Comparative Study of Composition, Antioxidant, and Antimicrobial Activities of Essential Oils of Selected Aromatic Plants from Balkan Peninsula

Abstract

The objective of the present study to perform a comparative analysis of the chemical composition, antioxidant, and antimicrobial activities of the essential oils of plant species Hyssopus officinalis, Achillea grandifolia, Achillea crithmifolia, Tanacetum parthenium, Laserpitium latifolium, and Artemisia absinthium from Balkan Peninsula. The chemical analysis of essential oils was performed by using gas chromatography and gas chromatography-mass spectrometry. Monoterpenes were dominant among the recorded components, with camphor in T. parthenium, A. grandifolia, and A. crithmifolia (51.4, 45.4, and 25.4 %, respectively), 1,8-cineole in H. officinalis, A. grandifolia, and A. crithmifolia (49.1, 16.4, and 14.8 %, respectively), and sabinene in L. latifolium and A. absinthium (47.8 and 21.5 %). The antiradical and antioxidant activities were determined by using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging methods. The essential oil of A. grandifolia has shown the highest antioxidant activity [IC50 of 33.575 ± 0.069 mg/mL for 2,2-diphenyl-1-picrylhydrazyl and 2.510 ± 0.036 mg vitamin C/g for the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assay]. The antimicrobial activity against 16 multiresistant pathogenic bacteria isolated from human source material was tested by the broth microdilution assay. The resulting minimum inhibitory concentration/minimum bactericidal concentration values ranged from 4.72 to 93.2 mg/mL. Therefore, the essential oils of the plant species included in this study may be considered to be prospective natural sources of antimicrobial substances, and may contribute as effective agents in the battle against bacterial multiresistance.

Chemical Composition, Antibacterial, Antibiofilm and Synergistic Properties of Essential Oils from Eucalyptus globulus Labill. and Seven Mediterranean Aromatic Plants

“…Medicinal plants are an important source of research for the pharmaceutical and food industries . Particularly, in the past few decades, medicinal and aromatic plants have been studied as prolific providers of natural extracts and compounds with antimicrobial activity . Concretely, essential oils (EOs) have been explored in order to use them as safer substitutes of synthetic food additives or in combination due to their synergistic effects with other antimicrobial agents …”

“…aromatic plants have been studied as prolific providers of natural extracts and compounds with antimicrobial activity. [3] [4] Concretely, essential oils (EOs) have been explored in order to use them as safer substitutes of synthetic food additives or in combination due to their synergistic effects with other antimicrobial agents. [5 -7] It is well-known that the antimicrobial activity of EOs is related to some particular compounds, mainly terpenoids and phenylpropenes with the most active being phenols like carvacrol, thymol and eugenol.…”

A Comparative Study on Antioxidation and Antibacterial Activities Triphala Herb Extracts from Chae Son, Lampang, Thailand

“…Total phenolic insides in the extracts were strong-minded by the modified Folin-Ciocalteu method [13]. The 0.1 mL of the extracts was assorted with 1 ml of Folin-Ciocalteu's reagent into 20 ml calibrated flask.…”

“…The total flavonoid contents of crude extract were determined by the aluminium chloride colourimetric method. 13 In brief, 0.5 mL of crude extracts (100 mg/mL) were made up to 1 mL with MeOH/H 2 O/CH 3 COOH (14:5:1) and then mixed with 4 mL of AlCl 3 reagent solution (4 ml, 133 mg of AlCl 3 and 400 mg of CH 3 COONa dissolved in 100 ml H 2 O). After that, the mixture incubated for 5 min, and the absorbance was measured at 430 nm with UV-VIS spectrophotometer (Shimadzu 1601).…”

Phytochemical Characterization of Achillea grandifolia Friv. Essential Oil and Its Potent Against Oxidative Damage, Acetylcholinesterase and a-Amylase

“…Earlier, infusion of A. grandifolia was reported for antioxidant, total phenol and total flavonoid contents [29]. The methanol extract from A. grandifolia collected from Balkan peninsula was reported to have antibacterial and antioxidant properties [30]. The oil of A. grandifolia collected from Serbia was characterized with high percentage of oxygenated monoterpenes with 1,8-cineole (28.8 %), camphor (22.8 %) and borneol (4.9 %) as the major compounds [31].…”