotting to PVDF membranes cannot be eliminated. Because of the high cost of antibodies and the risk of missing proteins during transferring procedures, it is of great importance to develop new methods to detect proteins without transfer and immunoreaction.…”